Papers - Komatsu Syuuhei
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Controlled phagocytosis of thermoresponsive core-corona type nanoparticles Reviewed
Akihiko Kikuchi, Masatoshi Kawase, Syuuhei Komatsu, Taka-Aki Asoh
Tissue Engineering and Regenerative Medicine International Society-AP Chapter and the 7th Asian Biomaterials Congress 2019.10
Language：English
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Drug Containing Carbonate Apatite Capsule Made from Biodegradable Coacervate Droplets for Bone Tissue Engineering Reviewed
Syuuhei KOMATSU, Taka-Aki ASOH, Akihiko KIKUCHI
Tissue Engineering and Regenerative Medicine International Society-AP Chapter and the 7th Asian Biomaterials Congress 2019.10
Authorship：Lead author Language：English
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Fabrication of thermoresponsive degradable hydrogel made by radical polymerization of 2-methylene-1,3-dioxepane: Unique thermal coacervation in hydrogel Reviewed International journal
Syuuhei Komatsu, Taka-Aki Asoh, Ryo Ishihara, Akihiko Kikuchi
POLYMER 179 2019.09
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
Thermoresponsive degradable hydrogels have attracted attention because their properties allow for their use as smart drug carriers for drug delivery systems and cell scaffolds in the living body. In this study, we synthesized thermoresponsive degradable hydrogels via radical copolymerization of 2-methylene-1,3-dioxepane and 2-hydroxyethylacrylate in the presence of a crosslinker at various feed ratios. The synthesized hydrogels showed a temperature-dependent swelling/shrinking behavior, which was also observed in the corresponding linear polymers. During shrinking, coacervate droplets were observed within the hydrogel. Under mild alkaline conditions, the hydrogels were degraded and converted into hydrophilic oligomers. Moreover, cell adhesion to the hydrogel surfaces was observed using poly (D-lysine)-modified hydrogels. The number of adhered cells increased on the hydrogels that showed shrinking behavior at 37 °C. The prepared hydrogels are expected to be applicable for use in functional cell scaffolding instead of as non-degradation materials.
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Design of a surface-functionalized power-free microchip for extracellular vesicle detection utilizing UV grafting Reviewed International journal
R. Ishihara, A. Katagiri, T. Nakajima, R. Matsui, S. Komatsu, K. Hosokawa, M. Maeda, Y. Tomooka, A. Kikuchi
REACT FUNCT POLYM 142 183 - 188 2019.07
Language：English Publishing type：Research paper (scientific journal)
As a biomarker for various diseases, extracellular vesicles (EV) in bodily fluids have received attention. In this study, poly(2-aminoethyl methacrylate hydrochloride) (PAEMA) was grafted onto a poly(dimethylsiloxane) power-free microchip by UV light-induced graft polymerization and anti-CD 63 antibody for EV capture was immobilized. Thus, a surface-functionalized power-free microchip (SF-PF microchip), which enables simple and rapid EV detection was fabricated. There was a correlation between the amount of antibody immobilized and the EV detection signal intensity, and both the amount and the signal intensity showed a maximum at 0.5 mol/L of AEMA concentration in feed for grafting. The sample volume required for EV detection was 1.0 μL and the time required for detection was 13 min. The SF-PF microchip would contribute to a point-of-care testing application.
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Preparation of thermo‐and redox‐responsive branched polymers composed of three‐armed oligo (ethylene glycol) Reviewed International journal
Syuuhei Komatsu, Hidenari Kayano, Yu Ando, Taka‐Aki Asoh, Ryo Ishihara, Akihiko Kikuchi
J POLYM SCI POL CHEM 56 ( 23 ) 2623 - 2629 2018.12
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
We herein report the preparation of thermo- and redox-responsive branched polymers by the condensation reaction of three-armed oligo(ethylene glycol) (trisOEG) and cystamine (CA). The prepared branched polymers exhibited a soluble–insoluble transition at a lower critical solution temperature (LCST) and formed coacervate droplets through a liquid–liquid phase separation process. We then demonstrated control of the LCSTs of the branched polymers by varying the feed ratio of CA and the surrounding salt concentration close to body temperature. In addition, the trisOEG-cysx polymer formed coacervate droplets above the LCST, in which hydrophobic molecules were condensed. The redox response of the branched polymers was also investigated. Interestingly, the branched polymers degraded to low-molecular-weight materials (i.e., trisOEG) in the presence of dithiothereitol as a reducing agent through cleavage of the disulfide bond of CA. This facile preparation of branched polymers is expected to be valuable in the context of functional biomedical materials and modifiers for materials surfaces, such as the bases for drug delivery carriers and separation materials. © 2018 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2018, 56, 2623–2629.
DOI： 10.1002/pola.29243
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Controlled Phagosytosis Of Relatively Hydrophilic Core-corona Type Spherical- Or Rod-shaped Particles Reviewed
Akihiko KIKUCHI Yota KOSUKEGAWA Masatoshi KAWASE Syuuhei KOMATSU Ryo ISHIHARA Taka-Aki ASOH
2018 Controlled Release Society Annual Meeting & Exposition 2018.06
Language：English
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Organic-inorganic Hybrid Core-shell Particles Made from Biodegradable Coacervate Droplets for Bone Tissue Engineering Reviewed
Syuuhei KOMATSU , Yui Ikedo, Taka Aki ASOH, Ryo ISHIHARA, Akihiko KIKUCHI
2018 Controlled Release Society Annual Meeting & Exposition 2018.06
Authorship：Lead author Language：English
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Fabrication of Hybrid Capsules via CaCO3 Crystallization on Degradable Coacervate Droplets Reviewed International journal
Syuuhei Komatsu, Yui Ikedo, Taka-Aki Asoh, Ryo Ishihara, Akihiko Kikuchi
Langmuir 34 ( 13 ) 3981 - 3986 2018.04
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal) Publisher：American Chemical Society
Organic-inorganic CaCO3 capsules were prepared by crystallization of CaCO3 on Pickering emulsion prepared using coacervate droplets made from thermoresponsive and degradable poly(2-methylene-1,3-dioxepane-co-2-hydroxyethyl acrylate) (poly(MDO-co-HEA)) in sole aqueous medium. The diameters of CaCO3-based Pickering emulsion could be controlled by varying several parameters: diameter of CaCO3 powders, initial polymer concentration, and copolymer composition. The CaCO3 Pickering emulsion was able to load low-molecular-weight hydrophobic substances at temperatures above the lower critical solution temperature (LCST) due to formation of polymer-concentrated phases, i.e., coacervate droplets. The diameter of CaCO3 capsules prepared by crystallization also depended on the diameter of the CaCO3 Pickering emulsion. The CaCO3 shell was composed of calcite-type crystals, the most stable polymorph among known CaCO3 crystals. The facially prepared CaCO3 capsules are valuable for use in functional biomaterials, such as drug delivery carriers and cell culture scaffolds for noninvasive bone-regenerative medicine.
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Facile preparation of degradable thermoresponsive polymers as biomaterials: Thermoresponsive polymers prepared by radical polymerization degrade to water-soluble oligomers Reviewed International journal
Syuuhei Komatsu, Taka-Aki Asoh, Ryo Ishihara, Akihiko Kikuchi
POLYMER 130 68 - 73 2017.11
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal) Publisher：ELSEVIER SCI LTD
A novel thermoresponsive and biodegradable polymer, poly(MDO-co-HEA), was prepared by radical copolymerization of 2-methylene-1,3-dioxepane (MDO) and a hydrophilic vinyl monomer, 2-hydroxyethyl acrylate (HEA), in dimethylsulfoxide (DMSO), varying feed monomer compositions. Poly( MDO-co-HEA) showed lower critical solution temperature (LCST)-type phase separation in aqueous medium, forming coacervate droplets into which low molecular weight hydrophobic molecules could be loaded above the LCST. The LCST could be controlled not only by the chemical compositions of the hydrophobic MDO and hydrophilic HEA in the polymer chains, but also by ion and polymer concentration. Degradation tests in aqueous media indicated that poly(MDO-co-HEA) was converted into hydrophilic oligomers by hydrolysis of the ester groups in the polymer backbone. The facile preparation poly(MDO-co-HEA) are valuable for use in functional biomedical materials, such as base of drug delivery carrier and cell culture scaffold instead of non-degradable stimuli-responsive polymer. (C) 2017 Elsevier Ltd. All rights reserved.