KIKUCHI Hidetomo



Assistant Professor

Homepage URL

Graduating School 【 display / non-display

  • 2000.04

    Tokyo University of Pharmacy and Life Science   Faculty of Pharmaceutical Science   Graduated

Campus Career 【 display / non-display

  • 2014.04

    Josai UniversityFaculty of Pharmaceutical Sciences   Department of Clinical Dietetics and Human Nutrition   Assistant Professor  

External Career 【 display / non-display

  • 2011.04

      Research Assistant  

Academic Society Affiliations 【 display / non-display

  • 2018.10

    Japan Society for Science Education

Field of expertise (Grants-in-aid for Scientific Research classification) 【 display / non-display

  • Biological pharmacy

Qualification acquired 【 display / non-display

  • Pharmacist


Papers 【 display / non-display

  • Differentiation induction of human breast cancer cells by arsenite in combination with tetrandrine

    Bowen Yu, Bo Yuan, Anna Kiyomi, Hidetomo Kikuchi, Hideki Hayashi, Xiaomei Hu, Mari Okazaki, Munetoshi Sugiura, Toshihiko Hirano, Xiaohua Pei, and Norio Takagi

    American Journal of Translational Research      [Refereed]

    Multiple Authorship


  • Chemopreventive and anticancer activity of flavonoids and its possibility for clinical use by combining with conventional chemotherapeutic agents

    Hidetomo Kikuchi, Bo Yuan, Xiaomei Hu, Mari Okazaki

    American Journal of Cancer Research ( e-Century Publishing Corporation )     [Refereed]

    Multiple Authorship


  • Enhanced cytotoxic effects of arsenite in combination with anthocyanidin compound, delphinidin, against a human leukemia cell line, HL-60

    Yuta Yoshino⁠, Bo Yuan, Saki Okusumia, Reiji Aoyama⁠, Ryo Murota, Hidetomo Kikuchi⁠, Norio Takagi⁠, Hiroo Toyoda⁠

    Chemico-Biological Interactions ( ELSEVIER )     [Refereed]

    Multiple Authorship

    Among five major anthocyanin compounds, delphinidin exhibited the most potent and selective cytocidal effect against HL-60, a trivalent arsenic (As(III))-resistant cell line. Co-treatment with delphinidin and As(III) resulted in the reduction of IC50 value for As(III) from 11.2 to 1.5 μM, which was considered as clinically achieved concentrations of As(III). The combination treatment strongly preferred to selectively enhance the cytotoxicity of As(III) against HL-60 cells rather than human peripheral blood mononuclear cells. The induction of apoptosis as evidenced by the increase of sub-G1 cells, DNA fragmentation, annexin V-positive cells and the activation of caspase-8, -9 and -3 was observed in HL-60 cells co-treated with As(III) and delphinidin. Similar to the activation pattern of caspases, a substantial decrease in the expression level of Bid along with the loss of mitochondrial membrane potential was also observed. These results suggested that the combination treatment triggered a convergence of the intrinsic and extrinsic pathways of apoptosis via the activation of caspase-8 and cleaved Bid. Delphinidin itself significantly decreased the intracellular GSH ([i]GSH) and nuclear factor-κB (NF-κB) binding activity, and further returned As(III)-triggered increment of [i]GSH and enhancement of NF-κB binding activity to control level. Additionally, buthionine sulfoximine, a GSH depletor; JSH-23, a NF-κB inhibitor, also mimicked the capacity of delphinidin to significantly induce the reduction of [i]GSH along with the potentiation of As(III) cytotoxicity in HL-60 cells. These observations suggested that delphinidin-induced sensitization of HL-60 cells to As(III) was caused by the reduction of [i]GSH, which was probably associated with the inhibitory effect of delphinidin on NF-κB binding activity. These findings further suggest that delphinidin-induced sensitization of HL-60 cells to As(III) may lead to dose reduction of As(III) in clinical application, and ultimately contribute to minimizing its side effects.

    DOI PubMed

  • Effects of tomato juice on the pharmacokinetics of CYP3A4-substrate drugs

    Atsuko Ohkubo, Tomomi Chida, Hidetomo Kikuchi, Tadashi Tsuda, Katsuyoshi Sunaga

    Asian Journal of Pharmaceutical Sciences ( Elsevier )     [Refereed]

    Multiple Authorship

    We previously demonstrated that tomato juice (TJ) contains potent mechanism-based inhibitor(s) of CYP3A4. In this study, we investigated the effects of TJ and grapefruit juice (GFJ) on the pharmacokinetics of the CYP3A4-substrate drugs, nifedipine (NFP) and midazolam (MDZ), in male Wistar rats. Oral administration of GFJ 90 min before the intraduodenal administration of NFP or MDZ increased the area under the concentration–time curves (AUCs) of NFP and MDZ by 32.4% and 89.4%, respectively. TJ increased MDZ blood concentrations and AUC after intraduodenal MDZ administration; however, it had no effect on NFP. When MDZ and NFP were intravenously administered, GFJ significantly increased the AUC of MDZ, but only slightly increased that of NFP. In contrast, TJ only slightly increased the AUC of MDZ. These results suggest that, similar to GFJ, TJ influences the pharmacokinetics of CYP3A4-substrate drugs; however, it may be a drug-dependent partial effect.


  • Rosehip inhibits xanthine oxidase activity and reduces serum urate levels in a mouse model of hyperuricemia

    Hidetomo Kikuchi, Satomi Kogure, Rie Arai, Kouki Saino, Atsuko Ohkubo, Tadashi Tsuda and Katsuyoshi Sunaga

    Biomedical Reports ( Spandidos Publications )     [Refereed]

    Multiple Authorship

    Rosehip, the fruit of Rosa canina L., has traditionally been used to treat urate metabolism disorders; however, its effects on such disorders have not been characterized in detail. Therefore, the present study investigated the effects of hot water, ethanol and ethyl acetate extracts of rosehip on xanthine oxidase (XO) activity in vitro. In addition, the serum urate lowering effects of the rosehip hot water extract in a mouse model of hyperuricemia (male ddY mice, which were intraperitoneally injected with potassium oxonate) were investigated. Furthermore, the influence of rosehip hot water extract on CYP3A4 activity, which is the most important drug‑metabolizing enzyme from a herb‑drug interaction perspective, was investigated. Rosehip extracts of hot water, ethanol and ethyl acetate inhibited XO activity [half maximal inhibitory concentration (IC50) values: 259.6±50.6, 242.5±46.2 and 1,462.8±544.2 µg/ml, respectively]. Furthermore, the administration of 1X rosehip hot water extract significantly reduced the levels of serum urate at 8 h, which was similar when compared with the administration of 1 mg/kg allopurinol. Rosehip hot water extract only marginally affected CYP3A4 activity (IC50 value, >1 mg/ml). These findings indicate that rosehip hot water extract may present as a functional food for individuals with a high urate level, and as a therapeutic reagent for hyperuricemic patients.

    DOI PubMed

display all >>

Review Papers 【 display / non-display

  • Development of the Database of Food-Drug Interaction and Contraindicated Food for Anticancer-Drugs

      25 ( 1 ) 13 - 18   2018

    Introduction and explanation (bulletin of university, research institution)   Multiple Authorship

Grant-in-Aid for Scientific Research 【 display / non-display

  • Grant-in-Aid for Scientific Research(C)

    Project Year: 2016.04  -  2019.03 


Social Contribution 【 display / non-display

  • 2019.02

  • 2019.02

  • 2017.07