Papers - KIMIRA Yoshifumi
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Uekawa A, Yamanaka H, Lieben L, Kimira Y, Uehara M, Yamamoto Y, Kato S, Ito K, Carmeliet G, Masuyama R
The FASEB Journal 2017.12
Language：English Publishing type：Research paper (scientific journal)
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Collagen-derived dipeptide prolyl-hydroxyproline promotes osteogenic differentiation through Foxg1 Reviewed
Yoshifumi Kimira, Haruka Odaira, Kaho Nomura, Yuri Taniuchi, Naoki Inoue, Sachie Nakatan, Jun Shimizu, Masahiro Wada, Hiroshi Mano
Cellular & Molecular Biology Letters 22 ( 27 ) 2017.12
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
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Mangiferin positively regulates osteoblast differentiation and suppresses osteoclast differentiation Reviewed
Yuusuke Sekiguchi Hiroshi Mano Sachie Nakatani Jun Shimizu Aya Kataoka Kana Ogura Yoshifumi Kimira Midori Ebata Masahiro Wada
Molecular Medicine Reports 2017.06
Language：English Publishing type：Research paper (scientific journal)
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Association between skin condition and sleep efficiency in Japanese young adults Reviewed
Yoshizaki T, Kimira Y, Mano H, Ota M, Iwatsuki K, Oishi Y, Yamane T
Journal of Nutritional Science and Vitaminology 2016.09
Authorship：Second author Language：English Publishing type：Research paper (scientific journal)
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Shiga Takahiro, Kimira Yoshifumi, Mano Hiroshi, Kawata Tetsunori, Tadokoro Tadahiro, Suzuki Tsukasa, Yamamoto Yuji
Biosci Biotechnol Biochem 80 ( 3 ) 510 - 513 2016.03
Language：English Publishing type：Research paper (scientific journal)
Vitamin B12 deficiency is a risk factor for bone disorders via mechanisms not fully understood. In this study, an increase in serum inorganic phosphorus (Pi) concentrations was associated with a vitamin B12 deficiency. Napi2a, a renal cotransporter for Pi reabsorption, accumulated on plasma membranes in a vitamin B12 deficiency suggests that vitamin B12 plays an important role in Pi homeostasis.
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Kimira Yoshifumi, Taniuchi Yuri, Nakatani Sachie, Sekiguchi Yuusuke, Kim Hyoun Ju, Shimizu Jun, Ebata Midori, Wada Masahiro, Matsumoto Akiyo, Mano Hiroshi
Phytomedicine 22 ( 12 ) 1120 - 1124 2015.11
Language：English Publishing type：Research paper (scientific journal)
BACKGROUND: Animal experiment studies have revealed a positive association between intake of citrus fruits and bone health. Nomilin, a limonoid present in citrus fruits, is reported to have many biological activities in mammalian systems, but the mechanism of nomilin on bone metabolism regulation is currently unclear. PURPOSE: To reveal the mechanism of nomilin on osteoclastic differentiation of mouse primary bone marrow-derived macrophages (BMMs) and the mouse RAW 264.7 macrophage cell line into osteoclasts. STUDY DESIGN: Controlled laboratory study. Effects of nomilin on osteoclastic differentiation were studied in in vitro cell cultures. METHODS: Cell viability of RAW 264.7 cells and BMMs was measured with the Cell Counting Kit. TRAP-positive multinucleated cells were counted as osteoclast cell numbers. The number and area of resorption pits were measured as bone-resorbing activity. Osteoclast-specific genes expression was evaluated by quantitative real-time PCR; and proteins expression was evaluated by western blot. RESULTS: Nomilin significantly decreased TRAP-positive multinucleated cell numbers compared with the control, and exhibited no cytotoxicity. Nomilin decreased bone resorption activity. Nomilin downregulated osteoclast-specific genes, NFATc1 and TRAP mRNA levels. Furthermore, nomilin suppressed MAPK signaling pathways. CONCLUSION: This study demonstrates clearly that nomilin has inhibitory effects on osteoclastic differentiation in vitro. These findings indicate that nomilin-containing herbal preparations have potential utility for the prevention of bone metabolic diseases.
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Age-related decrease of IF5/BTG4 in oral and respiratory cavities in mice. Reviewed
Mano Hiroshi, Nakatani Sachie, Kimira Yoshifumi, Mano Mikiko, Sekiguchi Yuusuke, Im Ryang-Hyock, Shimizu Jun, Wada Masahiro
Biosci Biotechnol Biochem 79 ( 6 ) 960 - 968 2015.06
Language：English Publishing type：Research paper (scientific journal)
An IF5 cDNA was isolated by expression cloning from a mouse oocyte cDNA library. It encoded a protein of 250 amino acids, and the region of it encoding amino acids 1-137 showed 86.8% alignment with the anti-proliferative domain of BTG/TOB family genes. This gene is also termed BTG4 or PC3B. Transiently expressed IF5/BTG4 induced alkaline phosphatase activity in human embryonic kidney (HEK293T) and 2T3 cells. IF5/BTG4 mRNA was detected by reverse transcription polymerase chain reaction in pharynx, larynx, trachea, oviduct, ovary, caput epididymis, and testis, but not in lung, intestine, or liver. Immunohistochemistry showed the IF5/BTG4 protein to be present in epithelial cells of the tongue, palate, pharynx, internal nose, and trachea. Both protein and mRNA levels of IF5/BTG4 were reduced by aging when comparing 4-week-old mice with 48-week-old mice. Our findings suggest that IF5/BTG4 may be an aging-related gene in epithelial cells.
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Shimizu Jun, Asami Naoto, Kataoka Aya, Sugihara Fumihito, Inoue Naoki, Kimira Yoshifumi, Wada Masahiro, Mano Hiroshi
Biochem Biophys Res Commun 456 ( 2 ) 626 - 630 2015.01
Language：English Publishing type：Research paper (scientific journal)
Oral supplementation with collagen hydrolysate (CH) has been shown to improve the condition of the skin in humans and experimental animals. Several hydroxyproline-containing oligo-peptides were previously detected in human peripheral blood after the ingestion of CH, and the two dipeptides, prolyl-hydroxyproline (PO) and hydroxyprolyl-glycine (OG), have been proposed to have beneficial effects on human health. When HR-1 hairless mice were fed a HR-AD diet, which lacked magnesium and zinc, transepidermal water loss (TEWL) increased and water content of stratum corneum decreased. In the present study, we investigated the effects of dietary PO and OG on skin barrier dysfunction in HR-1 hairless mice. Mice were fed a HR-AD diet with or without PO (0.15%) and OG (0.15%) for 35 consecutive days. The administration of PO and OG significantly decreased TEWL, and significantly increased water content of stratum corneum. A DNA microarray analysis of the dorsal skin revealed differences in gene expression between the group administered PO and OG and the control group. We also identified muscle-related Gene Ontology as a result of analyzing the up-regulated genes. These results suggested that the administration of PO and OG improved skin barrier dysfunction and altered muscle-related gene expression.
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Kimira Yoshifumi, Ogura Kana, Taniuchi Yuri, Kataoka Aya, Inoue Naoki, Sugihara Fumihito, Nakatani Sachie, Shimizu Jun, Wada Masahiro, Mano Hiroshi
Biochem Biophys Res Commun 453 ( 3 ) 498 - 501 2014.10
Language：English Publishing type：Research paper (scientific journal)
Prolyl-hydroxyproline (Pro-Hyp) is one of the major constituents of collagen-derived dipeptides. The objective of this study was to investigate the effects of Pro-Hyp on the proliferation and differentiation of MC3T3-E1 osteoblastic cells. Addition of Pro-Hyp did not affect MC3T3-E1 cell proliferation and matrix mineralization but alkaline phosphatase activity was significantly increased. Furthermore, cells treated with Pro-Hyp significantly upregulated gene expression of Runx2, Osterix, and Col1alpha1. These results indicate that Pro-Hyp promotes osteoblast differentiation. This study demonstrates for the first time that Pro-Hyp has a positive effect on osteoblast differentiation with upregulation of Runx2, Osterix, and Collalpha1 gene expression.
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Transcriptome of ATDC5 Cultured with Glucosamine Hydrochloride and Collagen Hydrolysate Indicates a New Candidate Gene for the Differentiation of Chondrocytes Reviewed
2 ( 3 ) 233 - 237 2014.09
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コラーゲンペプチドの食品機能性 Reviewed
君羅好史, 真野博
日本食生活学会誌 2014
Authorship：Lead author Language：Japanese Publishing type：Research paper (scientific journal)
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人工容器での培養開始時期がニホンウズラ胚の外部計測値とカルシウム及びマグネシウムの含有量に与える影響 Reviewed
福永一朗, 佐々木剛, 安藤元一, 君羅好史, 上原万里子, 橋本光一郎, 小川博
東京農業大学農学集報 2013.06
Language：Japanese Publishing type：Research paper (bulletin of university, research institution)
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Tousen Yuko, Uehara Mariko, Abe Fumiko, Kimira Yoshifumi, Ishimi Yoshiko
Nutr J 12 127 - 127 2013
Language：English Publishing type：Research paper (scientific journal)
BACKGROUND: Recent studies suggest that some of the clinical effectiveness of soy or daidzein, which is a type of isoflavone, may be attributed to a person's ability to produce equol from daidzein. Equol, which is a metabolite of one of the major soybean isoflavones called daidzein, is produced in the gastrointestinal tract by certain intestinal microbiota where present. Habitual dietary patterns may alter the intestinal bacterial profile, and influence the metabolism of isoflavones and the production of equol. Fructooligosaccharides (FOS) have a prebiotic activity as well as being a dietary fibre. The purpose of the present study was to determine whether FOS supplementation increases equol production in equol producers and stimulates equol production in equol non-producers in Japanese postmenopausal women. METHODS: A soy challenge was used to assess equol-producer status prior to the start of the study in healthy postmenopausal Japanese women. The study involved 4 separate groups in randomised crossover design. First, subjects were classified as equol producers (n = 25) or non-producers (n = 18), and then they were randomly assigned to the FOS or control group. All subjects received a daily dose of 37 mg isoflavone conjugates in the capsule (21 mg aglycone form) and either FOS (5 g/day) or sucrose as control, in a randomised crossover study design. Equol -production was assessed by testing the serum and urine before and after the 2-week supplementation period. RESULTS: The analyses were conducted on 34 subjects completed the study, 21 (61.8%) were classified as equol producers, and 13 (38.2%) as non-producers. Significant differences were observed in the interaction effect of time x equol state after 1 week of intervention (p = 0.006). However there were no effects after 2 weeks of intervention (p = 0.516). Finally, in both equol producers and non-producers, FOS supplementation did not affect the serum equol concentration or the urinary equol to daidzein concentration ratios. CONCLUSIONS: We have reported that FOS intervention (5 g/day for 2 weeks) does not significantly modulate the capacity of intestinal microbiota to produce equol in postmenopausal Japanese women, in either equol producers or non-producers in this pilot study. Further larger investigations that explore the roles of specific intestinal microbiota in equol production will enable the establishment of dietary conditions that are required to enhance equol production.
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Kimira Yoshifumi, Tajima Kiyono, Ohta Atsutane, Ishimi Yoshiko, Katsumata Shin-Ichi, Suzuki Kazuharu, Adlercreutz Herman, Uehara Mariko
J Clin Biochem Nutr 51 ( 2 ) 156 - 160 2012.09
Language：English Publishing type：Research paper (scientific journal)
Fructooligosaccharides stimulate the growth of Bifidobacteria, which cleave isoflavone glycosides to yield corresponding aglycones, and convert metabolites by enhancing enterohepatic recirculation of isoflavones in rats. In the present study, we determined the synergistic effect of dietary isoflavone glycosides and fructooligosaccharides on postgastrectomy osteopenia in rats. Nine-week-old male Sprague-Dawley rats were gastrectomized (n = 20) or sham operated, (control, n = 5) and then randomly assigned to 5 diet groups: sham-a purified diet control, gastrectomized-control, gastrectomized-isoflavone (0.2% isoflavone glycosides), gastrectomized-fructooligosaccharides (7.5% fructooligosaccharides), and isoflavone and fructooligosaccharides (0.2% isoflavone glycosides + 7.5% fructooligosaccharides). After 6 weeks, the rats were killed and biological samples were collected. In gastrectomized rats, fructooligosaccharides prevented femoral bone fragility, but isoflavone without fructooligosaccharides did not inhibit postgastrectomy osteopenia. Isoflavone and fructooligosaccharides exhibited a synergistic in the distal metaphyseal trabecular bone, indicated by peripheral quantitative computed tomography. Moreover, fructooligosaccharides increased calcium absorption and equol production from daidzein in gastrectomized rats. These results indicate that isoflavone alone did not inhibit postgastrectomy osteopenia, but the combination of isoflavone and fructooligosaccharides improved the inhibition of trabecular bone loss by increasing calcium absorption and equol production through fructooligosaccharides supplementation.
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Kimira Yoshifumi, Katsumata Shin-ichi, Suzuki Kazuharu, Ishimi Yoshiko, Ueno Tomomi, Uchiyama Shigeto, Adlercreutz Herman, Uehara Mariko
Biosci Biotechnol Biochem 76 ( 5 ) 1018 - 1021 2012
Language：English Publishing type：Research paper (scientific journal)
We compared the effects of the S-enantiomer and racemic forms of equol on bone using ovariectomized (OVX) mice. Femoral bone mineral density and bone strength decreased in the OVX mice, but not in OVX mice administered 0.5 mg/d S-equol. This, however, did not hold for racemic equol. Serum and urine S-equol concentrations were higher in the mice administered S-equol than in those administered racemic equol. These results suggest that the inhibitory effects of S-equol on bone fragility in OVX mice are greater than those of racemic equol.
DOI： 10.1271/bbb.110973