論文 - 小松 周平
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Preparation of thermoresponsive core-corona particles for controlled phagocytosis via surface properties and particle shape transformation 招待あり 査読あり 国際誌
Syuuhei Komatsu, Takuma Suzuki, Yota Kosukegawa, Masatoshi Kawase, Takuya Matsuyama, Taka-Aki Asoh, Akihiko Kikuchi
Journal of Controlled Release 381 113652 - 113652 2025年03月
担当区分：筆頭著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Elsevier BV
Cell-particle interactions, such as phagocytosis, exhibit variability based on particle shape, surface physical properties, and diameter. These interactions can be intentionally modified through in situ change in the physical characteristics of the particulate materials. By manipulating both the surface properties and shape of the particles, it may be feasible to regulate their interactions with cells. Objective of this research is to prepare thermoresponsive core-corona particles those undergo transformation and alteration in surface solubility near physiological temperature and to investigate particle shape- and surface physical property-dependent phagocytosis. The glass transition temperature of the prepared particles was controlled via the composition of the polymer core. Rod-type particles, prepared by uniaxially stretching particle-containing films at above the glass transition temperature of the core-forming materials, demonstrated reduced phagocytosis by macrophages compared to that of spherical particles. Furthermore, the physical properties of the particle surface exerted a significant influence on phagocytosis, with hydrophobic particles being more readily engulfed. Consequently, precise control of phagocytosis can be controlled by manipulating the particle's shape and surface properties. The prepared particles have potential applications as drug delivery system carriers, enabling the regulation of cell interactions via particle shape and surface physical properties induced by temperature changes.
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Thermoresponsive degradable hydrogels with renewable surfaces for protein removal 査読あり 国際誌
Syuuhei Komatsu, Naoki Kamei, Akihiko Kikuchi
Biomaterials Science 13 ( 1 ) 324 - 329 2025年01月
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In silico model to predict dermal absorption of chemicals in finite dose conditions. 査読あり
Ibuki Narita, Hiroaki Todo, Chihiro Fujiwara, Hiroyuki Teramae, Takeshi Oshizaka, Shoko Itakura, Syuuhei Komatsu, Kozo Takayama, Kenji Sugibayashi
The Journal of toxicological sciences 50 ( 4 ) 171 - 186 2025年
記述言語：英語 掲載種別：研究論文（学術雑誌）
The development of in silico approaches that can estimate the dermal absorption of chemicals exposed in practical conditions is highly anticipated. In the present study, an in silico model to estimate both the dermal absorption rate and dermal permeation profile was developed for the application of chemicals in finite dose conditions. Forty-three chemicals with molecular weights in the range 116-362 and logKo/w in the range 1.1-4.5 were used to develop an in silico model. A gradient boosting tree approach was applied to estimate permeation parameters for diffusion and partition coefficients of the chemicals in skin using physicochemical parameters of the chemicals such as molecular weight, lipophilicity, and the highest and lowest occupied molecular orbitals as the descriptor. In addition, 11 chemicals with different molecular weights and lipophilicities were applied on excised human skin in a finite dose condition, and dermal absorption profiles were obtained. Consideration of donor-solvent evaporation time, saturated concentrations of the chemicals, and donor-solvent coverage area on the skin surface, in addition to estimated skin permeation parameters of the chemicals, showed comparatively good dermal absorption profiles, although some cases of underestimation of dermal absorption were identified. It will be necessary to verify the accuracy of this model through experiments using more chemicals. However, the obtained results suggested that the established model may be valid to estimate the dermal absorption of chemicals in practical conditions.
DOI： 10.2131/jts.50.171
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Effect of the barrier function of stratum corneum and viable epidermis and dermis on the skin concentration of topically applied chemicals. 査読あり
Hiroaki Todo, Takeshi Oshizaka, Syuuhei Komatsu, Kenji Sugibayashi
The Journal of toxicological sciences 50 ( 4 ) 187 - 198 2025年
記述言語：英語 掲載種別：研究論文（学術雑誌）
Three-dimensional cultured skin (3D skin) models have been utilized for in vitro skin permeation tests to evaluate the skin permeation rate and local effects (efficacy and toxicity) of applied chemicals, particularly from the perspective of the 3Rs (reduction, replacement, refinement) approach. The steady-state concentration of applied chemicals at different depths in the viable epidermis and dermis (VED) is affected by their skin permeation parameters, such as permeability coefficient (Kp) and partition coefficient (K) from the donor solution to the skin of the chemicals. In the present study, the steady-state concentration of chemicals in the VED of EpiDerm 606X (EpiDerm) as representative of a 3D skin model were compared with hairless rat skin. The VED concentrations of chemicals in EpiDerm were higher than those in hairless rat skin when a model hydrophilic compound, antipyrine, and a model lipophilic compound, flurbiprofen, were applied, suggesting that the barrier functions of the VED against the whole skin were higher in EpiDerm than in hairless rat skin. When an ester compound, ethyl nicotinate, was applied, the VED concentration of nicotinic acid, a metabolite of ethyl nicotinate, was lower in EpiDerm than in hairless rat skin. These differences in the VED concentrations of applied chemicals might be related to false-positives and -negatives of topical effects evaluated with 3D skin models. It is important to pay particular attention to differences in VED concentration in 3D skin models and real skin when evaluating local efficacy and toxicity using 3D skin models.
DOI： 10.2131/jts.50.187
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Plasmid DNA Delivery into the Skin via Electroporation with a Depot-Type Electrode 査読あり 国際誌
Yuya Yoshida, Manami Aoki, Kalin Nagase, Koichi Marubashi, Hiroyuki Kojima, Shoko Itakura, Syuuhei Komatsu, Kenji Sugibayashi, Hiroaki Todo
Pharmaceutics 16 ( 9 ) 1219 - 1219 2024年09月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：MDPI AG
Objectives: Non-viral mediated plasmid DNA transfection by electroporation (EP) is an established method for gene transfection. In this study, the usefulness of direct EP at an intradermal (i.d.) site (DEP) with implanted electrodes to achieve a high protein expression level was investigated. In addition, DEP application with various intervals with a low application voltage was also evaluated to confirm its effect on protein expression. Methods: Green fluorescent protein (GFP)- and luciferase-encoding DNA were administrated, and GFP and luciferase were evaluated. Results: A higher protein expression level was observed after green fluorescent protein (GFP)- and luciferase-encoding DNA were delivered by i.d. injection followed by DEP application. When luciferase expression was observed with an in vivo imaging system, continuous expression was confirmed over 21 days after i.d. injection followed by DEP at 100 V. This approach provided increased gene expression levels compared with conventional EP methods via the stratum corneum layer. In addition, the effect of application voltage on luciferase expression was investigated; two-time applications (repeated DEP) at 20 V with 5 min intervals showed similar luciferase expression level to single DEP application with 100 V. Histological observations showed the skin became thicker after a single DEP at 100 V, whereas no apparent thickness changes were confirmed after repeated DEP at 20 V with 5 min intervals. Conclusions: This study revealed that direct i.d. voltage application achieved high protein expression levels even at low voltages. Skin is a promising administration site for DNA vaccines, so this approach may be effective for DNA vaccine delivery into skin tissue.
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Formation mechanism of anisotropic gelatin hydrogel by self-assembly on oriented templates 招待あり 査読あり
Kohei Kawaguchi, Tamaki Maeda, Syuuhei Komatsu, Yoshihiro Nomura, Kazuki Murai
Molecular Systems Design & Engineering 9 ( 6 ) 561 - 570 2024年06月
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Syuuhei Komatsu, Satoshi Yamada, Akihiko Kikuchi
ACS Biomaterials Science & Engineering 10 ( 2 ) 897 - 904 2024年01月
担当区分：筆頭著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：American Chemical Society (ACS)
Particle-cell interactions, such as cellular uptake, vary depending on the particle size, shape, and surface properties. By dynamic control of the physical properties of particles, microparticle-cell interactions can intentionally be altered. Particle degradability is also necessary for their application in the body. In this study, we aimed to prepare degradable core-corona-type particles that are deformed near the body temperature and investigated particle shape-dependent cellular uptake. Degradable and transformable particles consisting of poly(2-methylene-1,3-dioxepane)-co-poly(ethylene glycol) with three-armed poly(ε-caprolactone) (PCL) were prepared. The particle melting point was controlled by the chain length of the three-armed PCL. Particle degradation occurred under both acidic and alkaline conditions via ester group hydrolysis in the polymer backbones. The rod-shaped microparticles prepared by uniaxial stretching at a temperature above the melting point of the core showed less uptake into macrophages than did the spherical microparticles. Therefore, the degradable transformable particles enable macrophage interaction control via stimuli-regulated particle shapes and are expected to be applied as drug delivery carriers that can be decomposed and excreted from the body.
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温度刺激に応答する高分子微粒子の設計 招待あり 査読あり
小松 周平, 菊池 明彦
Journal of the Japan Society of Colour Material 96 ( 10 ) 338 - 343 2023年10月
担当区分：筆頭著者 記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Japan Society of Colour Material
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Yoshitsugu Akiyama, Kazunori Kimura, Syuuhei Komatsu, Tohru Takarada, Mizuo Maeda, Akihiko Kikuchi
ChemBioChem 24 ( 1 ) e202200451 2022年09月
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Degradation and drug release profile of degradable core-corona type particles under acidic condition for cancer treatment 査読あり 国際誌
Syuuhei Komatsu, Satomi Ishida, Taka-Aki Asoh, Akihiko Kikuchi
Reactive and Functional Polymers 177 105321 - 105321 2022年08月
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Preparation of carbonate apatite capsules based on degradable polymer-based coacervates and their osteoinductive effect on osteoblasts 査読あり
Syuuhei Komatsu, Taka-Aki Asoh, Akihiko Kikuchi
IUMRS-ICYRAM2022 2022年08月
担当区分：筆頭著者 記述言語：英語
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Facile fabrication of gelatin hydrogels with anisotropic gel properties via self-assembly 査読あり 国際誌
Kohei Kawaguchi, Syuuhei Komatsu, Akihiko Kikuchi, Yoshihiro Nomura, Kazuki Murai
Polymer Journal 54 ( 3 ) 377 - 383 2022年03月
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Protein Removal from Hydrogels through Repetitive Surface Degradation 査読あり 国際誌
Tatsuki Kamiya, Syuuhei Komatsu, Akihiko Kikuchi
ACS Applied Bio Materials 4 ( 12 ) 8498 - 8502 2021年12月
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Material Design of Drug Loadable Scaffold for Bone Regeneration and Treatment 招待あり 査読あり
Syuuhei Komatsu, Akihiko Kikuchi
Journal of the Society of Inorganic Materials, Japan 28 290 - 294 2021年09月
担当区分：筆頭著者 記述言語：日本語 掲載種別：論文集(書籍)内論文
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Facile preparation of multi-stimuli-responsive degradable hydrogels for protein loading and release. 査読あり 国際誌
Syuuhei Komatsu, Moeno Tago, Yu Ando, Taka-Aki Asoh, Akihiko Kikuchi
Journal of controlled release : official journal of the Controlled Release Society 331 1 - 6 2021年03月
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Facile preparation of 2-methylene-1, 3-dioxepane-based thermoresponsive polymers and hydrogels 査読あり 国際誌
Syuuhei Komatsu, Takuya Sato, Akihiko Kikuchi
POLYMER JOURNAL 53 ( 6 ) 731 - 739 2021年02月
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Development of carbonate apatite capsules based on degradable coacervate droplets for bone defect treatment 招待あり 査読あり
Syuuhei Komatsu
Drug Delivery System 36 ( 3 ) 216 - 217 2021年
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Organic-inorganic Hybrid Drug loadable Carbonate Apatite Capsules for Bone Tissue Engineering 査読あり
Syuuhei Komatsu, Shuhei Abe, Taka-Aki Asoh, Akihiko Kikuchi
11th World Biomaterial Congress 2020年12月
担当区分：筆頭著者 記述言語：英語
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骨欠損再生のための足場材料の展開 査読あり
小松周平、安部秀平、菊池明彦
BIOClinica 35 ( 7 ) 68 - 72 2020年06月
担当区分：筆頭著者 記述言語：日本語
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Preparation of thermoresponsive nanoparticles exhibiting biomolecule recognition ability via atom transfer radical dispersion polymerization. 査読あり 国際誌
Takuya Matsuyama, Hiroyuki Hayashi, Syuuhei Komatsu, Taka-Aki Asoh, Akihiko Kikuchi
Colloids and surfaces. B, Biointerfaces 183 110370 - 110370 2019年11月
記述言語：英語 掲載種別：研究論文（学術雑誌）
Thermoresponsive core-corona type nanoparticles were prepared exhibiting biomolecule recognition ability on their surfaces. These thermoresponsive nanoparticles were prepared from a poly(N-isopropylacrylamide) (PNIPAAm) macro-initiator and styrene (St) in a polar solvent via atom transfer radical dispersion polymerization. The PNIPAAm macro-initiator contains an alkyl halide and/or phthalimide group on the terminated group of the polymer chain, and thus, the grafting of PNIPAAm on the PSt core resulted in terminating phthalimide end groups. These terminal groups were utilized to immobilize biomolecule recognition units, and the dispersion stabilities of the nanoparticles were found to change in aqueous solution at room temperature due to alteration of the terminating PNIPAAm groups by the presence of biomolecules at different concentrations.