Details of a Researcher

Papers - TODO Hiroaki

Division display >> ／ All the affair displays 1 - 172 of about 172

Effect of the barrier function of stratum corneum and viable epidermis and dermis on the skin concentration of topically applied chemicals Reviewed

Hiroaki Todo, Takeshi Oshizaka, Syuuhei Komatsu, Kenji Sugibayashi

The Journal of Toxicological Sciences 2025.02

Authorship：Lead author,　Corresponding author Language：English Publishing type：Research paper (scientific journal)

Effect of the barrier function of stratum corneum and viable epidermis and dermis on the skin concentration of topically applied chemicals Reviewed

Hiroaki Todo, Ibuki Narita, Chihiro Fujiwara, Hiroyuki Teramae, Takeshi Oshizaka, Shoko Itakura, Syuuhei Komatsu, Kozo Takayama, Kenji Sugibayashi

The Journal of Toxicological Sciences 2024.12

Authorship：Lead author,　Corresponding author Language：English Publishing type：Research paper (scientific journal)

経口マルチキナーゼ阻害薬スニチニブの味覚障害機序解明のための病理学的検討 Reviewed

藤堂真紀, 川野竜太郎, 日下卓万, 藤堂浩明, 坂田康彰, 田丸淳一, 佐伯俊昭

薬理と治療 52 ( 11 ) 1311 - 1317 2024.12

Language：Japanese Publishing type：Research paper (scientific journal)

Plasmid DNA Delivery into the Skin via Electroporation with a Depot-Type Electrode. Reviewed

Yoshida Y, Aoki M, Nagase K, Marubashi K, Kojima H, Itakura S, Komatsu S, Sugibayashi K, Todo H.

Pharmaceutics 16 ( 9 ) 2024.09

Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)

Characterization, stability, and skin application of astaxanthin particulates Reviewed

iyu Ai, Risa Kanai, Hiroaki Todo, Junki Tomita, Takashi Tanikawa & Yutaka Inoue

AAPS Open 9 2024.09

Language：English Publishing type：Research paper (scientific journal)

Study of the preparation, characterization, and solubility of lidocaine complexed with 5-sulfosalicylic acid dihydrate. Reviewed

Nomura M, Tomita J, Itakura S, Todo H, Kodama N, Inoue Y.

Drug Dev Ind Pharm 50 ( 7 ) 628 - 638 2024.07

Language：English Publishing type：Research paper (scientific journal)

Integrating mathematical approaches (IMAS): Novel methodology for predicting dermal absorption rates of chemicals under finite dose conditions. Reviewed

Kunita R, Nishijima T, Todo H, Miyazawa M.

J Toxicol Sci. 49 ( 5 ) 219 - 230 2024.07

Language：English Publishing type：Research paper (scientific journal)

Development of an Auraptene-Loaded Transdermal Formulation Using Non-ionic Sugar Ester Surfactants Reviewed International coauthorship

Flores KA, Okada A, Arce F Jr, See GL, Itakura S, Todo H, Sugibayashi K.

Chem Pharm Bull (Tokyo) 72 319 - 323 2024.06

Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)

Development of rice bran-derived nanoparticles with excellent anti-cancer activity and their application for peritoneal dissemination. Reviewed

Sasaki D, Suzuki H, Kusamori K, Itakura S, Todo H, Nishikawa M.

J Nanobiotechnology 22 2024.05

Language：English Publishing type：Research paper (scientific journal)

A Hollow Microneedle Equipped with a Micropillar for Improved Needle Insertion and Injection of Drug Solution Reviewed

Futaki M, Inamura K, Nishimura T, Niitsu T, Tojo T, Sugibayashi K, Todo H.

Pharm Res 41 ( 4 ) 819 - 831 2024.04

Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)

Characterization of Carbohydrates, Amino Acids, Viscosity, and Antioxidant Capacity in Rice Wines Made in Saitama, Japan, with Different Sake Rice Reviewed

Yutaka Inoue, Sae Ueda, Takashi Tanikawa, Aiko Sano, Ryuichiro Suzuki, Hiroaki Todo, Yuji Higuchi, Kenichi Akao

Foods 12 ( 21 ) 2023.11

Language：English Publishing type：Research paper (scientific journal)

Development of a depot formulation with an in situ non-lamellar liquid crystal-forming system with phospholipids Reviewed

Hiroaki Todo, Rina Niki, Akie Okada, Ibuki Narita, Kazuya Inamura, Ayu Ito, Shoko Itakura, Ichiro Hijikuro, Kenji Sugibayashi

Frontiers in Drug Delivery 3 2023.10

Authorship：Lead author,　Corresponding author Language：English Publishing type：Research paper (scientific journal)

Effects of Intradermal Administration Volume Using a Hollow Microneedle on the Pharmacokinetics of Fluorescein Isothiocyanate Dextran (M.W. 4,000). Reviewed

Mika Futaki, Kazuya Inamura, Miyu Hashimoto, Shunsaku Motegi, Shoko Itakura, Kenji Sugibayashi, Hiroaki Todo

Mika Futaki, Kazuya Inamura, Miyu Hashimoto, Shunsaku Motegi, Shoko Itakura, Kenji Sugibayashi, Hiroaki Todo Pharmaceutical research 40 ( 8 ) 1953 - 1963 2023.08

Authorship：Corresponding author Language：English

Evaluation of Skin Permeation Order of Cosmetic Active Compounds from Commercially Available Cosmetic Products with Different Dosage Forms Using a Three-Dimensional Cultured Human Skin Model Reviewed

Shohei SEKIGUCHI, Ibuki NARITA, Shoko ITAKURA, Hajime KOJIM, Kenji SUGIBAYASHI, Hiroaki TODO

Journal of Japanese Cosmetic Science Society 47 ( 3 ) 176 - 182 2023.06

Authorship：Corresponding author Language：English

Prediction of Critical Quality Attributes Based on the Numerical Simulation of Stress and Strain Distributions in Pharmaceutical Tablets Reviewed

Kozo Takayama, Shoko Itakura, Hiroaki Todo, Kenji Sugibayashi

Chemical and Pharmaceutical Bulletin 71 ( 6 ) 386 - 397 2023.06

Language：English

Design of an Ante‐enhancer with an Azone‐Mimic Structure using Ionic Liquid Reviewed

Takeshi Oshizaka, Mao Hayakawa, Mayu Uesaka, Kota Yoshizawa, Tomoyo Kamei, Issei Takeuchi, Kenji Mori, Shoko Itakura, Hiroaki Todo & Kenji Sugibayashi

Pharmaceutical Research 2023.04

Language：English Publishing type：Research paper (scientific journal)

Usefulness of direct intratumoral administration of doxorubicin hydrochloride with an electro-osmosis–assisted pump Reviewed

Ayu Ito, Shoko Itakura, Yuya Hasegawa, Miyu Hashimoto, Akie Okada, Mamoru Hirafuji, Hidenori Nakamura, Kenji Sugibayashi an, Hiroaki Todo

Front. Drug Deliv. ( 3 ) 2023.03

Authorship：Last author Language：English Publishing type：Research paper (scientific journal)

Gene knockdown in HaCaT cells by small interfering RNAs entrapped in grapefruit-derived extracellular vesicles using a microfluidic device. Reviewed

takura S, Shohji A, Amagai S, Kitamura M, Takayama K, Sugibayashi K, Todo H.

Sci Rep. 13 ( 1 ) 2023.02

Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)

Remote-controllable dosage management through a wearable iontophoretic patch utilizing a cell phone. Reviewed

Kenji Mori, Kotomi Yamazaki, Chihiro Takei, Takeshi Oshizaka, Issei Takeuchi, Kunio Miyaji, Hiroaki Todo, Shoko Itakura, Kenji Sugibayashi

Journal of controlled release 355 1 - 6 2023.01

Language：English

A step-by-step approach for assessing acute oral toxicity without animal testing for additives of quasi-drugs and cosmetic ingredients. Reviewed

Hajime Kojima , Tokio Nakada , Akiko Yagami , Hiroaki Todo , Jihei Nishimura , Mio Yagi , Keiko Yamamoto , Mariko Sugiyama , Yoshiaki Ikarashi , Hitoshi Sakaguchi , Masahiko Yamaguchi , Morihiko Hirota , Sakiko Aizawa , Shota Nakagawa , Shigenobu Hagino , Masato Hatao

Current research in toxicology 4 2023.01

Language：English

Verification of nanoparticle formation, skin permeation, and apoptosis using nobiletin as a methoxyflavonoid derivative Reviewed

Yutaka Inoue, Moe Ishizawa, Shoko Itakura, Takashi Tanikawa, Hiroaki Todo

AAPS Open 8 2022.11

Language：English Publishing type：Research paper (scientific journal)

A Mathematical Approach Using Strat-M® to Predict the Percutaneous Absorption of Chemicals under Finite Dose Conditions. Reviewed

Kunita R, Nishijima T, Todo H, Sugibayashi K, Sakaguchi H.

Pharmaceutics 14 ( 7 ) 2022.06

Language：English Publishing type：Research paper (scientific journal)

DOI： 10.3390/pharmaceutics14071370.

Preparation, Characterization, Solubility, and Antioxidant Capacity of Ellagic Acid-Urea Complex Reviewed

Sakurai H, Suzuki M, Itakura S, Todo H, Arce F Jr, See GL, Tanikawa T, Inoue Y.

Materials 15 ( 8 ) 2022.04

Language：English

DOI： 10.3390/ma15082836.

Self-Assembled Structure of α-Isostearyl Glyceryl Ether Affects Skin Permeability-a Lamellar with 70-nm Spaces and L3 Phase Enhanced the Transdermal Delivery of a Hydrophilic Model Drug Reviewed

Tomohiko Sano, Akie Okada, Kazunori Kawasaki, Takuji Kume, Minoru Fukui, Hiroaki Todo, Kenji Sugibayashi

AAPS PharmSciTech 23 ( 8 ) 2022.04

Language：English Publishing type：Research paper (scientific journal)

A Pilot Study of Transdermal Application of Diphenhydramine to the Nasal Ala in Patients with Allergic Rhinitis and Asthma. Reviewed

Kamimura M, Inui T, Mouri A, Todo H, Sugibayashi K, Asano K.

Tokai J Exp Clin Med. 47 ( 4 ) 2022.04

Language：English Publishing type：Research paper (scientific journal)

A step-by-step approach for assessing acute oral toxicity without animal testing for additives of quasi-drugs and cosmetic ingredients. Reviewed

Kojima H, Nakada T, Yagami A, Todo H, Nishimura J, Yagi M, Yamamoto K, Sugiyama M, Ikarashi Y, Sakaguchi H, Yamaguchi M, Hirota M, Aizawa S, Nakagawa S, Hagino S, Hatao M.

Curr Res Toxicol. 4 2022.04

Language：English Publishing type：Research paper (scientific journal)

Remote-controllable dosage management through a wearable iontophoretic patch utilizing a cell phone Reviewed

Mori K, Yamazaki K, Takei C, Oshizaka T, Takeuchi I, Miyaji K, Todo H, Itakura S, Sugibayashi K.

Journal of controlled release 355 2022.04

Language：English Publishing type：Research paper (scientific journal)

Intraperitoneal administration of nanoparticles containing tocopheryl succinate prevents peritoneal dissemination Reviewed

Hama S, Nishi T, Isono E, Itakura S, Yoshikawa Y, Nishimoto A, Suzuki S, Kirimura N, Todo H, Kogure K.

Cancer Sci 113 ( 5 ) 1779 - 1788 2022.04

Language：English

DOI： 10.1111/cas.15321

Estimation of skin absorption of chemicals with Fick's diffusion model Reviewed

Hiroaki Todo

Nihon Yakurigaku Zasshi 157 ( 5 ) 335 - 339 2022.04

Authorship：Lead author,　Corresponding author Language：Japanese Publishing type：Research paper (scientific journal)

Development of Self-Administered Formulation to Improve the Bioavailability of Leuprorelin Acetate. Reviewed

Okada A, Niki R, Inoue Y, Tomita J, Todo H, Itakura S, Sugibayashi K.

Pharmaceutics 14 ( 4 ) 2022.04

Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)

DOI： 10.3390/pharmaceutics14040785.

Enhancement of skin permeation of fluorescein isothiocyanate-dextran 4 kDa (FD4) and insulin by thermalporation Reviewed

Ono N, Iibuchi T, Todo H, Itakura S, Adachi H, Sugibayashi K.

Eur J Pharm Sci . 2022.03

Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)

DOI： 10.1016/j.ejps.2021.106096

Estimation of skin absorption of chemicals with Fick's diffusion model

Hiroaki Todo

Nihon yakurigaku zasshi 157 ( 5 ) 335 - 339 2022.01

Development of nanoparticles derived from corn as mass producible bionanoparticles with anticancer activity. Reviewed

Daisuke Sasaki, Kosuke Kusamori, Yukiya Takayama, Shoko Itakura, Hiroaki Todo, Makiya Nishikawa

Scientific reports 2021.11

Language：English Publishing type：Research paper (scientific journal)

Validation study of a new reconstructed human epidermis model EPiTRI for in vitro skin irritation test according to OECD guidelines. Reviewed International coauthorship

Chih-Ching Liao, Cheng-Yi Wu, Meng-Hsueh Lin, Feng-Koo Hsieh, Lih-Tao Hsu, Shiun-Yin Chang, Kuan-Jen Chen, Hui-Ting Huang, Hui-Chun Hsu, Chiu-Hsing Lin, Pei-Ju Lin, Huey-Min Lai, Hajime Kojima, Hiroaki Todo, Sung-Jan Lin, Jih-Heng Li, Wannhsin Chen

Toxicology in vitro 2021.09

Language：English Publishing type：Research paper (scientific journal)

Effect of Rubbing Application on the Skin Permeation of Active Ingredients from Lotion and Cream Reviewed International journal

Abe A, Suzuki H, Amagai S, Saito M, Itakura S, Todo H, Sugibayashi K.

Chem Pharm Bull 69 ( 8 ) 806 - 810 2021.04

Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)

Design and Optimization of Scored Tablets with Concave Surface and Application of Bayesian Estimation for Solving Scaleup Problem. Reviewed

Hiroki Katayama, Yoshiharu Maeda, Tsubasa Sato, Asami Mogi, Shoko Itakura, Hiroaki Todo, Kenji Sugibayashi, Kozo Takayama

Chemical & pharmaceutical bulletin 69 ( 11 ) 2021.04

Language：English Publishing type：Research paper (scientific journal)

Effect of Iontophoresis on the Intradermal Migration Rate of Medium Molecular Weight Drugs Reviewed International journal

Kenji Sugibayashi, Mika Futaki , Miyu Hashimoto, Asuka Fukuhara , Kengo Matsumoto, Takeshi Oshizaka , Shoko Itakura , Hiroaki Todo

Chem Pharm Bull (Tokyo) 69 639 - 645 2021.04

Language：English Publishing type：Research paper (scientific journal)

Improvement of Skin Permeation of Caffeine, a Hydrophilic Drug, by the Application of Water Droplets Provided by a Novel Humidifier Device. Reviewed International journal

Todo H, Hasegawa Y, Okada A, Itakura S, Sugibayashi K.

Chem Pharm Bull 69 ( 8 ) 674 - 680 2021.04

Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)

ナノ粒子の皮膚浸透性と安全性

木村恵理子 , 藤堂浩明 , 杉林堅次

薬学雑誌 2021.03

Application of diphenydramine ointment to the eyelids for allergic conjunctivitis Reviewed International journal

Mitsuhiro Kamimura, Takako Ohnish, Hisao Terada, Atsuto Mouri, Takahiro Naoi, Hiroaki Todo, Kenji Sugibayashi

IBEROAMERICAN JOURNAL OF MEDICINE 44 - 50 2021.01

Language：English Publishing type：Research paper (scientific journal)

Enhancement of Skin Permeation of a Hydrophilic Drug from Acryl-Based Pressure-Sensitive Adhesive Tape Reviewed International journal

Suzuki, T., Aoki, T., Saito, M., Hijikuro, I., Itakura, S., Todo, H., Sugibayashi, K.

Pharmaceutical Research 2021.01

Language：English Publishing type：Research paper (scientific journal)

A Lipid-Based Depot Formulation with a Novel Non-lamellar Liquid Crystal Forming Lipid Reviewed International journal

Akie Okada, Hiroaki Todo , Shoko Itakura , Ichiro Hijikuro, Kenji Sugibayashi

Pharmaceutical Research 2021.01

Language：English Publishing type：Research paper (scientific journal)

Latent Structure Analysis of Wet-Granulation Tableting Process Based on Structural Equation Modeling Reviewed

Katayama H, Itakura S, Todo H, Sugibayashi K, Takayama K.

Chem Pharm Bull 69 ( 7 ) 674 - 680 2021.01

Language：English Publishing type：Research paper (scientific journal)

各種市販ミノキシジル製剤の安全性・有効性に及ぼす容器の種類の影響 Reviewed

藤堂浩明, 齋藤美幸, 鈴木宏宙, 井上裕, 高山幸三, 杉林堅次

薬剤学: 生命とくすり 81 ( 1 ) 111 - 120 2021.01

Language：Japanese Publishing type：Research paper (scientific journal)

感染から治癒過程を表現する「感染症数理疫学」と吸収から消失過程を表現する「薬物動態学」の類似点と相違点 Reviewed

杉林堅次, 森健二, 押坂勇志, 武井千弥, 藤堂浩明, 板倉祥子, 高山幸三

薬剤学: 生命とくすり 80 ( 6 ) 322 - 329 2020.11

Language：Japanese Publishing type：Research paper (scientific journal)

Prolonged Distribution of Tranilast in the Eyes after Topical Application onto Eyelid Skin Reviewed International journal

See, G.L., Florencio Arce, F.A., Itakura, S., Todo, H., Sugibayashi, K.

Chemical and Pharmaceutical Bulletin 2020.04

Language：English Publishing type：Research paper (scientific journal)

Development of spray formulations applied to the oral mucosa using non-lamellar liquid crystal-forming lipids Reviewed International journal

Sugibayashi, K., Yamamoto, N., Itakura, S., Okada, A., Hijikuro, I., Todo, H.

Chemical and Pharmaceutical Bulletin 2020.04

Language：English Publishing type：Research paper (scientific journal)

Enhanced nose-to-brain delivery of tranilast using liquid crystal formulations Reviewed International journal

See, G.L., Arce, F., Dahlizar, S., Okada, A., Fadli, M.F.B.M., Hijikuro, I., Itakura, S., Katakura, M., Todo, H., Sugibayashi, K.

Journal of Controlled Release 2020.04

Language：English Publishing type：Research paper (scientific journal)

Effect of rubbing on the distribution of topically applied drugs into the hair follicles Reviewed International journal

Abe, A., Suzuki, H., Saito, M., Todo, H., Sugibayashi, K.

Chemical and Pharmaceutical Bulletin 2020.04

Language：English Publishing type：Research paper (scientific journal)

Physical properties of an ionic liquid composed of two water-soluble vitamins and enhanced skin permeation of both vitamins Reviewed International journal

Sugibayashi, K., Yoshida, Y., Suzuki, R., Yoshizawa, K., Mori, K., Itakura, S., Takayama, K., Todo, H.

Pharmaceutics 2020.04

Language：English Publishing type：Research paper (scientific journal)

Prediction of skin permeation and concentration of rhododendrol applied as finite dose from complex cosmetic vehicles Reviewed International journal

Arce, F., Asano, N., See, G.L., Oshizaka, T., Itakura, S., Todo, H., Sugibayashi, K.

International Journal of Pharmaceutics 2020.04

Language：English Publishing type：Research paper (scientific journal)

Controlled release of a model hydrophilic high molecular weight compound from injectable non-lamellar liquid crystal formulations containing different types of phospholipids Reviewed International journal

Okada, A., Todo, H., Hijikuro, I., Itakura, S., Sugibayashi, K.

International Journal of Pharmaceutics 2020.04

Language：English Publishing type：Research paper (scientific journal)

Usefulness of artificial membrane, strat-M®, in the assessment of drug permeation from complex vehicles in finite dose conditions Reviewed International journal

Arce, F.J., Asano, N., See, G.L., Itakura, S., Todo, H., Sugibayashi, K.

Pharmaceutics 2020.04

Language：English Publishing type：Research paper (scientific journal)

Pharmacokinetics and tissue distribution of pilocarpine after eyelid skin application to rats Reviewed International journal

1. Gerard Lee See, Ayano Sagesaka, Hiroaki Todo, KonstantyWierzba, KenjiSugibayashi,

J. Pharm. Sci. 108 ( 9 ) 2942 - 2948 2019.04

Language：English Publishing type：Research paper (scientific journal)

Halal cosmetics: A review on ingredients, production, and testing methods Reviewed International journal

Sugibayashi, K., Yusuf, E., Todo, H., Dahlizar, S., Sakdiset, P., Arce, F.J., See, G.L.

Cosmetics 2019.04

Language：English Publishing type：Research paper (scientific journal)

無限用量にて適用した種々剤形からのイソプロピルメチルフェノールの皮膚透過性と皮膚内濃度の関係について-ヒト長期投与(安全性)試験の用量設定法ガイドライン策定のための検証結果その1- Reviewed

藤堂浩明, 足立浩章, 足立浩章, 今井教安, 今井教安, 上中麻規子, 上中麻規子, 内田崇志, 内田崇志, 内田崇志, 大谷道輝, 大谷道輝, 澤田美月, 澤田美月, 成田昌稔, 成田昌稔, 西島貴史, 西島貴史, 野村宜史, 野村宜史, 宮坂美行, 宮坂美行, 畑尾正人, 増永卓司, 増永卓司, 山口雅彦, 山口雅彦, 佐々齊, 佐々齊, 知久真巳, 知久真巳, 川田裕三, 川田裕三, 古屋律子, 古屋律子, 藤井まき子,

日本香粧品学会誌 43 ( 2 ) 2019.04

Language：Japanese Publishing type：Research paper (scientific journal)

Effects of layered application on the skin permeation of a cosmetics active component, rhododendrol Reviewed

3. Florencio Villester Arce, Jr., Narumi Asano, Keita Yamashita, Ayaka Oda, Takashi Uchida, Tomohiko Sano, Hiroaki Todo, Kenji Sugibayashi,

J. Toxicol. Sci. 44 1 - 11 2019.04

Language：English Publishing type：Research paper (scientific journal)

Potential of biocompatible polymeric ultra-thin films, nanosheets, as topical and transdermal drug delivery devices Reviewed International journal

2. Tomomi Hatanaka、Takanori　Saito, Takaaki　Fukushima, Hiroaki Todo, Kenji Sugibayashi, Soichi Umehara, Tomoharu Takeuchi, Yosuke Okamura,

Int. J. Pharm 565 41 - 49 2019.04

Language：English Publishing type：Research paper (scientific journal)

Effects of Temperature and Humidity on the Skin Permeation of Hydrophilic and Hydrophobic Drugs Reviewed International journal

Iikura, H., Uchida, K., Ogawa-Fuse, C., Bito, K., Naitou, S., Hosokawa, M., Uchida, T., Sano, T., Todo, H., Sugibayashi, K.

AAPS PharmSciTech 2019.04

Language：English Publishing type：Research paper (scientific journal)

Prediction of tablet characteristics based on sparse modeling for residual stresses simulated by the finite element method incorporating Drucker-Prager cap model Reviewed International journal

Takayama, K., Sato, T., Sato, K., Todo, H., Obata, Y., Sugibayashi, K.

Journal of Drug Delivery Science and Technology 2019.04

Language：English Publishing type：Research paper (scientific journal)

無限用量にて適用した種々剤形からのカフェインの皮膚透過性と皮膚内濃度の関係について-ヒト長期投与(安全性)試験の用量設定法ガイドライン策定のための検証結果その2- Reviewed

藤堂浩明, 藤堂浩明, 足立浩章, 足立浩章, 今井教安, 今井教安, 上中麻規子, 上中麻規子, 内田崇志, 内田崇志, 内田崇志, 大谷道輝, 大谷道輝, 澤田美月, 澤田美月, 成田昌稔, 成田昌稔, 西島貴史, 西島貴史, 野村宜史, 野村宜史, 宮坂美行, 宮坂美行, 畑尾正人, 増永卓司, 増永卓司, 山口雅彦, 山口雅彦, 佐々齊, 佐々齊, 知久真巳, 知久真巳, 川田裕三, 川田裕三, 古屋律子, 古屋律子, 藤井まき子, 藤井まき子

日本香粧品学会誌 43 ( 3 ) 2019.04

Language：Japanese Publishing type：Research paper (scientific journal)

Prediction of skin permeation of flurbiprofen from near ester oils and their o/w emulsions Reviewed

Chem. Pharm. Bull. 66 959 - 966 2018.04

Language：English Publishing type：Research paper (scientific journal)

Iontophoresis-aided drug delivery into the eyeball via eyelid skin Reviewed International journal

See G.L., Sagesaka A., Torizuka H., Todo H., Sugibayashi K

J. Drug Del. Sci. Technol., 47 380 - 385 2018.04

Language：English Publishing type：Research paper (scientific journal)

Comparative assessment of 24-hr primary skin irritation test and human patch test data with in vitro skin irritation tests according to OECD TG439 (for quasi-drugs in Japan) Reviewed

Mariko Sugiyama, Masaharu Akita, Nathalie Alépée, Miyuki Fujishiro, Shigenobu Hagino, Yuki Handa, Hidefumi Ikeda, Noriyasu Imai, Setsuko Jitsukawa, Masakazu Katoh, Koji Kurihara, Daiki Kyotani, Shigeyuki Nomura, Yuko Okamoto, Hidenobu Okumura, Takashi Omori, Kenji Sugibayashi, Hiroaki Todo, Akemi Toyoda, Yasuo Ohno,

J. Toxicol. Sci., 43 751 - 768 2018.04

Language：English Publishing type：Research paper (scientific journal)

Combined use of N-palmitoyl-glycine-histidine gel and several penetration enhancers on the skin permeation and concentration of metronidazole, Reviewed International journal

14. Sabrina Dahlizar, Mika Futaki, Akie Okada, Chihiro Yatomi, Hiroaki Todo, Kenji Sugibayashi

Pharmaceutics 43 2018.04

Language：English Publishing type：Research paper (scientific journal)

Optimization of Premix Powders for Tableting Use Reviewed

Chem. Pharm. Bull., 66 748 - 756 2018.04

Language：English Publishing type：Research paper (scientific journal)

Design of topically applied gel-spray formulation of ivermectin using a novel low molecular gelling agent, palmitoyl-glycine-histidine, to treat scabie Reviewed

Chem. Pharm. Bull 66 327 - 333 2018.04

Language：English Publishing type：Research paper (scientific journal)

Establishment of an evaluation method to detect drug distribution in hair follicles Reviewed International journal

5. Akinari Abe, Miyuki Saito, Wesam R. Kadhum, Hiroaki Todo, Kenji Sugibayashi,

Int. J. Pharm. 542 27 - 35 2018.04

Language：English Publishing type：Research paper (scientific journal)

Selection of phospholipids to design liposome preparations with high skin penetration-enhancing effects, Reviewed International journal

Pajaree Sakdiset, Akie Okada, Hiroaki Todo, Kenji Sugibayashi,

J. Drug Del. Sci. Tech. 44 58 - 64 2018.04

Language：English Publishing type：Research paper (scientific journal)

Development of drug-in-adhesive patch with a honeycomb film as a backing layer Reviewed

Chem. Pharm. Bull, 66 748 - 756 2018.04

Language：English Publishing type：Research paper (scientific journal)

Optimization of premix powders for tableting use Reviewed

Hiroaki Todo, Kazuki Sato, Kozo Takayama, Kenji Sugibayashi

Chemical and Pharmaceutical Bulletin 66 ( 7 ) 748 - 756 2018.04

Language：English Publishing type：Research paper (scientific journal)

Purification of isopropyl fatty acids markedly changed the skin permeation of a model hydrophilic chemical Reviewed

Dai Qi, Hiroaki Todo, Kenji Sugibayashi

Journal of Drug Delivery Science and Technology 39 423 - 427 2017.06

Language：English Publishing type：Research paper (scientific journal)

天然の無機物系ナノ粒子-火山灰中のナノ粒子とその安全性- Reviewed

黒田章裕, 杉林堅次, 藤堂浩明, 伊藤公紀, 雨宮隆, 安部隆, 宮城磯治

J.Soc.Cosmet.Chem.Jpn. 51 318 - 325 2017.04

Language：English Publishing type：Research paper (scientific journal)

Potential of Stratum Corneum Lipid Liposomes for Screening of Chemical Skin Penetration Enhancers, Reviewed

22. Sakdiset P, Todo H, Sugibayashi K,

Chem Pharm Bull, 65 776 - 783 2017.04

Language：English Publishing type：Research paper (scientific journal) Publisher：日本薬学会

Safety evaluation of dermal exposure to phthalates: Metabolism-dependent percutaneous absorption Reviewed

Toxicol Appl Pharmacol 328 10 - 17 2017.04

Language：English Publishing type：Research paper (scientific journal)

Eyelid skin as a potential site for drug delivery to conjunctiva and ocular tissues Reviewed International journal

20. See GL, Sagesaka A, Sugasawa S, Todo H, Sugibayashi K

533 198 - 205 2017.04

Language：English Publishing type：Research paper (scientific journal) Publisher：Int. J. Pharm

Prediction of Dissolution Data Integrated in Tablet Database Using Four-Layered Artificial Neural Networks Reviewed

Chem Pharm Bull 65 967 - 972 2017.04

Language：English Publishing type：Research paper (scientific journal)

Transdermal permeation of drugs in various animal species Invited Reviewed International journal

Hiroaki　Todo

Pharmaceutics 9 2017.04

Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)

Development and optimization of orally and topically applied liquid crystal drug formulations Reviewed

Wesam R. Kadhum, Tomoki Hada, Ichiro Hijikuro, Hiroaki Todo, Kenji Sugibayashi

J Oleo Sci 2017.01

Language：English Publishing type：Research paper (scientific journal)

High-Throughput Screening of Potential Skin Penetration-Enhancers Using Stratum Corneum Lipid Liposomes: Preliminary Evaluation for Different Concentrations of Ethanol. Reviewed

Sakdiset P, Kitao Y, Todo H, Sugibayashi K.

J Pharm 2017.01

Authorship：Second author Language：English Publishing type：Research paper (scientific journal)

In Silico Estimation of Skin Concentration of Dermally Metabolized Chemicals Reviewed

Tomomi Hatanaka, Saki Yamamoto, Mayuko Kamei, Wesam R Kadhum, Hiroaki Todo and Kenji Sugibayashi

International Journal of Pharmaceutical Sciences and Developmental Research 2017.01

Language：English Publishing type：Research paper (scientific journal)

A useful technique using imaging mass spectrometry for detecting the skin distribution of topically applied lidocaine Reviewed

Shosho Kijima, Hiroaki Todo, Yumi Matsumoto, Ryosuke Masaki, Wesam R. Kadhum, Kenji Sugibayashi

Journal of Drug Delivery Science and Technology 33 157 - 163 2016.06

Language：English Publishing type：Research paper (scientific journal)

Effect of Combination of Low-Frequency Sonophoresis or Electroporation with Iontophoresis on the Mannitol Flux or Electroosmosis through Excised Skin Reviewed

Tokumoto S, Higo N, Todo H, Sugibayashi K

Biol Pharm Bull 2016.01

Authorship：Second author Language：English Publishing type：Research paper (scientific journal)

Risk assessment of skin lightening cosmetics containing hydroquinone., Regul Toxicol Pharmacol. Reviewed

Matsumoto M, Todo H, Akiyama T, Hirata-Koizumi M, Sugibayashi K, Ikarashi Y, Ono A, Hirose A, Yokoyama K.

Regul Toxicol Pharmacol. 2016.01

Language：English Publishing type：Research paper (scientific journal)

Effect of Esters on the Permeation of Chemicals with Different Polarities through Synthetic Artificial Membranes Using a High-Throughput Diffusion Cell Array. Reviewed

Uchida T, Nishioka K, Motoki A, Yakumaru M, Sano T, Todo H, Sugibayashi K

Chem Pharm Bull (Tokyo) 2016.01

Language：English Publishing type：Research paper (scientific journal)

Contribution of the Hair Follicular Pathway to Total Skin Permeation of Topically Applied and Exposed Chemicals Reviewed

Mohd F, Todo H, Yoshimoto M, Yusuf E, Sugibayashi K

Pharmaceutics 2016.01

Authorship：Second author Language：English Publishing type：Research paper (scientific journal)

In Vitro Permeation and Skin Retention of α-Mangostin Proniosome, Reviewed

Chin GS, Todo H, Kadhum WR, Hamid MA, Sugibayashi K

Chem Pharm Bull (Tokyo) 2016.01

Authorship：Second author Language：English Publishing type：Research paper (scientific journal)

A Novel Chemical Enhancer Approach for Transdermal Drug Delivery with C17-Monoglycerol Ester Liquid Crystal-forming Lipid Reviewed

Kadhum WR, Sekiguchi S, Hijikuro I, Todo H, Sugibayashi K.

J Oleo Sci 2016.01

Authorship：Second author Language：English Publishing type：Research paper (scientific journal)

Evaluation of a Silicone Membrane as an Alternative to Human Skin for Determining Skin Permeation Parameters of Chemical Compounds Reviewed

Uchida T, Yakumaru M, Nishioka K, Higashi Y, Sano T, Todo H, Sugibayashi K

Chem Pharm Bull (Tokyo) 2016.01

Language：English Publishing type：Research paper (scientific journal)

Usefulness of liquid-crystal oral formulations to enhance the bioavailability and skin tissue targeting of p-amino benzoic acid as a model compound Reviewed

Kadhum WR, Oshizaka T, Ichiro H, Todo H, Sugibayashi K

Eur J Pharm Sci 2016.01

Language：English Publishing type：Research paper (scientific journal)

In Silico Estimation of Skin Concentration Following the Dermal Exposure to Chemicals. Reviewed

Tomomi Hatanaka, Shun Yoshida, Wesam R Kadhum, Hiroaki Todo, Kenji Sugibayashi

Pharmaceutical research 32 ( 12 ) 3965 - 3974 2015.12

Language：English Publishing type：Research paper (scientific journal)

A new approach for evaluating cellular damage by measuring calcium level with Fura-2 acetoxymethyl ester (Fura-2-AM) Reviewed

Wesam R. Kadhum, Manami Mitsuyama, Toru Tanaka, Hiroaki Todo, Kazuhiko, Juni, Kenji Sugibayashi,

Alternatives to Animal Testing and EXperimentation 2015.10

Language：English Publishing type：Research paper (scientific journal)

In Silico Estimation of Skin Concentration Following the Dermal Exposure to Chemicals Reviewed

Hatanaka T, Yoshida S, Kadhum WR, Todo H, Sugibayashi K

Pharm Res 2015.07

Language：English Publishing type：Research paper (scientific journal)

Prediction of skin permeation by chemical compounds using the artificial membrane, Strat-M™. Reviewed

Takashi Uchida, Wesam R Kadhum, Sayumi Kanai, Hiroaki Todo, Takeshi Oshizaka, Kenji Sugibayashi

European journal of pharmaceutical sciences 67 113 - 118 2015.01

Language：English Publishing type：Research paper (scientific journal)

Determination of permeation pathways of clindamycin phosphate into the skin, Reviewed

Fadli A., Mohammad Nizam A. G.,Ibrahim A., Kavitha M., Jiyauddin K., Samer, A. D., S. Budiasih, M. Kaleemullah ,Jawad A., Rasha S., Todo H., Sugibayashi K., Eddy Y.

Am. J. PharmTech. Res., 2015.01

Language：English Publishing type：Research paper (scientific journal)

Potential of imaging analysis in establishing skin concentration-distance profiles for topically applied FITC-dextran 4 kDa Reviewed

Shosho Kijima, Ryosuke Masaki, Wesam R. Kadhum, Hiroaki Todo, Tomomi Hatanaka, Kenji Sugibayashi,

ADMET and DMPK 2015.01

Language：English Publishing type：Research paper (scientific journal)

Molecular mechanisms of action of different concentrations of ethanol in water on ordered structures of intercellular lipids and soft keratin in the stratum corneum Reviewed

1. D. Horita, H., I. Hatta, M Yoshimoto, Y. Kitao, H. Todo, K. Sugibayashi

BBA – Biomembranes 2015

Language：English Publishing type：Research paper (scientific journal)

Formulation of Tetracycline nano-emulsion Reviewed

Fadli A., Nizam A. G., Ibrahim A. , Nurulnadiah A. K., Jiyauddin K., Samer A. D., S. Budiasih, M. Kaleemullah, Jawad A., Rasha S., Todo H., Sugibayashi K. and Eddy Y.

WJPR 2015

Language：English Publishing type：Research paper (scientific journal)

Determination of permeation of pathway of Gentamicin into pig’s ear skin Reviewed

Fadli A., Jiyauddin K., Siti Nur S. J., Samer A. D., S. Budiasih, Jawad A., M. Kaleemullah, Rasha S., Mohammad Nizam A. G., Ibrahim A., Todo H., Sugibayashi K. and Eddy Y.

IRJP 2015

Language：English Publishing type：Research paper (scientific journal)

Estimation of skin concentrations of topically applied lidocaine at each depth profile.

Oshizaka Takeshi, Kikuchi Keisuke, Kadhum Wesam R, Todo Hiroaki, Hatanaka Tomomi, Wierzba Konstanty, Sugibayashi Kenji

Int J Pharm 475 ( 1-2 ) 292 - 297 2014.11

Language：English Publishing type：Research paper (scientific journal)

Skin concentrations of topically administered compounds need to be considered in order to evaluate their efficacies and toxicities. This study investigated the relationship between the skin permeation and concentrations of compounds, and also predicted the skin concentrations of these compounds using their permeation parameters. Full-thickness skin or stripped skin from pig ears was set on a vertical-type diffusion cell, and lidocaine (LID) solution was applied to the stratum corneum (SC) in order to determine in vitro skin permeability. Permeation parameters were obtained based on Fick's second law of diffusion. LID concentrations at each depth of the SC were measured using tape-stripping. Concentration-depth profiles were obtained from viable epidermis and dermis (VED) by analyzing horizontal sections. The corresponding skin concentration at each depth was calculated based on Fick's law using permeation parameters and then compared with the observed value. The steady state LID concentrations decreased linearly as the site became deeper in SC or VED. The calculated concentration-depth profiles of the SC and VED were almost identical to the observed profiles. The compound concentration at each depth could be easily predicted in the skin using diffusion equations and skin permeation data. Thus, this method was considered to be useful for promoting the efficient preparation of topically applied drugs and cosmetics.

DOI： 10.1016/j.ijpharm.2014.08.046

PubMed

Effect of liquid crystals with cyclodextrin on the bioavailability of a poorly water-soluble compound, diosgenin, after its oral administration to rats.

Okawara Masaki, Hashimoto Fumie, Todo Hiroaki, Sugibayashi Kenji, Tokudome Yoshihiro

Int J Pharm 472 ( 1-2 ) 257 - 261 2014.09

Language：English Publishing type：Research paper (scientific journal)

Diosgenin, found in wild yam (Dioscorea villosa), has been shown to ameliorate diabetes and hyperlipidemia, increase cell proliferation in a human 3D skin model, and inhibits melanin production in B16 melanoma cells. It is also an active element in cosmeceutical and dietary supplements. Although the bioavailability of diosgenin is low due to its poor solubility and intestinal permeability, it was subsequently improved using a beta-cyclodextrin (beta-CD) inclusion complex. Recently liquid crystals (LCs) were shown to enhance the bioavailability of poorly water-soluble drugs. The purpose in the present study was to prepare diosgenin LCs and investigate the interaction between LC and beta-CD in order to improve its bioavailability of diosgenin. Crystallinity and particle diameters of LCs in water were determined by small angle X-ray scattering (SAXS) and Zetasizer. Pharmacokinetic parameters were calculated using the plasma content of diosgenin after its oral administration to Wistar rats. Regarding the formation of glyceryl monooleate (GMO) and phytantriol (PHY) LC, SAXS patterns showed the hexagonal and cubic phases, respectively. Bioavailability was significantly enhanced after oral administration of LCs prepared by GMO than after diosgenin alone. The bioavailability was further improved with the combination of LC and beta-CD than LC and water.

DOI： 10.1016/j.ijpharm.2014.06.032

PubMed

Effect of emulsification on the skin permeation and UV protection of catechin. Reviewed

Sachie Yoshino, Tomoaki Mitoma, Keiko Tsuruta, Hiroaki Todo, Kenji Sugibayashi

Pharmaceutical development and technology 19 ( 4 ) 395 - 400 2014.06

Language：English Publishing type：Research paper (scientific journal)

Effect of emulsification on the skin permeation and UV protection of catechin.

Yoshino Sachie, Mitoma Tomoaki, Tsuruta Keiko, Todo Hiroaki, Sugibayashi Kenji

Pharm Dev Technol 19 ( 4 ) 395 - 400 2014.06

Language：English Publishing type：Research paper (scientific journal)

An anti-aging effect may be obtained by skin application of tea catechins (Camellia sinensis) since they have high ultraviolet (UV)-protection activity. In this study, the skin permeation of catechin (C), epicatechin (EC), epigallocatechin (EGC), epicatechin gallate (ECg) and epigallocatechin gallate (EGCg) was determined and compared, and the effect of emulsification on the skin permeation of C was measured. The UV-protective effect of C was also determined. The in vitro skin permeability of each catechin derivative was determined using side-by-side diffusion of cells. The UV-protective effect of C was determined by applying different concentrations of C to the solution or emulsion on a three-dimensional cultured human skin model or normal human epidermal keratinocytes with UV-irradiation. ECg and EGCg with gallate groups showed lower skin permeability than C, EC and EGC without gallate groups, suggesting that the skin permeability of catechin derivatives may be dependent on the existence of a gallate group. Interestingly, the skin permeation of C was increased by an o/w emulsification. In addition, the C emulsion showed a significantly higher UV-protective effect by C than that with its aqueous solution. These results suggest that the o/w emulsion of catechin derivatives is probably useful as a cosmetic formulation with anti-aging efficacy.

DOI： 10.3109/10837450.2013.788512

PubMed

Safety prediction of topically exposed biocides using permeability coefficients and the desquamation rate at the stratum corneum.

Sugino Masahiro, Todo Hiroaki, Suzuki Takamasa, Nakada Keiichi, Tsuji Kiyomi, Tokunaga Hiroshi, Jinno Hideto, Sugibayashi Kenji

J Toxicol Sci 39 ( 3 ) 475 - 485 2014.06

Language：English Publishing type：Research paper (scientific journal)

Advances in the synthesis and utilization of new chemical compounds have led to improvements in our daily lives. However, new chemicals may be both beneficial and toxic. Thus, exposure to these new compounds should be restricted in an attempt to limit their potential toxicities. We predicted the safety of three biocides (p-cresol, diazinon and resmethrin) by comparing their skin permeability coefficients and desquamation rate (the counter flux of permeability coefficient for chemical compounds induced by skin turnover) following skin exposure. In vitro skin permeation experiments revealed that the permeability coefficients of diazinon and resmethrin were smaller than the desquamation rate; therefore, these biocides could not permeate the skin, which resulted in very low skin concentrations of these compounds. On the other hand, the skin concentration of p-cresol was high because of its higher permeability coefficient than the desquamation rate. Furthermore, low in vitro cell viability was reported for skin exposed to p-cresol. These results in the present study indicate that the method described herein is useful for predicting the toxicities of chemicals following their topical exposure.

PubMed

Analysis of hair follicle penetration of lidocaine and fluorescein isothiocyanate-dextran 4 kDa using hair follicle-plugging method. Reviewed

Horita Daisuke, Yoshimoto Masato, Todo Hiroaki, Sugibayashi Kenji

Drug Dev Ind Pharm 40 345 - 351 2014.03

Language：English Publishing type：Research paper (scientific journal)

Analysis of hair follicle penetration of lidocaine and fluorescein isothiocyanate-dextran 4 kDa using hair follicle-plugging method.

Horita Daisuke, Yoshimoto Masato, Todo Hiroaki, Sugibayashi Kenji

Drug Dev Ind Pharm 40 ( 3 ) 345 - 351 2014.03

Language：English Publishing type：Research paper (scientific journal)

OBJECTIVE: Skin appendages including hair follicles (hfs) and the stratum corneum (sc) are beginning to be recognized as important permeation pathways for the skin permeation of drugs, but their detailed role is not yet clear. To investigate the contribution of hfs to drug permeation, we conducted skin permeation tests by controlling the hf contribution with a hf-plugging method. METHOD: Lidocaine (LC) and fluorescein isothiocyanate-dextran 4 kDa (FD-4) were selected as model drugs and pig ear skin was used as model skin. RESULTS: Skin permeabilities of ionized LC and FD-4 decreased with hf-plugging, whereas no change was observed for the skin permeation of unionized LC. A fairly good correlation was found for ionized LC and FD-4 between skin permeability and the number of hfs plugged. Permeation parameters of model drugs for both skin pathways were calculated utilizing Fick's second law of diffusion. Consequently, the sc pathway could highly contribute to the permeation of unionized LC, since unionized LC shows markedly high partition to the sc. In contrast, the hf pathway could contribute to the permeation of ionized LC and FD-4, since these had high distributions to the hf pathway in spite of its very small surface area relative to whole skin surface area. CONCLUSION: The hf pathway must be important for the skin permeation of ionized compounds and hydrophilic high molecular compounds. hf-plugging is also a useful method for assessing the skin permeability of compounds through the hf pathway.

DOI： 10.3109/03639045.2012.762653

PubMed

Analysis of the pretreatment effect of ethanol on the stratum corneum- and hair follicular-penetration of drugs using the hair follicle-plugging method.

Horita Daisuke, Todo Hiroaki, Sugibayashi Kenji

Chem Pharm Bull (Tokyo) 62 ( 6 ) 578 - 585 2014

Language：English Publishing type：Research paper (scientific journal)

The hair follicle-plugging method was used to analyze the effects of EtOH on skin permeation pathways. METHODS: In vitro permeation experiments were performed on 4 model drugs [isosorbide mononitrate (ISMN), ionized lidocaine (ionized LC), fluorescein (FL), and fluorescein isothiocyanate (FITC)-dextran 4 kDa (FD-4)] using excised pig ear skin. The skin permeations of ionized LC, FL, and FD-4 were decreased by hair follicle-plugging. Hair follicle-plugging prevented the skin permeation of FL and FD-4 in EtOH-pretreated skin, but did not prevent that of ISMN. On the other hand, the effect of hair follicle-plugging on the permeation of ionized LC was different among the pretreatment conditions. These results indicate that the EtOH pretreatment greatly affected the aqueous pathway in the stratum corneum and hair follicles.

PubMed

Usefulness of pressure-sensitive adhesives as a pretreatment material before application of topical drug formulations and a peeling tape for excess stratum corneum layers.

Kikuchi Keisuke, Todo Hiroaki, Sugibayashi Kenji

Chem Pharm Bull (Tokyo) 62 ( 6 ) 559 - 567 2014

Language：English Publishing type：Research paper (scientific journal)

Two unique pressure-sensitive adhesive (PSA) tapes (PSA-A, -B) with different adhesive properties of commercial PSAs were prepared and evaluated for their usefulness as a pretreatment material prior to the application of transdermal therapeutic systems or topical drug formulations and also as a peeling agent against excess layers of the stratum corneum. In the present study, in vitro permeation experiments were conducted using vertical type diffusion cells and excised hairless rat or porcine skin from which the stratum corneum had been stripped several times with PSAs. The results obtained revealed that PSA-A and -B had higher stripping or peeling effects than those of the marketed PSAs. Marked changes were observed in skin barrier function before and after stripping using PSAs, and the enhancement effect on the skin permeation of drugs achieved by stripping the stratum corneum was markedly different between the PSAs. PSA-A, in particular, markedly improved skin permeation and the skin concentration of topically applied chemical compounds because it removed many layers of the stratum corneum when skin was stripped only a few times. In contrast, when PSA-B was used to pretreat the skin surface, the extent of skin permeation and concentration of drugs was safely increased because only a few layers of the stratum corneum were removed, even with repeated stripping. The enhancement effect achieved by PSA-B was not as high as that by PSA-A. Thus, stripping with PSA-A can be used as a penetration enhancement tool, whereas PSA-B can be used as a peeling material against excess layers of the stratum corneum.

PubMed

[In vitro and in silico approaches to evaluate usefulness and safety of chemical compounds applied or exposed on skin].

Sugibayashi Kenji, Todo Hiroaki, Kadhum Wesam R

Yakugaku Zasshi 134 ( 1 ) 27 - 32 2014

Language：Japanese Publishing type：Research paper (scientific journal)

Recently, animal experiments become very difficult to be done in the research and development of cosmetics and cosmeceuticals due to animal welfare and 3Rs (replacement, reduction, refinement) concept. However, usefulness and safety of these preparations must be strictly guaranteed before human use. We thus proposed three sets of extrapolation methods to estimate in vivo profiles from in vitro and in silico approaches, to evaluate permeation profiles through real human skin from those through animal skin and cultured human skin model, and to estimate responses such as usefulness and safety of cosmetics and cosmeceuticals from their skin permeation and concentration profiles. Although we need more data and discussion, the present extrapolation methods must be very useful for estimation of cosmetics and cosmeceuticals without using animal experiments.

PubMed

Effect of beta-cyclodextrin derivatives on the diosgenin absorption in Caco-2 cell monolayer and rats.

Okawara Masaki, Tokudome Yoshihiro, Todo Hiroaki, Sugibayashi Kenji, Hashimoto Fumie

Biol Pharm Bull 37 ( 1 ) 54 - 59 2014

Language：English Publishing type：Research paper (scientific journal)

Orally administrated diosgenin, a steroidal saponin found in the roots of Dioscorea villosa, improves reduced skin thickness in ovariectomized mice, and plays an important role in the treatment of hyperlipidemia. Diosgenin has been noticed as an active element in cosmeceutical and dietary supplements. We have already elucidated that the absolute oral bioavailability of diosgenin is very low; however, a high skin distribution of diosgenin was also observed. The aim of the present study was to examine and compare the effects of beta-cyclodextrin (beta-CD) and 3 kinds of its derivatives such as hydroxypropyl beta-CD on the diosgenin permeability using a Caco-2 model and rat jejunal perfusion. These derivatives of beta-CD greatly improved the low solubility of diosgenin. No significant increase was observed in the lactate dehydrogenase leakage from Caco-2 cell, while a slight decrease was found on the transepithelial electrical resistance by diosgenin and beta-CD derivatives. However, beta-CD derivatives, especially hydroxyethyl beta-CD and hydroxypropyl beta-CD, markedly enhanced diosgenin permeability across the Caco-2 monolayer and rat jejunum. The bioavailability of diosgenin in the presence of beta-CD derivatives were about 4 to 11 fold higher than diosgenin suspension. The mechanisms of these enhancement effects may be due to improvements in solubility and tight junction opening.

PubMed

Enhancement of diosgenin distribution in the skin by cyclodextrin complexation following oral administration.

Okawara Masaki, Tokudome Yoshihiro, Todo Hiroaki, Sugibayashi Kenji, Hashimoto Fumie

Biol Pharm Bull 36 ( 1 ) 36 - 40 2013

Language：English Publishing type：Research paper (scientific journal)

Orally administrated diosgenin, a steroidal saponin found in several plants including Dioscorea villosa, recovers skin thickness reduced in ovariectomized mice, and plays an important role in the treatment of hyperlipidemia. Thus, diosgenin is an active element of cosmeceutical and dietary supplements. However, we have already elucidated that the skin distribution and absolute oral bioavailability of diosgenin is very low. The aim of this study is to evaluate the efficacy of diosgenin-cyclodextrin (CD) complexes in improving the skin concentration of diosgenin. The formation of the CD complex was indicated by powder X-ray diffraction (XRD), differential scanning calorimetry (DSC), and scanning electron microscope (SEM) studies. Oral administration of the diosgenin/beta-CD complex resulted in a significant enhancement in terms of the skin distribution of diosgenin, maximum plasma level (C(max)), area under the plasma concentration-time curve (AUC), and absolute oral bioavailability over those of the drug alone. These results suggest that the inclusion complex of diosgenin/beta-CD can be used to improve low skin content of diosgenin.

PubMed

Mathematical model to predict skin concentration after topical application of drugs.

Todo Hiroaki, Oshizaka Takeshi, Kadhum Wesam R, Sugibayashi Kenji

Pharmaceutics 5 ( 4 ) 634 - 651 2013

Language：English Publishing type：Research paper (scientific journal)

Skin permeation experiments have been broadly done since 1970s to 1980s as an evaluation method for transdermal drug delivery systems. In topically applied drug and cosmetic formulations, skin concentration of chemical compounds is more important than their skin permeations, because primary target site of the chemical compounds is skin surface or skin tissues. Furthermore, the direct pharmacological reaction of a metabolically stable drug that binds with specific receptors of known expression levels in an organ can be determined by Hill's equation. Nevertheless, little investigation was carried out on the test method of skin concentration after topically application of chemical compounds. Recently we investigated an estimating method of skin concentration of the chemical compounds from their skin permeation profiles. In the study, we took care of "3Rs" issues for animal experiments. We have proposed an equation which was capable to estimate animal skin concentration from permeation profile through the artificial membrane (silicone membrane) and animal skin. This new approach may allow the skin concentration of a drug to be predicted using Fick's second law of diffusion. The silicone membrane was found to be useful as an alternative membrane to animal skin for predicting skin concentration of chemical compounds, because an extremely excellent extrapolation to animal skin concentration was attained by calculation using the silicone membrane permeation data. In this chapter, we aimed to establish an accurate and convenient method for predicting the concentration profiles of drugs in the skin based on the skin permeation parameters of topically active drugs derived from steady-state skin permeation experiments.

DOI： 10.3390/pharmaceutics5040634

PubMed

Effect of direction (epidermis-to-dermis and dermis-to-epidermis) on the permeation of several chemical compounds through full-thickness skin and stripped skin.

Oshizaka Takeshi, Todo Hiroaki, Sugibayashi Kenji

Pharm Res 29 ( 9 ) 2477 - 2488 2012.09

Language：English Publishing type：Research paper (scientific journal)

PURPOSE: Compound permeation through stratum corneum-stripped skin is generally greater than that through full-thickness skin. In addition, epidermis-to-dermis permeation profile should be the same as dermis-to-epidermis permeation profile. However, stripped skin permeability of some compounds was lower than full-thickness skin permeability and different permeabilities were found for some compounds between the two directions of skin permeation. The reasons for these findings were investigated in this study. METHODS: Full-thickness or stripped hairless rat skin was set in a Franz-type diffusion cell, and a solution of compound was applied on the epidermis or dermis side to determine the in vitro skin permeability. RESULTS: Although the stripped skin permeability of pentyl paraben (PeP) with extremely high logK(o/w) was lower than full-thickness skin permeabilities, the addition of 3% ethanol resulted in the expected permeation order. Epidermis-to-dermis permeation of PeP through full-thickness skin was higher than dermis-to-epidermis permeation. Epidermis-to-dermis permeations of fluorescein isothiocyanate dextran (FD-4) and isosorbide 5-mononitrate with negative logK(o/w) were also higher than those in the opposite direction. CONCLUSIONS: Morphological observation of skin after FD-4 permeation suggested that a conically shaped trans-follicular permeation pathway model could be advocated to explain the difference between the epidermis-to-dermis permeation and that in the opposite direction.

DOI： 10.1007/s11095-012-0777-6

PubMed

Effects of soybean peptide and collagen peptide on collagen synthesis in normal human dermal fibroblasts.

Tokudome Yoshihiro, Nakamura Kyosuke, Kage Madoka, Todo Hiroaki, Sugibayashi Kenji, Hashimoto Fumie

Int J Food Sci Nutr 63 ( 6 ) 689 - 695 2012.09

Language：English Publishing type：Research paper (scientific journal)

The collagen present in the dermis of the skin is a fibrous protein that fills the gaps between cells and helps maintain tissue flexibility. Effectively increasing the collagen present in the skin is an important goal for cosmetic research. Recent research has shown that soybean peptide (SP) has anti-fatigue activity, antioxidant activity, and the ability to increase type I collagen, while collagen peptide (CP) has the ability to enhance corneal moisture content and viscoelasticity, as well as to increase levels of hyaluronic acid synthesizing enzymes in human skin. Little documented research, however, has been conducted on collagen formation in relation to these peptides. Therefore, this research applied SP and CP with molecular weights primarily around 500 and preparations containing both SP and CP to normal human dermal fibroblasts together with magnesium ascorbyl phosphate (VC-PMg), and used real-time PCR to determine the gene expression of type I collagen (COL1A1), which contributes to collagen synthesis, and Smad7, which contribute to collagen breakdown. In addition, enzyme linked immuno sorbent assay (ELISA) was used to measure collagen content in the media. COL1A1 gene expression at 24 h after sample addition showed higher tendency in all samples and increased with time at 4, 8 and 24 h after addition. Smad7 gene expression was not substantially different at 4 h after addition. matrix metalloproteinase-1 gene expression was higher following SP addition, but was lower after the addition of CP and SP+CP. Medium collagen content was higher in all samples and increased with time at 8 h after addition. Collagen levels were higher when SP and CP were added together.

DOI： 10.3109/09637486.2011.652597

PubMed

Measurement of skin permeation/penetration of nanoparticles for their safety evaluation. Reviewed

Biological & pharmaceutical bulletin 35 ( 9 ) 2012.04

Language：English Publishing type：Research paper (scientific journal)

Measurement of skin permeation/penetration of nanoparticles for their safety evaluation.

Kimura Eriko, Kawano Yuichiro, Todo Hiroaki, Ikarashi Yoshiaki, Sugibayashi Kenji

Biol Pharm Bull 35 ( 9 ) 1476 - 1486 2012

Language：English Publishing type：Research paper (scientific journal)

The aim of the present study was to quantitatively evaluate the skin permeation/penetration of nanomaterials and to consider their penetration pathway through skin. Firstly, penetration/permeation of a model fluorescent nanoparticle, Fluoresbrite(R), was determined through intact rat skin and several damaged skins. Fluoresbrite(R) permeated through only needle-punctured skin. The permeation profiles of soluble high molecular compounds, fluorescein isothiocyanate-dextrans (FITC-dextrans, FDs), with different molecular weights were also measured for comparison. The effects of molecular sizes and different skin pretreatments on the skin barrier were determined on the skin penetration/permeation of Fluoresbrite(R) and FDs. Fluoresbrite(R) was not permeated the intact skin, but FDs were permeated the skin. The skin distribution of titanium dioxide and zinc oxide nanoparticles was also observed after topical application of commercial cosmetics. Nanoparticles in sunscreen cosmetics were easily distributed into the groove and hair follicles after their topical application, but seldom migrated from the groove or follicles to viable epidermis and dermis. The obtained results suggested that nanoparticles did not permeate intact skin, but permeated pore-created skin. No or little permeation was observed for these nanomaterials through the stratum corneum.

PubMed

[Safety and skin penetration of nanoparticles].

Kimura Eriko, Todo Hiroaki, Sugibayashi Kenji

Yakugaku Zasshi 132 ( 3 ) 319 - 324 2012

Language：Japanese Publishing type：Research paper (scientific journal)

Human beings are exposed or otherwise a subjected to a various chemical compounds. Various nanomaterials are contained in the chemical compounds which are used in many fields. Nanomaterials are also used in cosmetics: titanium dioxide and zinc oxide are examples. Consumers who apply cosmetics to their skin as well as workers at industrial plants may thus be exposed to these nanoparticles. Therefore, it is of great importance to evaluate the safety of these nanoparticles. In this review, we describe the possibility of nanoparticle penetration to skin following exposure, which makes it urgent to evaluate the safety factors. In general, it is necessary to take account of the desquamation rate of the stratum corneum and the permeation pathway and size of nanoparticles when considering such penetration. One layer of the human stratum corneum is peeled off per day. Therefore, a chemical compound of which the skin penetration is lower than the desquamation rate does not permeate through the skin, when the compound infiltrates the stratum corneum. Hence, compounds with a molecular weight of more than 500 Daltons do not permeate through the stratum corneum. However, we must also pay attention to the appendage routes, although the aforementioned layer is the primary permeation route of nanoparticles. The contribution of appendage routes must be taken into consideration.

PubMed

Influence of skin thickness on the in vitro permeabilities of drugs through Sprague-Dawley rat or Yucatan micropig skin.

Takeuchi Hiroyuki, Ishida Masahiro, Furuya Atsushi, Todo Hiroaki, Urano Hidetoshi, Sugibayashi Kenji

Biol Pharm Bull 35 ( 2 ) 192 - 202 2012

Language：English Publishing type：Research paper (scientific journal)

The purpose of this study was to clarify the influence of skin thickness on the in vitro permeabilities of 3 model drugs with different physicochemical properties (nicorandil (NR), isosorbide dinitrate (ISDN) and flurbiprofen (FP)) through Sprague-Dawley rat (rat) or Yucatan micropig (YMP) skin. Intact, dermis-split, stratum corneum-stripped or stratum corneum-stripped and dermis-split rat or YMP skin (rat skin thickness: approximately 0.4, 0.9 or 1.2 mm; YMP skin thickness: approximately 0.4, 0.9, 1.8 or 2.8 mm) were set in Franz-type diffusion cells to determine the permeation rate, lag time and resistance ratio of the viable epidermis and dermis against whole skin (R(ved)/R(tot)) of the drugs. The YMP skin permeabilities of the drugs decreased with an increase in the skin thickness, and significant differences were observed in the permeation rates and lag times between intact and dermis-split (0.4 mm) YMP skins. The decreases in the permeabilities of the drugs through the YMP skin were larger than those through the rat skin. The influence of resistances of ISDN and FP through the dermis-split rat or YMP skin was greater at 0.9 mm skin thickness than 0.4 mm skin thickness. The R(ved)/R(tot) values for the YMP skins were relatively large for lipophilic drugs (ISDN and FP), and these ratios increased with an increase in the dermis thickness. These results suggest that in vitro skin permeation studies must be done using dermis-split (0.4 mm) skin with the thinnest dermis for predicting in vivo human percutaneous absorption rate.

PubMed

[Evaluation of efficacy and safety of drugs absorbed through skin using their physicochemical parameters].

Oshizaka Takeshi, Todo Hiroaki, Sugibayashi Kenji

Yakugaku Zasshi 132 ( 11 ) 1237 - 1243 2012

Language：Japanese Publishing type：Research paper (scientific journal)

Skin has been paid attention as a site of application of prescription drugs, over-the-counter drugs (non-prescription drugs) and cosmetics. Skin permeation and skin concentration of the compounds should be considered after topical administration, as well as their blood concentration to evaluate efficacy and safety. Since the evaluation of the amount of drugs permeated through skin is important for topically applied drugs, studies on the skin permeation has been greatly advanced. In addition, many reports proved that skin permeabilities of drugs could be predicted from physicochemical parameters of drugs. On the other hand, few reports have been found on the prediction of skin concentration of drugs. Furthermore, many experimented problems are left to determine the skin concentration of drugs: severe consume of human or animal skins, difficult removal of applied drugs from the skin surface, low drug extraction ratio from skin and low sensitivity to determine skin concentration of drugs, and requirement of long time measurement. Thus, fast and accurate measurement of skin concentration of applied drugs are urgently required. This report describes the relationship between skin permeation and skin concentration, and the prediction of skin concentration of drugs using skin permeation parameters of drugs.

PubMed

Evaluation of the skin blanching of topically applied steroids using a chroma meter in animals.

Ishii Hiroshi, Fujino Konomi, Todo Hiroaki, Sugibayashi Kenji

Exp Anim 61 ( 2 ) 147 - 156 2012

Language：English Publishing type：Research paper (scientific journal)

We evaluated the utility of animal skins for determining the skin blanching of steroids. A Chroma Meter was used to determine the skin blanching of steroids. Hydrophilic creams containing clobetasol propionate (CP) or prednisolone (PS) were selected as model steroid formulations. Skin blanching, a*, was determined using a Chroma Meter after the application of 0.005, 0.01, 0.1, or 1.0% CP or PS hydrophilic cream to the back skin of guinea pigs and hairless rats for 24 h. The relationships between Deltaa*(6h) and the skin concentrations of the steroids were determined at 6 h after removal of the cream. Deltaa*(6h) was markedly decreased after the application of CP hydrophilic cream to guinea pigs, and a good linear relationship was observed between Deltaa*(6h) and skin concentration (r=0.98). In contrast, no relationship was observed between these parameters after the application of CP cream to the hairless rats. Although skin blanching was observed after PS cream application in guinea pigs, no relationship was observed between Deltaa*(6h) and skin concentration of PS in each animal. These results suggest that the skin blanching effect of CP in guinea pigs is greater than that of PS and that its blanching effect in guinea pigs was stronger than that in hairless rats. Guinea pigs were found to be a good animal model for determining the skin blanching produced by steroid creams. In addition, Chroma Meters can be effectively used in skin vasoconstrictive tests in guinea pigs.

PubMed

Effect of ethanol pretreatment on skin permeation of drugs.

Horita Daisuke, Todo Hiroaki, Sugibayashi Kenji

Biol Pharm Bull 35 ( 8 ) 1343 - 1348 2012

Language：English Publishing type：Research paper (scientific journal)

It has been demonstrated that ethanol (EtOH) can enhance skin permeation of drugs when simultaneously applied with drugs. However, only a few studies have reported on the pretreatment effect of EtOH on skin permeations. In this study, the pretreatment effects of EtOH on skin permeation of drugs were investigated by measuring changes in skin permeation and electrical skin resistance. Permeabilities of deuterium oxide (D2O), isosorbide mononitrate (ISMN), isosorbide dinitrate (ISDN), calcein sodium (CA-Na), and fluorescein isothiocyanate-dextran 4 kDa (FD-4, 3.3-4.4 kDa) were evaluated through Yucatan micropig skin pretreated with different concentrations of EtOH solution. From the results, almost constant skin permeabilities of D2O and ISDN were observed independent of EtOH concentration. Skin permeabilities of ISMN, CA, and FD-4 increased with low concentrations of EtOH, but decreased with high concentrations of EtOH. At 99.5% EtOH pretreatment, skin permeabilities of hydrophilic compounds (ISMN, CA, and FD-4) decreased to non-detectable levels. In addition, low molecular ion transports were almost constant at any EtOH concentration. Since molecular (ion) sizes of ISMN, CA, and FD-4 are larger than Na+, Cl-, and D2O, permeation pathway sizes for hydrophilic compounds in the skin barrier may be remarkably decreased by pretreatment with high concentrations of EtOH. However, the permeability coefficient of ISDN was not influenced by any EtOH concentration, since ISDN is a lipophilic, low-molecular compound that permeated through the lipophilic stratum corneum pathway. The present results show useful information for repeatedly and topically applied formulations containing EtOH, and also contribute to the effective use of alcohol formulations.

PubMed

Pretreatment effects of moxibustion on the skin permeation and skin and muscle concentrations of salicylate in rats.

Cao Dianxiu, Tazawa Yuko, Ishii Hiroshi, Todo Hiroaki, Sugibayashi Kenji

Int J Pharm 407 ( 1-2 ) 105 - 110 2011.04

Language：English Publishing type：Research paper (scientific journal)

The effect of moxibustion on the in vitro and in vivo skin permeation of salicylate was evaluated in rats. First, the effect of moxibustion pretreatment on the elimination pharmacokinetics of salicylate after i.v. injection in rats was determined: no clear difference was observed in the plasma profiles of salicylate (SA) with or without moxibustion pretreatment. However, much higher skin and muscle concentrations of salicylate were observed after its i.v. injection. Next, an in vitro skin permeation study of SA was performed after moxibustion pretreatment. Moxibustion pretreatment increased the skin permeation of SA, and the extent of the increase in SA skin permeation was related to the strength of moxibustion ignition. More intense treatments produced higher skin permeation. A similar enhancement effect on the skin permeation of SA was observed in in vivo studies. Interestingly, the skin/plasma and muscle/plasma ratios of SA were markedly increased by moxibustion pretreatment. These results were due to the induction of enhanced skin permeation and lower clearance into the cutaneous vessels by moxibustion ignition. Combination treatment involving moxibustion and the topical application of drugs such as NSAID may be useful for increasing local pharmaceutical effects by enhancing the drug concentration in the skin and muscle underneath the topical application site.

DOI： 10.1016/j.ijpharm.2011.01.026

PubMed

Iontophoresis-facilitated delivery of prednisolone through throat skin to the trachea after topical application of its succinate salt.

Ishii Hiroshi, Suzuki Tsukasa, Todo Hiroaki, Kamimura Mitsuhiro, Sugibayashi Kenji

Pharm Res 28 ( 4 ) 839 - 847 2011.04

Language：English Publishing type：Research paper (scientific journal)

PURPOSE: The possibility of direct delivery of steroids through the skin to the trachea and the effect of iontophoresis on delivery efficacy were evaluated after the application of an ionic steroidal prodrug, prednisolone sodium succinate (PS-Na), to the throat skin. METHODS: Fluorescein sodium salt (FL-Na) and PS-Na were applied as model compounds at a concentration of 1% in pH 7.4 phosphate-buffered saline to the throat skin of hairless rats, and constant current-cathodal iontophoresis (0.4 mA/cm(2)) was performed for 8 or 10 h. RESULTS: In vitro permeation experiment involving cathodal iontophoresis through excised hairless rat abdominal skin revealed 30- and 10-times higher levels of skin permeation of PS and its active drug, prednisolone (P), than those induced without iontophoresis. In vivo iontophoresis treatment of the rat's throat skin produced 2.6-, 1.6- and 12-times higher FL, PS and P concentrations, respectively, in the trachea than those observed without iontophoresis. CONCLUSION: The present results suggest the usefulness of topical application of the ionic steroidal prodrugs onto throat skin followed by iontophoresis treatment for directly delivering the steroid to the trachea.

DOI： 10.1007/s11095-010-0337-x

PubMed

Preparation and evaluation of liquid-crystal formulations with skin-permeation-enhancing abilities for entrapped drugs.

Yamada Keisuke, Yamashita Jun, Todo Hiroaki, Miyamoto Keiko, Hashimoto Satoru, Tokudome Yoshihiro, Hashimoto Fumie, Sugibayashi Kenji

J Oleo Sci 60 ( 1 ) 31 - 40 2011

Language：English Publishing type：Research paper (scientific journal)

The usefulness of liquid crystals (LC) in topical formulations for application to skin was evaluated by measuring the in vitro permeation profile of a model compound, calcein, entrapped in a LC formulation, through excised hairless rat skin and a three-dimensional cultured human-skin model; the viability was determined using the MTT assay. Two physically stable LCs were prepared from a mixture of mono-, di-, and tri-esters 1, and monoesters 2, composed of erythritol and phytanylacetic acid. Cryo-transmission electron microscopy (cryo-TEM), electron diffraction patterns, and small-angle X-ray diffraction (SAXS) observations of the LC nanodispersions showed that the structures of the LCs were reverse hexagonal (LC-A) and cubic (LC-B). The skin-permeation properties of calcein were enhanced by entrapping in the LCs as a result of the increase in calcein partition from the LC dispersion solution into the skin; the properties were analyzed using a skin-permeation-time profile. Drug partitioning could also be modified by the LC structure. No skin damage was caused by the LC formulation in these experiments.The present study suggests that LC dispersions are potential additives in topical drug formulations and cosmetic formulations.

PubMed

Increase in ceramide level after application of various sizes of sphingomyelin liposomes to a cultured human skin model.

Tokudome Y, Jinno M, Todo H, Kon T, Sugibayashi K, Hashimoto F

Skin Pharmacol Physiol 24 ( 4 ) 218 - 223 2011

Language：English Publishing type：Research paper (scientific journal)

Sphingomyelin-based liposomes (SPM-L) that were sized (or not) by extrusion through a filter with pores of 100, 200, or 400 nm were applied to a three-dimensional cultured human skin model in order to evaluate which size of SPM-L was most effective at increasing its ceramide level. The diameters of the SPM-L in PBS were 102.7, 181.0, 224.0, and 380.1 nm. The diameters of the liposomes in the culture medium were 117.5, 199.2, 242.1, and 749.8 nm. The diameter of the small liposomes (<200 nm in diameter) did not change much, at least for 7 days. SPM-L in saline or culture medium were applied to the basal layer side or stratum corneum side of the cultured skin model, and ceramide II, III, V, and VI were then extracted from it. The extracted ceramide molecules were separated by HPTLC, and the concentration of each type of ceramide was quantified using a densitometer. When the small SPM-L (110 or 190 nm in diameter) were applied to the basal layer side, the levels of ceramide III and V were increased. When they were applied to the stratum corneum side, the levels of ceramide II, III, V, and VI were significantly increased compared to those of the PBS group, especially after the application of the small SPM-L (110 nm in diameter). Thus, the application of small SPM-L was useful for increasing the ceramide II, III, V, and VI levels of a cultured human skin model.

DOI： 10.1159/000324886

PubMed

Preparation and evaluation of liquid-crystal formulations with skin permeation-enhancing abilities for entrapped drug Reviewed

Keisuke Yamada, Jun Yamashita, Keiko Miyamoto, Satoru Hashimoto, Yoshihiro Tokudome, Fumie Hashimoto and Kenji Sugibayashi

Journal of Oleo Science 2010.12

Language：English Publishing type：Research paper (scientific journal)

Utilization of reconstructed cultured human skin models as an alternative skin for permeation studies of chemical compounds Reviewed

Kano S, Todo H, Sugie K, Fujimoto H, Nakada K, Tokudome Y, Hashimoto F, Sugibayashi K

AATEX 15 ( 2 ) 2010.12

Language：English Publishing type：Research paper (scientific journal)

Utilization of reconstructed cultured human skin models as an alternative skin for permeation studies of chemical compounds Reviewed

Kano S., Sugie K., Fujimoto H., Nakada K., Tokudome Y., Hashimoto F., Sugibayashi K.

AATEX 15 ( 2 ) 61 - 70 2010.12

Authorship：Second author Language：English Publishing type：Research paper (scientific journal)

Other Link： http://libir.josai.ac.jp/il/meta_pub/G0000284repository_JOS-13440411-15_2

Macromolecular delivery into skin using hollow microneedles Reviewed

Wonglertnirant N., Opanasopit P., Ngawhirunpat T., Sugibayashi K

Biological & Pharmaceutical Bulletin 2010.12

Authorship：Second author Language：English Publishing type：Research paper (scientific journal)

Other Link： http://libir.josai.ac.jp/il/meta_pub/G0000284repository_JOS-bpb.33.1988

Moisture enhancement of noisomes entrapped with mineral water in pig ear skin Reviewed

Manosroi A., Witkittilak W., Chutoprapat R., Sugibayashi K., Manosroi W., Manosroi J.

Chiang Mai J. Sci. 2010.10

Language：English Publishing type：Research paper (scientific journal)

Transdermal drug delivery by in-skin electroporation using a microneedle array Reviewed

Keshu Yan, Hiroaki Todo, Kenji Sugibayashi

International Journal of Pharmaceutics 397 ( 1-2 ) 77 - 83 2010.09

Authorship：Second author Language：English Publishing type：Research paper (scientific journal)

Other Link： http://libir.josai.ac.jp/il/meta_pub/G0000284repository_JOS-j.ijpharm.2010.06.052

Permeation pathway of macromolecules and nanospheres through skin Reviewed

Kimura E., Yasuno H., Tokudome Y., Hashimoto F., Ikarashi Y., Sugibayashi K.

Biol. Pharm. Bull. 2010.09

Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)

Other Link： http://libir.josai.ac.jp/il/meta_pub/G0000284repository_JOS-bpb.33.1394

Effect of negative charged particles on the recovery of skin barrier function after EP treatment Reviewed

K. Sugibayashi, H. Todo, K. Yamaguchi

Journal of Drug Delivery Science and Technology 20 ( 6 ) 2010.04

Language：English Publishing type：Research paper (scientific journal)

Macromolecular delivery into skin using a hollow microneedle Reviewed

Nanthida Wonglertnirant, Hiroaki Todo, Praneet Opanasopit, Tanasait Ngawhirunpat, Kenji Sugibayashi

Biological and Pharmaceutical Bulletin 33 ( 12 ) 2010.04

Language：English Publishing type：Research paper (scientific journal)

Effect of thermodynamic activity on skin permeation and skin concentration of triamcinolone acetonide Reviewed

Hiroshi Ishii, Hiroaki Todo, Kenji Sugibayashi

Chemical and Pharmaceutical Bulletin 58 ( 4 ) 2010.04

Language：English Publishing type：Research paper (scientific journal)

Effect of sebum and ointment rubbing on the skin permeation of triamcinolone acetonide from white petrolatum ointment Reviewed

Ishii H., Todo H., Sugibayashi K.

Biol. Pharm. Bull. 2010.04

Language：English Publishing type：Research paper (scientific journal)

Other Link： http://libir.josai.ac.jp/il/meta_pub/G0000284repository_JOS-bpb.33.876

Mathematical model to predict skin concentration of drugs: Toward utilization of silicone membrane to predict skin concentration of drugs as an animal testing alternative, Reviewed

Sugibayashi K., Todo H., Oshizaka T., Owada Y.

Pharm. Res. 2010.04

Authorship：Second author Language：English Publishing type：Research paper (scientific journal)

静脈内および経口投与後のディオスゲニンのラットにおける体内動態 Reviewed

大川原正喜、徳留嘉寛、藤堂浩明、杉林堅次、橋本フミ惠

薬剤学 2010.04

Language：Japanese Publishing type：Research paper (scientific journal)

Other Link： http://libir.josai.ac.jp/il/meta_pub/G0000284repository_JOS-03727629-70_82

Possibility and effectiveness of drug delivery to skin by needle-free injector Reviewed

Inoue N., Iidaka D., Tokudome Y., Hashimoto F., Kishino T., Sugibayashi K.

Int. J. Pharm. 391 2010.03

Authorship：Second author Language：English Publishing type：Research paper (scientific journal)

Other Link： http://libir.josai.ac.jp/il/meta_pub/G0000284repository_JOS-j.ijpharm.2010.02.019

Effect of topically applied sphingomyelin-based liposomes on the ceramide level in a three dimensional cultured human skin model Reviewed

Tokudome Y, Uchida, Yokote T., Todo H., Hada N., Kon T., Yasuda J., Hayashi H., Hashimoto F., Sugibayashi K.

J. Liposome Res 20 ( 1 ) 49 - 54 2010.03

Language：English Publishing type：Research paper (scientific journal)

Other Link： http://libir.josai.ac.jp/il/meta_pub/G0000284repository_JOS-08982100903062597

Skin permeation and transdermal delivery systems of drugs: History to overcome barrier function in the stratum corneum Reviewed

Masahiro Sugino, Hiroaki Todo, Kenji Sugibayashi

Yakugaku Zasshi 129 ( 12 ) 1453 - 1458 2009.12

Language：English Publishing type：Research paper (scientific journal)

Bactericidal Activities of Several Electrolyzed Waters Reviewed

43 ( 2 ) 101 - 106 2009.04

Language：Japanese Publishing type：Research paper (scientific journal)

Why does a hydrophilic drug permeate skin, although it is not soluble in white petrolatum? Reviewed

Ishii H., Todo H., Terao A., Hasegawa T., Akimoto M., Oshima K., Sugibayashi K.

Drug Dev. Ind. Pharm. 2009.04

Language：English Publishing type：Research paper (scientific journal)

Effect of molecular weight on the dermatopharmacokinetics and systemic disposition of drugs after intracutaneous injection Reviewed

Yoshida D., Todo H., Sugibayashi K.,

Europ. J. Pharm. Sci. 2008.04

Authorship：Second author Language：English Publishing type：Research paper (scientific journal)

電解水を用いた界面活性剤フリーエマルションの作製と皮膚透過性制御に関する研究, Reviewed

北村敏彦、小池真理子、藤堂浩明、岡島眞裕、石井文由、杉林堅次

香粧品学会誌 2008.04

Language：Japanese Publishing type：Research paper (scientific journal)

Analysis of skin disposition and metabolism of ethyl nicotinate after topical application using dual agar gel disc-inserted rats Reviewed

Hasegawa T., Kawazome A., Matsumoto K., Yanagimoto G., Hayashi T., Akimoto M., Todo H., Sugibayashi K.,

Biol. Pharm. Bull., 2008.04

Language：English Publishing type：Research paper (scientific journal)

Effect of vasoactive agents on the dermatopharmacokinetics and systemic disposition of model compounds, salicylate and FITC-dextran 4 kDa, following intracutaneous injection of the compounds

Yoshida D., Todo H., Hasegawa T., Sugibayashi K.

Int. J. Pharmaceut 2008.04

Language：English Publishing type：Research paper (scientific journal)

Safety evaluation of titanium dioxide nanoparticles by their absorption and elimination profiles

Sugibayashi K., Todo H., Kimura E.

J. Toxicol. Sci. 2008.04

Language：English Publishing type：Research paper (scientific journal)

Effect of several electrolyzed waters on the skin permeation of lidocaine, benzoic acid and isosorbide mononitrate Reviewed

Kitamura T., Todo H., Sugibayashi K.,

Drug Develop. Ind. Pharm. 2008.04

Language：English Publishing type：Research paper (scientific journal)

Combined effect of sodium chondroitin sulfate and sodium hyaluronate on skin moisuturization following single and repeated application, Reviewed

Ishii H., Kitamura T., Todo H., Sugibayashi K., Sakurai H., Fukahori M.

Asian J. Pharm. Sci. 2008.04

Language：English Publishing type：Research paper (scientific journal)

Analysis of skin disposition of flurbiprofen after topical application using dual agar gel discs-inserted rats Reviewed

Tetsuya Hasegawa, Asako Kawazome, Go Yanagimoto, Teruaki Hayashi, Toshinobu Seki, Masayuki Akimoto, Hiroaki Todo, Kenji Sugibayashi

Biological and Pharmaceutical Bulletin 30 ( 11 ) 2135 - 2140 2007.11

Language：English Publishing type：Research paper (scientific journal)

Effects of surface processing of lactose carrier particles on dry powder inhalation properties of salbutamol sulfate Reviewed

Kotaro Iida, Yukari Inagaki, Hiroaki Todo, Hirokazu Okamoto, Kazumi Danjo, Hans Luenberger

Chemical and Pharmaceutical Bulletin 52 ( 8 ) 2007.08

Language：English Publishing type：Research paper (scientific journal)

Dermatopharmacokinetics of salicylate following topical injection in rats: Effect of osmotic pressure and injection volume on salicylate disposition Reviewed

Daisuke Yoshida, Hiroaki Todo, Tetsuya Hasegawa, Kenji Sugibayashi

International Journal of Pharmaceutics 337 142 - 147 2007.06

Language：English Publishing type：Research paper (scientific journal)

Pharmacokinetic profile of flavin adenine dinucleotide, flavin mononucleotide and riboflavin following intravenous administration of riboflavin or its coenzymes in rats

Masayuki Akimoto, Yuko Sato, Eiichi Nemoto, Hiroaki Todo, Tetsuya Hasegawa, Kenji Sugibayashi

Journal of Health Science 53 ( 3 ) 2007.06

Language：English

Survey on safety and usefulness of Teicoplanin in the treatment of methicillin-resistance Staphylococcus Aureus (MRSA) infection Reviewed

Dai Iidaka, Hiroshi Ishii, Hiroaki Todo, Kenji Sugibayashi, Hiroshi Matsumoto, Yuko Sakashita, Mieko Yokoyama, Chuji Yanagawa

Japanese Pharmacology and Therapeutics 35 ( 2 ) 121 - 127 2007.04

Language：Japanese Publishing type：Research paper (scientific journal)

Enhancement of skin permeation of high molecular compounds by a combination of microneedle pretreatment and iontophoresis Reviewed

Xue Ming Wu, Hiroaki Todo, Kenji Sugibayashi

Journal of Controlled Release 118 ( 2 ) 2007.04

Language：English Publishing type：Research paper (scientific journal)

Preparation and Evaluation of Gene-transfected Cultured Skin as a Novel Drug Delivery System for Severely Burned Skin

Hada N,Todo H,Komada F

Pharm Res 2007.01

Language：English Publishing type：Research paper (scientific journal)

MRSA感染症治療におけるテイコプラニンの安全性・有効性調査

飯高大，松本浩，坂下裕子，柳川忠二，横山美恵子，石井宏，藤堂浩明，杉林堅次

薬理と治療 2007.01

Language：Japanese Publishing type：Research paper (scientific journal)

Enhancement of skin permeation of high molecular compounds by a combination of microneedle pretreatment and iontophoresis

Wu XM, Todo H,Sugibayashi K

J Control Release 2007.01

Language：English Publishing type：Research paper (scientific journal)

Dermatopharmacokinetics of salicylate following topical injection in rats:Effect of osmotic pressure and injection volume on salicylate disposition

Yoshida D, Todo H, Hasegawa T, Sugibayashi K

Int J Pharm 2007.01

Language：English Publishing type：Research paper (scientific journal)

Conversion of FDA to FMN nad riboflavin in plasma: Effects of measuring method

Akimoto.M , Sato.Y , Okubo.T , Todo.H , Hasegawa.T , Sugibayashi.K

Ciol Pharm Bull 2006.01

Language：English Publishing type：Research paper (scientific journal)

皮膚保湿性に及ぼすコンドロイチン硫酸ナトリウムとヒアルロン酸ナトリウムの併用効果

石井宏，北村敏彦，藤堂浩明，櫻井英知，深堀勝博，杉林堅次

薬理と治療 2006.01

Language：Japanese Publishing type：Research paper (scientific journal)

病態変動に伴う薬物の血漿中濃度変動パターンを推定する簡易ソフトの開発

石井宏，藤堂浩明，長谷川哲也，杉林堅次，緒方宏泰

医療薬学 32 ( 5 ) 445 - 454 2006.01

Language：Chinese Publishing type：Research paper (scientific journal)

Other Link： http://libir.josai.ac.jp/il/meta_pub/G0000284repository_JOS-1346342X32_445

Effects of pretreatment of needle puncture and sandpaper abrasion on the in vitro skin permeation of fluorecein isothiocyante (FITC)-dextran

WuX-M,Todo H,Sugibayashi K

Int J Pharm 2006.01

Language：English Publishing type：Research paper (scientific journal)

Preparation of dry powder inhalation with lactose carrier particles surface-coated using a Wurster Fluidized bed

Iida K,Todo H,Okamoto H,Danjo K,Keuenberger H

Chem Pharm Bull(Tokyo) 431 - 444 2005.01

Language：English Publishing type：Research paper (scientific journal)

Stability of chitosan-pDNA complex powder prepared by supercritical carbon dioxide process

Okamoto H,Sakakura Y,Shiraki K,Oka K,Nishida S,Todo H,Iida K,Danjo,K

Int J Pharm 73 - 81 2005.01

Language：English Publishing type：Research paper (scientific journal)

Effects ofsurface processing of lactose carrier particales on dry power inhalation properties of salbutamol sulfate

Iida K,Inagaki Y, Todo H,Okamoto H,Danjo K,Luenberger H

Chem.Pharm.Bull.(Tokyo) 938 - 924 2004.01

Language：English Publishing type：Research paper (scientific journal)

Improvement of stability and absorbability of dry insulin power for inhalation by powder-combination technique

Todo H,Okamoto H,Iida K,Danjo K

Int J Pharm 41 - 52 2004.01

Language：English Publishing type：Research paper (scientific journal)

Pulmonary gene delivery by chitosan-pDNA complex powder prepared by a supercritical carbon dioxide process

Okamoto H, Nishida S,Toda H,Sakakura Y,Iida K,Danjo K

J Pharm Sci 2003.01

Language：English Publishing type：Research paper (scientific journal)

Improvement of insulin absorption from intratracheally administrated dry power prepared by supercritical carbon dioxide process

Todo H,Okamoto H,Iida K,Danjo K

J Pharm Sci 2475 - 2486 2003.01

Language：English Publishing type：Research paper (scientific journal)

Dry powders for pulmonary delivery of peptides and proteins Reviewed

Hirokazu Okamoto, Hiroaki Todo, Kotaro Iida, Kazumi Danjo

KONA Powder and Particle Journal ( 20 ) 71 - 83 2002.04

Language：English Publishing type：Research paper (scientific journal)

Effect of additives on insulin absorption from intratracheally administered dry powders in rats Reviewed International journal

International Journal of Pharmaceutics 220 ( 1-2 ) 101 - 110 2001.01

Language：English Publishing type：Research paper (scientific journal)

　 PREV - NEXT 　

<TODO Hiroaki>

Papers - TODO Hiroaki