植村 武史 (ウエムラ タケシ)

UEMURA Takeshi

写真a

出身大学 【 表示 / 非表示

  • 1996年04月
    -
    2000年03月

    東邦大学   薬学部   薬学科   卒業

出身大学院 【 表示 / 非表示

  • 2002年04月
    -
    2005年03月

    千葉大学  薬学研究科  先進医療科学専攻博士課程  博士課程  修了

  • 2000年04月
    -
    2002年03月

    千葉大学  薬学研究科  医療薬学専攻博士前期課程  修士課程  修了

留学歴 【 表示 / 非表示

  • 2007年10月
    -
    2012年03月

    アリゾナ大学アリゾナがんセンター   博士研究員

取得学位 【 表示 / 非表示

  • 千葉大学 -  博士(医薬学)

  • 千葉大学 -  修士(臨床薬学)

  • 東邦大学 -  学士(薬学)

学外略歴 【 表示 / 非表示

  • 2014年10月
    -
    継続中

      京都府立医科大学   大学院医学研究科   大学等非常勤研究員

  • 2014年10月
    -
    2021年03月

      千葉大学   大学院薬学研究院   大学等非常勤研究員

  • 2014年10月
    -
    2021年03月

      株式会社アミンファーマ研究所   主任研究員

  • 2012年04月
    -
    2014年09月

      京都府立医科大学   大学院医学研究科   助教

  • 2007年10月
    -
    2012年03月

      アリゾナ大学   アリゾナがんセンター   研究員

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所属学会・委員会 【 表示 / 非表示

  • 2019年04月
    -
    継続中
     

    日本分子生物学会

  • 2019年02月
    -
    継続中
     

    American Society for Biochemistry and Molecular Biology

  • 2010年06月
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    継続中
     

    日本ポリアミン学会

  • 2004年04月
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    継続中
     

    日本生化学会

  • 2002年04月
    -
    継続中
     

    日本薬学会

専門分野(科研費分類) 【 表示 / 非表示

  • 生物系薬学

取得資格 【 表示 / 非表示

  • 薬剤師

  • TOEIC(730点~)

 

論文 【 表示 / 非表示

  • The Polyamine Regulator AMD1 Upregulates Spermine Levels to Drive Epidermal Differentiation.

    Rahim Anisa B, Lim Hui Kheng, Tan Christina Yan Ru, Jia Li, Leo Vonny Ivon, Uemura Takeshi, Hardman-Smart Jonathan, Common John E A, Lim Thiam Chye, Bellanger Sophie, Paus Ralf, Igarashi Kazuei, Yang Henry, Vardy Leah A

    J Invest Dermatol   141 ( 9 ) 2178 - 2188.e6   2021年09月

    共著

    Maintaining tissue homeostasis depends on a balance between cell proliferation, differentiation, and apoptosis. Within the epidermis, the levels of the polyamines putrescine, spermidine, and spermine are altered in many different skin conditions, yet their role in epidermal tissue homeostasis is poorly understood. We identify the polyamine regulator, Adenosylmethionine decarboxylase 1 (AMD1), as a crucial regulator of keratinocyte (KC) differentiation. AMD1 protein is upregulated on differentiation and is highly expressed in the suprabasal layers of the human epidermis. During KC differentiation, elevated AMD1 promotes decreased putrescine and increased spermine levels. Knockdown or inhibition of AMD1 results in reduced spermine levels and inhibition of KC differentiation. Supplementing AMD1-knockdown KCs with exogenous spermidine or spermine rescued aberrant differentiation. We show that the polyamine shift is critical for the regulation of key transcription factors and signaling proteins that drive KC differentiation, including KLF4 and ZNF750. These findings show that human KCs use controlled changes in polyamine levels to modulate gene expression to drive cellular behavior changes. Modulation of polyamine levels during epidermal differentiation could impact skin barrier formation or can be used in the treatment of hyperproliferative skin disorders.

    DOI PubMed

  • Polyamine-Rich Diet Elevates Blood Spermine Levels and Inhibits Pro-Inflammatory Status: An Interventional Study.

    Soda Kuniyasu, Uemura Takeshi, Sanayama Hidenori, Igarashi Kazuei, Fukui Taro

    Med Sci (Basel)   9 ( 2 )   2021年03月

    共著

    The Japanese diet and the Mediterranean diet are rich in polyamines (spermidine and spermine). Increased polyamine intake elevated blood spermine levels, inhibited aging-associated pro-inflammatory status (increases in lymphocyte function-associated antigen-1 (LFA-1) on immune cells), suppressed aberrant gene methylation and extended the lifespan of mice. To test the effects of increased polyamine intake by humans, 30 healthy male volunteers were asked to eat polyamine-rich and ready-to-eat traditional Japanese food (natto) for 12 months. Natto with high polyamine content was used. Another 27 male volunteers were asked not to change their dietary pattern as a control group. The volunteers' age of intervention and control groups ranged from 40 to 69 years (median 48.9 ± 7.9). Two subjects in the control group subsequently dropped out of the study. The estimated increases in spermidine and spermine intakes were 96.63 ± 47.70 and 22.00 ± 9.56 µmol per day in the intervention group, while no changes were observed in the control group. The mean blood spermine level in the intervention group gradually rose to 1.12 ± 0.29 times the pre-intervention level after 12 months, and were significantly higher (p = 0.019) than those in the control group. Blood spermidine did not increase in either group. LFA-1 on monocytes decreased gradually in the intervention group, and there was an inverse association between changes in spermine concentrations relative to spermidine and changes in LFA-1 levels. Contingency table analysis revealed that the odds ratio to decrease LFA-1 by increased polyamine intake was 3.927 (95% CI 1.116-13.715) (p = 0.032) when the effect of acute inflammation was excluded. The results in the study were similar to those of our animal experiments. Since methylation changes of the entire genome are associated with aging-associated pathologies and our previous studies showed that spermine-induced LFA-1 suppression was associated with the inhibition of aberrant gene methylation, the results suggest that dietary polyamine contributes to human health and longevity.

    DOI PubMed

  • A Raman algorithm to estimate human age from protein structural variations in autopsy skin samples: a protein biological clock.

    Miyamori Daisuke, Uemura Takeshi, Zhu Wenliang, Fujikawa Kei, Nakaya Takaaki, Teramukai Satoshi, Pezzotti Giuseppe, Ikegaya Hiroshi

    Sci Rep   11 ( 1 ) 5949 - 5949   2021年03月

    共著

    The recent increase of the number of unidentified cadavers has become a serious problem throughout the world. As a simple and objective method for age estimation, we attempted to utilize Raman spectrometry for forensic identification. Raman spectroscopy is an optical-based vibrational spectroscopic technique that provides detailed information regarding a sample's molecular composition and structures. Building upon our previous proof-of-concept study, we measured the Raman spectra of abdominal skin samples from 132 autopsy cases and the protein-folding intensity ratio, R(PF), defined as the ratio between the Raman signals from a random coil an α-helix. There was a strong negative correlation between age and R(PF) with a Pearson correlation coefficient of r = 0.878. Four models, based on linear (R(PF)), squared (R(PF)(2)), sex, and R(PF) by sex interaction terms, were examined. The results of cross validation suggested that the second model including linear and squared terms was the best model with the lowest root mean squared error (11.3 years of age) and the highest coefficient of determination (0.743). Our results indicate that the there was a high correlation between the age and R(PF) and the Raman biological clock of protein folding can be used as a simple and objective forensic age estimation method for unidentified cadavers.

    DOI PubMed

  • Translational Regulation of Clock Genes BMAL1 and REV-ERBα by Polyamines.

    Sakamoto Akihiko, Terui Yusuke, Uemura Takeshi, Igarashi Kazuei, Kashiwagi Keiko

    Int J Mol Sci   22 ( 3 )   2021年01月

    共著

    Polyamines stimulate the synthesis of specific proteins at the level of translation, and the genes encoding these proteins are termed as the "polyamine modulon". The circadian clock generates daily rhythms in mammalian physiology and behavior. We investigated the role of polyamines in the circadian rhythm using control and polyamine-reduced NIH3T3 cells. The intracellular polyamines exhibited a rhythm with a period of about 24 h. In the polyamine-reduced NIH3T3 cells, the circadian period of circadian clock genes was lengthened and the synthesis of BMAL1 and REV-ERBα was significantly reduced at the translation level. Thus, the mechanism of polyamine stimulation of these protein syntheses was analyzed using NIH3T3 cells transiently transfected with genes encoding enhanced green fluorescent protein (EGFP) fusion mRNA with normal or mutated 5'-untranslated region (5'-UTR) of Bmal1 or Rev-erbα mRNA. It was found that polyamines stimulated BMAL1 and REV-ERBα synthesis through the enhancement of ribosomal shunting during the ribosome shunting within the 5'-UTR of mRNAs. Accordingly, the genes encoding Bmal1 and Rev-erbα were identified as the members of "polyamine modulon", and these two proteins are significantly involved in the circadian rhythm control.

    DOI PubMed

  • The Polyamine Putrescine Promotes Human Epidermal Melanogenesis.

    Sridharan Aishwarya, Shi Meng, Leo Vonny Ivon, Subramaniam Nagavidya, Lim Thiam Chye, Uemura Takeshi, Igarashi Kazuei, Tien Guan Steven Thng, Tan Nguan Soon, Vardy Leah A

    J Invest Dermatol   140 ( 10 ) 2032 - 2040.e1   2020年10月

    共著

    Hyperpigmentary conditions can arise when melanogenesis in the epidermis is misregulated. Understanding the pathways underlying melanogenesis is essential for the development of effective treatments. Here, we report that a group of metabolites called polyamines are important in the control of melanogenesis in human skin. Polyamines are cationic molecules present in all cells and are essential for cellular function. We report that polyamine regulator ODC1 is upregulated in melanocytes from melasma lesional skin. We report that the polyamine putrescine can promote pigmentation in human skin explants and primary normal human epidermal melanocytes through induction of tyrosinase which is rate-limiting for the synthesis of melanin. Putrescine supplementation on normal human epidermal melanocytes results in the activation of polyamine catabolism, which results in increased intracellular H(2)O(2.) Polyamine catabolism is also increased in human skin explants that have been treated with putrescine. We further report that inhibition of polyamine catabolism prevents putrescine-induced promotion of tyrosinase levels and pigmentation in normal human epidermal melanocytes, showing that polyamine catabolism is responsible for the putrescine induction of melanogenesis. Our data showing that putrescine promotes pigmentation has important consequences for hyperpigmented and hypopigmented conditions. Further understanding of how polyamines control epidermal pigmentation could open the door for the development of new therapeutics.

    DOI PubMed

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学術関係受賞 【 表示 / 非表示

  • 博慈会 ダ・ビンチ賞

    2018年11月01日   博慈会  

    受賞者:  植村武史

 

担当経験のある授業科目(学外) 【 表示 / 非表示

  • 警察学校   中毒学

  • 千葉大学   分析化学III

  • 京都府立医科大学   医学概論実習I

  • 京都府立医科大学   医学研究方法概論

  • 京都府立医科大学   法医学

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学会・委員会等活動 【 表示 / 非表示

  • 2014年10月
    -
    継続中

    日本ポリアミン学会   企画運営委員会委員、学会誌編集委員