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Affiliation |
Faculty of Pharmaceutical Sciences Department of Pharmaceutical Sciences |
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External Link |
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UEMURA Takeshi
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Degree 【 display / non-display 】
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博士（医薬学） ( 2005.03 千葉大学 )
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学士（薬学） ( 2000.03 東邦大学 )
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修士（臨床薬学） ( 2000.03 千葉大学 )
Research Areas 【 display / non-display 】
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Life Science / Pharmaceutical hygiene and biochemistry
From School 【 display / non-display 】
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Toho University Faculty of Pharmaceutical Science Graduated
1996.04 - 2000.03
Country：Japan
From Graduate School 【 display / non-display 】
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Chiba University Graduate School, Division of Pharmaceutical Sciences Doctor's Course Completed
2002.04 - 2005.03
Country：Japan
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Chiba University Graduate School, Division of Pharmaceutical Sciences Master's Course Completed
2000.04 - 2002.03
Country：Japan
Studying abroad experiences 【 display / non-display 】
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2007.10 - 2012.03 University of Arizona, Arizona Cancer Center Postdoctoral Research Fellow
External Career 【 display / non-display 】
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Kyoto Prefectural University of Medicine Part-time researcher for university or other academic organization
2014.10
Country：Japan
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Chiba University Part-time researcher for university or other academic organization
2014.10 - 2021.03
Country：Japan
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株式会社アミンファーマ研究所 主任研究員
2014.10 - 2021.03
Country：Japan
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Kyoto Prefectural University of Medicine Assistant Professor
2012.04 - 2014.09
Country：Japan
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University of Arizona Arizona Cancer Center Researcher
2007.10 - 2012.03
Country：United States
Professional Memberships 【 display / non-display 】
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日本分子生物学会
2019.04
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American Society for Biochemistry and Molecular Biology
2019.02
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日本ポリアミン学会
2010.06
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日本生化学会
2004.04
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日本薬学会
2002.04
Papers 【 display / non-display 】
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Correlation of polyamines, acrolein-conjugated lysine and polyamine metabolic enzyme levels with age in human liver. Reviewed
Uemura Takeshi, Akasaka Yoshihisa, Ikegaya Hiroshi
Heliyon 6 ( 9 ) e05031 - e05031 2020.09
Authorship：Lead author, Corresponding author Language：English Publishing type：Research paper (scientific journal)
The polyamines spermidine, spermine and putrescine are essential for normal cellular functions. The contents of polyamines in tissue decreased in aged mice compared to young mice. In this study, the polyamine contents and their metabolic byproduct acrolein-conjugated lysine (N (ε)-(3-formyl-3,4-dehydropiperidino)-lysine, FDP-Lys) in human liver tissue were measured and analyzed the correlation with age of the subjects. The putrescine and FDP-Lys levels were significantly increased with age. On the other hand, spermine level was decreased with age. Spermidine did not significantly correlate with age. The relative amount of spermine oxidase (SMOX) significantly correlated with the age of subjects whereas ornithine decarboxylase (ODC) and adenosylmethionine decarboxylase (AMD1) significantly reduced by the age. Our results suggested that an increase in oxidation and reduction in polyamine synthesis may cause the change of polyamine profile in the elderly.
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A search for acrolein scavengers among food components Reviewed
Uemura T, Uchida M, Nakamura M, Shimekake M, Sakamoto A, Terui Y, Higashi K, Ishii I, Kashiwagi K, Igarashi K.
Amino Acids 2023.02
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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Nα-(5-Fluoro-2,4-dinitrophenyl)-L-leucinamide-derivatized LC/MS/MS Analysis of Amino Acid Enantiomers in HepG2 Cells Reviewed
Takano Y, Takahashi M, Kobayashi M, Uemura T, Furuchi T.
Chromatography 42 ( 3 ) 143 - 149 2021
Language：English Publishing type：Research paper (scientific journal)
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Structural change and degradation of cytoskeleton due to the acrolein conjugation with vimentin and actin during brain infarction. Reviewed
Uemura Takeshi, Suzuki Takehiro, Ko Kenta, Nakamura Mizuho, Dohmae Naoshi, Sakamoto Akihiko, Terui Yusuke, Toida Toshihiko, Kashiwagi Keiko, Igarashi Kazuei
Cytoskeleton (Hoboken) 77 ( 10 ) 414 - 421 2020.10
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
We have found recently that dendritic spine extension is inhibited through acrolein conjugation with α- and β-tubulin proteins during brain infarction. In this current study, we looked for other acrolein-conjugated proteins in the 100,000g precipitate fraction, to clarify how cytoskeleton structure is modified by acrolein. Acrolein-conjugated proteins were sought from acrolein-treated mouse FM3A and Neuro2a cells and from tissues isolated from mouse brain infarction. It was found that vimentin was conjugated with acrolein, and the conjugated amino acid residue was Cys328, which is the only Cys residue in vimentin. It was also found that Cys207, 257, 285, and Lys118 in actin, another cytoskeleton protein, were conjugated with acrolein. The structure and localization of vimentin and actin filaments were changed greatly in infarct brain in photochemically induced thrombosis model mice and in acrolein-treated Neuro2a cells. In addition, degradation of cytoskeleton proteins was accelerated in the order vimentin > tubulin > actin in mouse brain infarction. These findings indicate that a dysfunction of the cytoskeleton by acrolein is strongly involved in the tissue damage during brain infarction, together with the apoptosis caused by glyceraldehyde-3-phosphate dehydrogenase and protein degradation by matrix metalloproteinase-9.
DOI： 10.1002/cm.21638
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Urinary Amino Acid-Conjugated Acrolein and Taurine as New Biomarkers for Detection of Dementia Reviewed
Yoshida M, Uemura T, Mizoi M, Waragai M, Sakamoto A, Terui Y, Kashiwagi K, Igarashi K.
J Alzheimers Dis. 2023.01
Authorship：Second author Language：English Publishing type：Research paper (scientific journal)
Presentations 【 display / non-display 】
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Aging associated change in polyamine metabolism Invited International conference
Takeshi Uemura, Yuka Yokota, Miki Matsunaga, Yoshihisa Akasaka, Koichi Takao, Hiroshi Ikegaya, Takemitsu Furuchi
Sixth International Conference on Polyamines: Biochemical, Physiological and Clinical Perspectives 2022.09 Enzo Agostinelli
Event date： 2022.09
Language：English Presentation type：Oral presentation (general)
Venue：Tivoli, Rome Country：Italy
Awards 【 display / non-display 】
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博慈会 ダ・ビンチ賞
2018.11 博慈会
植村武史
Award type：Honored in official journal of a scientific society, scientific journal Country：Japan
Scientific Research Funds Acquisition Results 【 display / non-display 】
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タンパク質翻訳合成およびアミン類代謝経路の補正による抗老化機構の解明
Grant number：22H03515 2022.04 - 2024.03
基盤研究(B)
Authorship：Coinvestigator(s) Grant type：Competitive
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ポリアミンが誘導するリピート病の発症機構とその制御による病態抑制効果の解明
Grant number：22K07357 2022.04 - 2024.03
基盤研究(C)
Authorship：Coinvestigator(s) Grant type：Competitive
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加齢によるスペルミン分解活性化機構解明と新規抗老化物質の創出
Grant number：22K11860 2022.04 - 2024.03
基盤研究(C)
Authorship：Principal investigator Grant type：Competitive
Teaching Experience 【 display / non-display 】
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中毒学
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医学研究方法概論
Institution：Kyoto Prefectural University of Medicine
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分析化学III
Institution：Chiba University
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法医学
Institution：Kyoto Prefectural University of Medicine
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法医学実習
Institution：Kyoto Prefectural University of Medicine
Committee Memberships 【 display / non-display 】
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日本ポリアミン学会 企画運営委員会委員、学会誌編集委員
2014.10
Committee type：Academic society