Papers - UEMURA Takeshi
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MINDY1 Is a Downstream Target of the Polyamines and Promotes Embryonic Stem Cell Self-Renewal. Reviewed International journal
Christina James, Tian Yun Zhao, Anisa Rahim, Parul Saxena, Nazreen Abdul Muthalif, Takeshi Uemura, Norihiro Tsuneyoshi, Sheena Ong, Kazuei Igarashi, Chin Yan Lim, Norris Ray Dunn, Leah A Vardy
Stem cells (Dayton, Ohio) 36 ( 8 ) 1170 - 1178 2018.08
Language：English Publishing type：Research paper (scientific journal)
Embryonic stem cells have the ability to self-renew or differentiate and these processes are under tight control. We previously reported that the polyamine regulator AMD1 is critical for embryonic stem cell self-renewal. The polyamines putrescine, spermidine, and spermine are essential organic cations that play a role in a wide array of cellular processes. Here, we explore the essential role of the polyamines in the promotion of self-renewal and identify a new stem cell regulator that acts downstream of the polyamines: MINDY1. MINDY1 protein levels are high in embryonic stem cells (ESCs) and are dependent on high polyamine levels. Overexpression of MINDY1 can promote ESC self-renewal in the absence of the usually essential cytokine Leukemia Inhibitory Factor (LIF). MINDY1 protein is prenylated and this modification is required for its ability to promote self-renewal. We go on to show that Mindy1 RNA is targeted for repression by mir-710 during Neural Precursor cell differentiation. Taken together, these data demonstrate that high polyamine levels are required for ESC self-renewal and that they function, in part, through promotion of high MINDY1 levels. Stem Cells 2018;36:1170-1178.
DOI： 10.1002/stem.2830
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Protective Effects of Brain Infarction by N-Acetylcysteine Derivatives. Reviewed International journal
Uemura T, Watanabe K, Ko K, Higashi K, Kogure N, Kitajima M, Takayama H, Takao K, Sugita Y, Sakamoto A, Terui Y, Toida T, Kashiwagi K, Igarashi K
Stroke 49 ( 7 ) 1727 - 1733 2018.07
Language：English Publishing type：Research paper (scientific journal)
BACKGROUND AND PURPOSE: We recently found that acrolein (CH2=CH-CHO) is more strongly involved in brain infarction compared with reactive oxygen species. In this study, we looked for acrolein scavengers with less side effects. METHODS: Photochemically induced thrombosis model mice were prepared by injection of Rose Bengal. Effects of N-acetylcysteine (NAC) derivatives on brain infarction were evaluated using the public domain National Institutes of Health image program. RESULTS: NAC, NAC ethyl ester, and NAC benzyl ester (150 mg/kg) were administered intraperitoneally at the time of induction of ischemia, or these NAC derivatives (50 mg/kg) were administered 3× at 24-h intervals before induction of ischemia and 1 more administration at the time of induction of ischemia. The size of brain infarction decreased in the order NAC benzyl ester>NAC ethyl ester>NAC in both experimental conditions. Detoxification of acrolein occurred through conjugation of acrolein with glutathione, which was catalyzed by glutathione S-transferases, rather than direct conjugation between acrolein and NAC derivatives. The level of glutathione S-transferases at the locus of brain infarction was in the order of administration of NAC benzyl ester>NAC ethyl ester>NAC>no NAC derivatives, suggesting that NAC derivatives stabilize glutathione S-transferases. CONCLUSIONS: The results indicate that detoxification of acrolein by NAC derivatives is caused through glutathione conjugation with acrolein catalyzed by glutathione S-transferases, which can be stabilized by NAC derivatives. This is a new concept of acrolein detoxification by NAC derivatives.
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Acrolein toxicity at advanced age: present and future Reviewed
Kazuei Igarashi, Takeshi Uemura, Keiko Kashiwagi
Amino Acids 50 ( 2 ) 217 - 228 2018.02
Language：English Publishing type：Research paper (scientific journal) Publisher：Springer-Verlag Wien
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Acrolein: An effective biomarker for tissue damage produced from polyamines Reviewed
Kazuei Igarashi, Takeshi Uemura, Keiko Kashiwagi
Methods in Molecular Biology 1694 459 - 468 2018
Language：English Publishing type：Research paper (scientific journal) Publisher：Humana Press Inc.
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加齢による脳梗塞悪化メカニズムと新規脳梗塞予防薬の探索 Invited Reviewed
植村武史、渡辺健太、柏木敬子、五十嵐一衛
未病と抗老化 27 39 - 43 2018
Authorship：Lead author Language：Japanese Publishing type：Research paper (scientific journal)
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Spermine oxidase promotes bile canalicular lumen formation through acrolein production Reviewed
Takeshi Uemura, Tomokazu Takasaka, Kazuei Igarashi, Hiroshi Ikegaya
SCIENTIFIC REPORTS 7 ( 1 ) 14841 - 14841 2017.11
Language：English Publishing type：Research paper (scientific journal)
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Determination of 3-hydroxypropylmercapturic acid in urine by three column-switching high-performance liquid chromatography with electrochemical detection using a diamond electrode Reviewed
Kyohei Higashi, Mana Shibasaki, Kyoshiro Kuni, Takeshi Uemura, Masaaki Waragai, Kenichi Uemura, Kazuei Igarashi, Toshihiko Toida
JOURNAL OF CHROMATOGRAPHY A 1517 79 - 85 2017.09
Language：English Publishing type：Research paper (scientific journal)
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Activation of MMP-9 activity by acrolein in saliva from patients with primary Sjogren's syndrome and its mechanism Reviewed
Takeshi Uemura, Takehiro Suzuki, Ryotaro Saiki, Naoshi Dohmae, Satoshi Ito, Hoyu Takahashi, Toshihiko Toida, Keiko Kashiwagi, Kazuei Igarashi
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY 88 84 - 91 2017.07
Language：English Publishing type：Research paper (scientific journal)
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Time dependent transition of the levels of protein-conjugated acrolein (PC-Acro), IL-6 and CRP in plasma during stroke Reviewed
Madoka Yoshida, Naoki Kato, Takeshi Uemura, Mutsumi Mizoi, Mizuho Nakamura, Ryotaro Saiki, Keisuke Hatano, Kunitomo Sato, Shota Kakizaki, Aya Nakamura, Takuya Ishii, Tohru Terao, Yuichi Murayama, Keiko Kashiwagi, Kazuei Igarashi
eNeurologicalSci 7 18 - 24 2017.06
Language：English Publishing type：Research paper (scientific journal) Publisher：Elsevier B.V.
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Correlation between brain damage, associated biomarkers, and medication in psychiatric inpatients: A cross-sectional study Reviewed
Madoka Yoshida, Tetsuto Kanzaki, Mutsumi Mizoi, Mizuho Nakamura, Takeshi Uemura, Seisuke Mimori, Yoriyasu Uju, Keisuke Sekine, Yukihiro Ishii, Taro Yoshimi, Reiko Yasui, Asuka Yasukawa, Mamoru Sato, Seiko Okamoto, Tetsuya Hisaoka, Masafumi Miura, Shun Kusanishi, Kanako Murakami, Chieko Nakano, Yasuhiko Mizuta, Shunichi Mishima, Tatsuro Hayakawa, Kazumi Tsukada, Keiko Kashiwagi, Kazuei Igarashi
CLINICA CHIMICA ACTA 464 50 - 56 2017.01
Language：English Publishing type：Research paper (scientific journal)
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Decrease in acrolein toxicity based on the decline of polyamine oxidases Reviewed
Takeshi Uemura, Mizuho Nakamura, Akihiko Sakamoto, Takehiro Suzuki, Naoshi Dohmae, Yusuke Terui, Hideyuki Tomitori, Robert A. Casero, Keiko Kashiwagi, Kazuei Igarashi
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY 79 151 - 157 2016.10
Language：English Publishing type：Research paper (scientific journal)
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Aggravation of brain infarction through an increase in acrolein production and a decrease in glutathione with aging Reviewed
Takeshi Uemura, Kenta Watanabe, Misaki Ishibashi, Ryotaro Saiki, Kyoshiro Kuni, Kazuhiro Nishimura, Toshihiko Toida, Keiko Kashiwagi, Kazuei Igarashi
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 473 ( 2 ) 630 - 635 2016.04
Language：English Publishing type：Research paper (scientific journal)
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Toxic acrolein production due to Ca2+ influx by the NMDA receptor during stroke Reviewed
Mizuho Nakamura, Takeshi Uemura, Ryotaro Saiki, Akihiko Sakamoto, Hyerim Park, Kazuhiro Nishimura, Yusuke Terui, Toshihiko Toida, Keiko Kashiwagi, Kazuei Igarashi
ATHEROSCLEROSIS 244 131 - 137 2016.01
Language：English Publishing type：Research paper (scientific journal)
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Increase in acrolein-conjugated immunoglobulins in saliva from patients with primary Sjogren's syndrome Reviewed
Tadao Hirose, Ryotaro Saiki, Takeshi Uemura, Takehiro Suzuki, Naoshi Dohmae, Satoshi Ito, Hoyu Takahashi, Itsuko Ishii, Toshihiko Toida, Keiko Kashiwagi, Kazuei Igarashi
CLINICA CHIMICA ACTA 450 184 - 189 2015.10
Language：English Publishing type：Research paper (scientific journal)
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Raman spectroscopy of human skin: Looking for a quantitative algorithm to reliably estimate human age Reviewed
Giuseppe Pezzotti, Marco Boffelli, Daisuke Miyamori, Takeshi Uemura, Yoshinori Marunaka, Wenliang Zhu, Hiroshi Ikegaya
Journal of Biomedical Optics 20 ( 6 ) 065008 - 065008 2015.06
Language：English Publishing type：Research paper (scientific journal) Publisher：SPIE
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Experimental studies of remarkable monoamine releases and neural resistance to the transient ischemia and reperfusion Reviewed
Kanji Yoshimoto, Akira Namera, Yousuke Arima, Takahiro Nagao, Hiroh Saji, Tomokazu Takasaka, Takeshi Uemura, Yoshihisa Watanabe, Shuichi Ueda, Masataka Nagao
Pathophysiology 21 ( 4 ) 309 - 316 2014.11
Language：English Publishing type：Research paper (scientific journal) Publisher：Elsevier
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Identification of Functional Amino Acid Residues Involved in Polyamine and Agmatine Transport by Human Organic Cation Transporter 2 Reviewed
Kyohei Higashi, Masataka Imamura, Satoshi Fudo, Takeshi Uemura, Ryotaro Saiki, Tyuji Hoshino, Toshihiko Toida, Keiko Kashiwagi, Kazuei Igarashi
PLOS ONE 9 ( 7 ) e102234 - e102234 2014
Language：English Publishing type：Research paper (scientific journal)
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Acetaldehyde-induced cytotoxicity involves induction of spermine oxidase at the transcriptional level Reviewed
Takeshi Uemura, Yuka Tanaka, Kyohei Higashi, Daisuke Miyamori, Tomokazu Takasaka, Tatsuo Nagano, Toshihiko Toida, Kanji Yoshimoto, Kazuei Igarashi, Hiroshi Ikegaya
TOXICOLOGY 310 1 - 7 2013.08
Language：English Publishing type：Research paper (scientific journal)
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Polyamine transport systems in mammalian cells and tissues. Reviewed
Uemura T, Gerner EW
Methods in molecular biology (Clifton, N.J.) 720 339 - 348 2011
Language：English Publishing type：Research paper (scientific journal)
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Identification and Functions of Amino Acid Residues in PotB and PotC Involved in Spermidine Uptake Activity Reviewed
Kyohei Higashi, Yoshiharu Sakamaki, Emiko Herai, Risa Demizu, Takeshi Uemura, Sunil D. Saroj, Risa Zenda, Yusuke Terui, Kazuhiro Nishimura, Toshihiko Toida, Keiko Kashiwagi, Kazuei Igarashi
JOURNAL OF BIOLOGICAL CHEMISTRY 285 ( 50 ) 39061 - 39069 2010.12
Language：English Publishing type：Research paper (scientific journal)