Papers - KOBAYASHI Jun
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Nitric oxide bioavailability for red blood cell deformability in the microcirculation: A review of recent progress. Reviewed
Jun Kobayashi, Kazuo Ohtake, Isamu Murata, Kunihiro Sonoda.
Nitric Oxide, 129 25 - 29 2022.10
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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薗田 邦博、河野 有華、大竹 一男、清水 彩子、柴 祥子、飯野 汐里、加園 恵三、小林 順
金城学院大学論集自然科学編 2024 2024.09
Authorship：Last author
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L-NAME誘発性高血圧ラットの内皮機能不全と心臓リモデリングに対する魚油摂取の効果
薗田 邦博、河野 有華、大竹 一男、竹之内 康広、柴 祥子、小林 順、加園 恵三
金城学院大学消費生活科学研究所紀要 2024.03
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Comparison of nitrate content and antioxidant capacity in beetroot juice made in Japan and UK Reviewed
Kinjo Gakuin Daigaku Ronshu：Studies in Natural Sciences 20 ( 1 ) 26 - 33 2023.09
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異なるジューサーを用いて作製した野菜果物ジュースの硝酸塩含有量と官能検査の比較 Reviewed
薗田邦博、河野有華、清水彩子、大竹一男、柴祥子、加園恵三、小林 順
金城学院大学消費生活科学研究所紀要 27 ( 1 ) 11 - 20 2023.03
Authorship：Last author Language：Japanese Publishing type：Research paper (bulletin of university, research institution)
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非アルコール性脂肪肝炎モデル動物の肝炎と肝線維化に対するSodium nitriteとCaptoprilの併用投与による影響 Reviewed
河野有華、 薗田邦博、大竹一男、柴祥子、北森一哉、小林順
金城学院大学消費生活科学研究所紀要 27 ( 1 ) 1 - 10 2023.03
Authorship：Last author Language：Japanese Publishing type：Research paper (bulletin of university, research institution)
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Salvianolic acid B improves the survival rate, acute kidney dysfunction, inflammation and NETosis‑mediated antibacterial action in a crush syndrome rat model Reviewed
Isamu Murata, Tsugumi Sugai,Yumiko Murakawa,Yoshiaki Miyamoto, Jun Kobayashi, Yutaka Inoue, Ikuo Kanamoto
Experimental and Therapeutic Medicine 23 ( 5 ) 2022.05
Language：English Publishing type：Research paper (scientific journal) Publisher：SPAMDIDOS PUBLICATIONS
Crush syndrome (CS) is a potentially lethal condition characterized by muscle cell damage resulting from decompression following compression. Patients with CS can develop cardiac failure, kidney dysfunction, shock, systemic inflammation and sepsis. Salvianolic acid B (SalB) has cardiac and kidney protective effects and anti‑oxidative, anti‑inflammatory, anti‑apoptotic and anti‑bacterial properties. The present study aimed to demonstrate the survival benefit of SalB in the CS rat model, which comprised anesthetized rats with bilateral hindlimb compression by a rubber tourniquet for 5 h. The rats examined were randomly divided into four groups: i) Sham; ii) sham treated with SalB; iii) CS rat model without treatment; and iv) CS rat model treated with SalB. Under continuous monitoring and recording of arterial blood pressures, blood and tissue samples were collected for biochemical analyses at designated timepoints before and after reperfusion. SalB administration improved the survival rate, kidney function (by treating shock and metabolic acidosis) and inflammation (by reducing mitochondrial dysfunction and endothelial damage). Reduced incidence of cardiac failure due to hyperkalemia was associated with reduced muscle injury via the prevention of mitochondrial dysfunction. Additionally, indirect antibacterial action by the neutrophil extracellular trap system (NETs) was observed. SalB administration to the CS rat model led to a substantial improvement in survival following CS by decreasing kidney and cardiac dysfunctions, inflammation, and endothelial dysfunction by improving the mitochondrial function and through antibacterial effects via NETs.
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Beneficial Effects of Dietary Nitrite on a Model of Nonalcoholic Steatohepatitis Induced by High-Fat/High-Cholesterol Diets in SHRSP5/Dmcr Rats: A Preliminary Study Reviewed
Kunihiro Sonoda,Yuka Kono,Kazuya Kitamori,Kazuo Ohtake,Sachiko Shiba,Keizo Kasono and Jun Kobayashi
International Journal of Molecular Sciences 23 ( 6 ) 1 - 16 2022.03
Language：English Publishing type：Research paper (scientific journal) Publisher：MDPI
Nonalcoholic steatohepatitis (NASH) is a chronic liver disease that leads to liver cirrhosis and hepatocellular carcinoma. Endothelial dysfunction caused by hepatic lipotoxicity is an underlying NASH pathology observed in the liver and the cardiovascular system. Here, we evaluated the effect of dietary nitrite on a rat NASH model. Stroke-prone, spontaneously hypertensive 5/Dmcr rats were fed a high-fat/high-cholesterol diet to develop the NASH model, with nitrite or captopril (100 mg/L, each) supplementation in drinking water for 8 weeks. The effects of nitrite and captopril were evaluated using immunohistochemical analyses of the liver and heart tissues. Dietary nitrite suppressed liver fibrosis in the rats by reducing oxidative stress, as measured using the protein levels of nicotinamide adenine dinucleotide phosphate oxidase components and inflammatory cell accumulation in the liver. Nitrite lowered the blood pressure in hypertensive NASH rats and suppressed left ventricular chamber enlargement. Similar therapeutic effects were observed in a captopril-treated rat NASH model, suggesting the possibility of a common signaling pathway through which nitrite and captopril improve NASH pathology. In conclusion, dietary nitrite attenuates the development of NASH with cardiovascular involvement in rats and provides an alternative NASH therapeutic strategy.
DOI： 10.3390/ijms23062931
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Lifestyle-mediated nitric oxide boost to prevent SARS-CoV-2 infection: A perspective. Reviewed International journal
Jun Kobayashi
Nitric Oxide 115 55 - 61 2021.08
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal) Publisher：ELSEVIER
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread worldwide and has seriously threatened public health by causing significant morbidity and mortality. Patients with coronavirus disease (COVID-19) with preexisting endothelial dysfunction caused by aging, diabetes, hypertension, and obesity are at high risk for life-threatening thromboembolic complications. This suggests a possibility that reduced endothelial nitric oxide (NO) production and NO bioavailability could be a common underlying pathology for the progression of COVID-19. Increasingly, evidence from experimental and clinical studies of SARS-CoV-2 infection shows that NO inhibits the pathogenesis of COVID-19, including virus entry into host cells, viral replication, host immune response, and subsequent thromboembolic complications. Restoring NO bioavailability may have the potential to be a preventive or early-treatment option for COVID-19. This review aims to provide in-depth discussion of NO bioavailability to prevent SARS-CoV-2 infection, particularly by focusing on lifestyle factors such as nitrate-rich diets, physical exercise, and nasal breathing, which could be easily performed on a daily basis to boost NO bioavailability.
DOI： doi: 10.1016/j.niox.2021.08.001
Other Link： https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8340570/
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Letter to the editor: Effects of inhaled nitric oxide in COVID‐19‐induced ARDS–Is it worthwhile? Reviewed International journal
Jun Kobayashi
Acta Anaesthesiologica Scandinavica 2021.07
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal) Publisher：Wiley
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Effect of dietary nitrite supplementation in fetal growth restriction by NO deficient pregnancy hypertension rats. Reviewed
17 ( 2 ) 3 - 9 2021.03
Language：Japanese Publishing type：Research paper (bulletin of university, research institution)
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Nitrite in breast milk: roles in neonatal pathophysiology. Reviewed International journal
Jun Kobayashi
Pediatric Research 1 - 7 2020.11
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal) Publisher：Nature Publishing Group
Dietary nitrate has beneficial effects on health maintenance and prevention of lifestyle-related diseases in adulthood by serving as an alternative source of nitric oxide (NO) through the enterosalivary nitrate–nitrite–NO pathway, particularly when endogenous NO generation is lacking due to vascular endothelial dysfunction. However, this pathway is not developed in the early postnatal period due to a lack of oral commensal nitrate-reducing bacteria and less saliva production than in adults. To compensate for the decrease in nitrite during this period, colostrum contains the highest amount of nitrite compared with transitional, mature, and even artificial milk, suggesting that colostrum plays an important role in tentatively replenishing nitrite, in addition to involving a nutritional aspect, until the enterosalivary nitrate–nitrite–NO pathway is established. Increasing evidence demonstrates that breast milk rich in nitrite can be effective in the prevention of neonatal infections and gastrointestinal diseases such as infantile hypertrophic pyloric stenosis and necrotizing enterocolitis, suggesting that breastfeeding is advantageous for newborns at risk, given the physiological role of nitrite in the early postnatal period.
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Nitric oxide inhalation as an interventional rescue therapy for COVID-19-induced acute respiratory distress syndrome Reviewed International journal
Jun Kobayashi and Isamu Murata
Annals of Intensive Care 61 2020.05
Language：English Publishing type：Research paper (scientific journal) Publisher：Springer
COVID-19 is an emerging disease of public health concern. While there is no specific recommended treatment for COVID-19, nitric oxide has the potential to be of therapeutic value for managing acute respiratory distress syndrome in patients with COVID-19. However, inhaled nitric oxide has not yet been formally evaluated. Given the extent of the COVID-19 pandemic, and the large numbers of hospitalized patients requiring respiratory support, clinical use of inhaled nitric oxide may become an alternate rescue therapy before extracorporeal membrane oxygenation for the management of acute respiratory distress syndrome in patients with COVID-19.
DOI： https://annalsofintensivecare.springeropen.com/articles/10.1186/s13613-020-00681-9
Other Link： https://annalsofintensivecare.springeropen.com/articles/10.1186/s13613-020-00681-9
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Chewing well during meals may benefit health via the enterosalivary nitrate-nitrite-nitric oxide pathway. Invited Reviewed
Jun Kobayashi
Journal of Gastroenterology and Hepatology Research 8 ( 3 ) 1 - 4 2019.06
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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Icing treatment in rats with crush syndrome can improve survival through reduction of potassium concentration and mitochondrial function disorder effect. Reviewed International journal
Murata I, Inoue S, Kobayashi J, Inoue Y, Kanamoto I.
Experimental and Therapeutic Medicine 2019
Language：English Publishing type：Research paper (scientific journal)
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Molecular regulation of skeletal muscle mass and the contribution of nitric oxide: a review. Reviewed International journal
Kobayashi J, Uchida H, Kofuji A, Ito J, Shimizu M, Kim H, Sekiguchi Y, Kushibe S
FASEB BioAdvances 2019
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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Dietary nitrite attenuates elastase-induced pulmonary emphysema in a mouse model. Reviewed International journal
Sonoda K, Ohtake K, Tagiri M, Hirata M, Tamada H, Uchida H, Ito Junta, Kobayashi J.
Biological and Pharmaceutical Bulletin 41 ( 12 ) 1818 - 1823 2018.12
Language：English Publishing type：Research paper (scientific journal)
Pulmonary emphysema (PE) is a major pathological feature of chronic obstructive pulmonary disease (COPD) and is characterized by proteolytic destruction of the alveolar structure and subsequent inflammation of the respiratory tract. We hypothesized that nitrite attenuates the development of PE via anti-inflammatory actions. PE was induced by intratracheal instillation of porcine pancreas elastase (PPE) in mice. Dietary nitrite dose-dependently (50 and 150 mg/L in drinking water) attenuated emphysematous development and macrophage accumulation in the alveolar parenchyma 21 d after PPE treatment. The present study shows that dietary nitrite might be a possible nutritional strategy in preventing the development of PE in mice.
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Effects of diet and gut environment on the gastrointestinal formation of N-nitroso compounds: a review. Reviewed
Kobayashi J
Nitric Oxide 73 66 - 73 2018.02
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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Nitrite as a pharmacological intervention for the successful treatment of crush syndrome Invited Reviewed
Jun Kobayashi and Isamu Murata
Physiological Reports 6 ( 5 ) 2018
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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Interaction between Nitrite and Nifedipine in blood pressure of L-NAME-induced hypertensive rat. Reviewed
2018
Language：Japanese Publishing type：Research paper (other academic)