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Faculty of Pharmaceutical Sciences Department of Pharmaceutical Technochemistry |
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Komatsu Syuuhei
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Research Interests 【 display / non-display 】
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Hydrogel
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Biomaterial, Polymer chemistry
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Stimuli responsive polymer
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Organic-inorganic hydrid materials
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Biodegradable polymer
Research Areas 【 display / non-display 】
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Nanotechnology/Materials / Polymer materials / Polymeric Materials
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Life Science / Biomaterials / Biomaterials
From Graduate School 【 display / non-display 】
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Tokyo University of Science Graduate School, Division of Engineering Science Materials Engineering Doctor's Course Completed
- 2019
Country：Japan
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Tokyo University of Science Faculty of Engineering Science Materials Engineering Doctor's Course Completed
- 2014
Country：Japan
External Career 【 display / non-display 】
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Josai University Faculty of Pharmaceutical Sciences Department of Pharmaceutical Assistant Professor
2024.04
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Tokyo University of Science Faculty of Advanced Engineering Department of Materials Science and Technology Assistant Professor
2019.04 - 2024.03
Country：Japan
Professional Memberships 【 display / non-display 】
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日本香粧品学会
2024.04
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日本薬学会
2024.04
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日本薬剤学会
2024.04
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日本MRS学会
2020.04
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高分子学会
2014.09
Papers 【 display / non-display 】
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Organic-inorganic hybrid carbonate apatite capsules combining environment-responsive drug release and osteogenic induction for bone regeneration. Reviewed International journal
Takuro Aikawa, Syuuhei Komatsu, Maorui Zhang, Shuhei Abe, Taka-Aki Asoh, Keiji Itaka, Akihiko Kikuchi
Journal of controlled release : official journal of the Controlled Release Society 389 114423 - 114423 2025.11
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal)
Bone defect repair requires biomaterials that simultaneously regenerate bone tissue and deliver localized drug therapy. In this study, we developed drug-loadable organic-inorganic hybrid capsules consisting of a carbonate apatite (CO₃Ap) shell and a thermoresponsive coacervate core. The capsules have a distinct core-shell structure, with coacervate droplets present in the core and the shell formed from CO₃Ap. This structure enables selective drug loading, sequestering hydrophobic low molecules in the core while efficiently adsorbing bone morphogenetic proteins, such as bone morphogenetic protein-2 (BMP-2), to the shell. Release studies revealed microenvironment-responsive behavior, characterized by rapid release of small molecules under acidic conditions mimicking bone remodeling and sustained release of BMP-2 at physiological pH. In vitro studies using MC3T3-E1 preosteoblast cells confirmed excellent cytocompatibility. Furthermore, CO₃Ap capsules significantly promoted osteogenic differentiation by increasing the expression of alkaline phosphatase, osteopontin, and osteocalcin. In vivo implantation into rat mandibular defects further promoted bone regeneration, with significant increases in bone mass and osteocalcin expression compared with control groups filled with free BMP-2 or capsules alone. These findings establish CO₃Ap capsules as a bone-regenerating biomaterial that combines low molecule and protein delivery with osteoinductive capabilities. This dual-functional platform offers a versatile and clinically relevant strategy for bone defect repair and serves as the foundation for next-generation therapeutic biomaterials.
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Effect of Application of Ultrafine Water Droplets on Water Content in the Stratum Corneum Layer in Excised Human Skin and on Barrier Function in a Three-Dimensional Cultured Human Skin Model. Reviewed International journal
Hiroaki Todo, Moe Hirota, Madoka Kage, Yutaka Takagi, Ibuki Narita, Shinsuke Inoue, Yuki Tabata, Keiko Yokoyama, Shoko Itakura, Syuuhei Komatsu
Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI) 31 ( 10 ) e70223 2025.10
Authorship：Last author Language：English Publishing type：Research paper (scientific journal)
BACKGROUND: Skincare using facial humidifiers is gaining attention and has become more popular in Japan as a beauty treatment. A novel type of face humidifier capable of generating ultrafine water droplets has been developed recently. MATERIALS AND METHODS: The change in distribution of sprayed ultrafine water droplets in the stratum corneum of excised human skin over the application period was investigated using confocal Raman microscopy. In addition, changes in trans-epidermal water loss (TEWL) value were evaluated after treatment with ultrafine water droplets using a three-dimensional cultured human skin model (3D skin model). Furthermore, the effect of gene expression related to ceramide production in the skin and ceramide production were also evaluated using a 3D skin model by real-time PCR and high-performance thin-layer chromatography, respectively. RESULTS: A higher water content at the surface of the skin was confirmed over 45 min after the application. Furthermore, the TEWL value after the application of ultrafine water droplets was sharply decreased. Increased gene expression related to ceramide production was confirmed compared with the application of purified water, and increased levels of ceramide NS were observed. CONCLUSION: These results suggested that the application of ultrafine water droplets on the skin surface may be useful to maintain a physiologically good skin barrier condition.
DOI： 10.1111/srt.70223
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Manami Nomura, Junki Tomita, Masahiro Sugino, Syuuhei Komatsu, Hiroaki Todo, Nao Kodama, Yutaka Inoue
AAPS Open 11 ( 1 ) 2025.08
Language：English Publishing type：Research paper (scientific journal) Publisher：Springer Science and Business Media LLC
Abstract
Purpose
Topical local anesthetics have become increasingly important with the recent development of laser therapy. Lidocaine, the most commonly used anesthetic in clinical practice, not only has analgesic effects but also possesses properties such as suppression of neonatal seizures and antiarrhythmic, anti-inflammatory, antibacterial, and antifungal effects. This study aimed to improve the solubility and permeability of lidocaine (Lid) by preparing solid dispersions of Lid and ascorbic acid 2-glucoside (AG) and evaluating their physicochemical properties, solubility, and permeability.
Methods
Here, we evaluated the physicochemical properties and solubility of solid dispersions of Lid and AG prepared by freeze-drying (freeze-dried [FD] Lid/SSA = 1/1).
Results
For Differential scanning calorimetry measurements revealed the disappearance of the endothermic peaks associated with the melting of Lid and AG in the freeze-dried formulation (FD) (Lid/AG = 1/1), with the emergence of a new glass transition point. Powder X-ray diffraction measurements confirmed the absence of the characteristic peaks of both Lid and AG in the FD (Lid/AG = 1/1), showing a halo pattern. Near-infrared measurements indicated peak shifts and broadening of the -CH and -NH groups of Lid and the -OH and -CH groups of AG in the FD (Lid/AG = 1/1), suggesting the involvement of complex formation. Solubility tests revealed that the solubility of FD (Lid/AG = 1/1) was approximately 220 mg/mL, which is approximately 50 times higher than that of Lid (approximately 4.0 mg/mL). Nuclear Overhauser effect spectroscopy nuclear magnetic resonance (NMR) measurements revealed cross-peaks between the -CH groups of Lid and the -OH groups of AG, suggesting intermolecular interactions via hydrogen bonding. Diffusion-ordered spectroscopy NMR measurements showed that the diffusion coefficients of Lid and AG in the FD (Lid/AG = 1/1) converged, indicating that the formation of a complex with AG altered the dispersion behavior of Lid in the solvent. In the silicon membrane permeability test, the cumulative amount permeated after 24 h was approximately 700 µg/cm2 for Lid, compared to approximately 4.0 µg/cm2 for FD (Lid/AG = 1/1), suggesting that membrane permeability was inhibited.
Conclusion
These findings suggest that complex formation occurred in FD Lid/ AG; this enhanced the solubility, membrane permeability was inhibited of this dispersion.
Graphical AbstractDOI： 10.1186/s41120-025-00119-1
Other Link： https://link.springer.com/article/10.1186/s41120-025-00119-1/fulltext.html
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Syuuhei Komatsu, Yuya Mizuno, Akihiko Kikuchi
ACS Applied Bio Materials 8 ( 8 ) 7261 - 7269 2025.07
Authorship：Lead author, Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：American Chemical Society (ACS)
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Preparation of thermoresponsive core-corona particles for controlled phagocytosis via surface properties and particle shape transformation Invited Reviewed International journal
Syuuhei Komatsu, Takuma Suzuki, Yota Kosukegawa, Masatoshi Kawase, Takuya Matsuyama, Taka-Aki Asoh, Akihiko Kikuchi
Journal of Controlled Release 381 113652 - 113652 2025.03
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal) Publisher：Elsevier BV
Cell-particle interactions, such as phagocytosis, exhibit variability based on particle shape, surface physical properties, and diameter. These interactions can be intentionally modified through in situ change in the physical characteristics of the particulate materials. By manipulating both the surface properties and shape of the particles, it may be feasible to regulate their interactions with cells. Objective of this research is to prepare thermoresponsive core-corona particles those undergo transformation and alteration in surface solubility near physiological temperature and to investigate particle shape- and surface physical property-dependent phagocytosis. The glass transition temperature of the prepared particles was controlled via the composition of the polymer core. Rod-type particles, prepared by uniaxially stretching particle-containing films at above the glass transition temperature of the core-forming materials, demonstrated reduced phagocytosis by macrophages compared to that of spherical particles. Furthermore, the physical properties of the particle surface exerted a significant influence on phagocytosis, with hydrophobic particles being more readily engulfed. Consequently, precise control of phagocytosis can be controlled by manipulating the particle's shape and surface properties. The prepared particles have potential applications as drug delivery system carriers, enabling the regulation of cell interactions via particle shape and surface physical properties induced by temperature changes.
Books and Other Publications 【 display / non-display 】
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タンパク質、細胞の吸着制御技術
小松周平, 菊池明彦（ Role： Joint author , 第2章7節 タンパク質吸着抑制能を有する分解性ハイドロゲル表面の構築）
技術情報協会 2024.09 （ ISBN:9784867980408 ）
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刺激応答性高分子の開発動向
荏原充宏、宮田隆志、児島千恵、竹岡敬和、大﨑基史、原田 明、髙島義徳、金 昌明、朴 峻秀、菊池明彦、小松周平、大山陽介、今任景一、山門陵平、前田大光、大矢裕一、粕谷有造、池田英里子、吉原愛澄、増田 造、坂本和歌子、嶋田直彦、丸山 厚、中川泰宏、藤澤七海、下元浩晃、井原栄治、和田健彦、遊佐真一 、山田創太、金澤秀子、西浦正芳、侯 召民、麻生隆彬、三輪洋平、宇田川太郎、沓水祥一、高木賢太郎、杉野卓司、釜道紀浩、菊地邦友、宇都甲一郎、小土橋陽平、伊藤祥太郎、秋山陽久、磯田恭佑、塚原剛彦、﨑川伸基、垣内田 洋、原口和敏、野々山貴行（ Role： Joint author）
シーエムシー出版 2021.07 （ ISBN:9784781316116 ）
Misc 【 display / non-display 】
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Syuuhei Komatsu
Impact 2022 ( 1 ) 51 - 53 2022.02
Authorship：Lead author Language：English Publisher：Science Impact, Ltd.
Although there are treatments available for bone defects and the associated diseases and ailments, there are problems associated with these treatments. However, artificial bone treatments are becoming increasingly advanced thanks to scientific and technological developments. Assistant Professor Syuuhei Komatsu and Professor Akihiko Kikuchi are collaborating to design materials that can assist with improved drug treatment and bone generation. The researchers are based in Kikuchi's laboratory in the Faculty of Advanced Engineering, Tokyo University of Science, Japan, where they are exploring how biomaterials can be used to improve the outcomes of patients with bone defects. The researchers have particular expertise in organic materials but given the importance of combining inorganic and organic materials in their work, they are collaborating with doctors and other medical practitioners to develop solutions that can be quickly and effectively moved from bench to bedside. In recent work, the team is creating carbonate apatite particles with drug treatable bone reproduction ability. As part of this, the researchers are focused on inorganic hybrid particles with the expectation that these types of materials can be injected directly into the bone defect site, representing a non-invasive treatment.
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複数薬物治療可能な骨再生能を持つ炭酸アパタイト粒子の作製 Invited
Syuuhei Komatsu
40 ( 1 ) 26 - 27 2022.01
Authorship：Lead author Language：Japanese
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学会印象記 2020東海シンポジウム
小松周平
バイオマテリアル -生体材料- 39 ( 3 ) 202 2021.07
Authorship：Lead author Language：Japanese
Presentations 【 display / non-display 】
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腎細胞選択性を指向したL-セリン修飾PEG化粒子の特性解析
大塚千夏, 塩本昌平, 小松周平, 秋山好嗣, 菊池明彦
第47回日本バイオマテリアル学会大会 2025.11
Event date： 2025.11
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骨芽細胞培養のためのκーカラギーナンゲルビーズ界面修飾
後藤楓香, 塚田渉太, 塩本昌平, 小松周平, 菊池明彦
第47回日本バイオマテリアル学会大会 2025.11
Event date： 2025.11
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破骨細胞分化抑制を指向したマイクロRNA/ラクトフェリン内包高分子ミセルの調製
井伊涼子, 塩本昌平, 小松周平, 秋山好嗣, 菊池明彦
第47回日本バイオマテリアル学会大会 2025.11
Event date： 2025.11
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温度応答性微粒子とマクロファージとの相互作用解析
菊池明彦, 小松周平, 麻生隆彬
第47回日本バイオマテリアル学会大会 2025.11
Event date： 2025.11
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熱耐性獲得阻害と抗がん活性をもつ光応答性茶葉由来粒子の作製
小松周平, 鈴木啓斗, 石井花苗, 田中香帆, 鈴木龍一郎, 北村雅史, 大木和也, 高橋 淳, 藤堂浩明
第47回日本バイオマテリアル学会大会 2025.11
Event date： 2025.11
Industrial Property Rights 【 display / non-display 】
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分解性ポリマー及び医療機器
小松 周平, 菊池 明彦, 神谷 樹
Applicant：学校法人東京理科大学
Application no：特願2020-179944 Date applied：2020.10
Announcement no：特開2021-075707 Date announced：2021.05
Awards 【 display / non-display 】
Scientific Research Funds Acquisition Results 【 display / non-display 】
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骨再生のための薬物治療と細胞移植を同時に行う有機-細胞-無機複合粒子の創成
Grant number：25K15917 2025.04 - 2028.03
日本学術振興会 科学研究費助成事業 基盤研究(C)
小松 周平, 菊池 明彦
Grant amount：\4550000 （ Direct Cost: \3500000 、 Indirect Cost：\1050000 ）
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骨欠損治療を目指した骨再生能と抗菌性を持つコアシェル粒子の創製
Grant number：23K13845 2023.04 - 2025.03
日本学術振興会 科学研究費助成事業 若手研究 若手研究
小松 周平
Grant amount：\4680000 （ Direct Cost: \3600000 、 Indirect Cost：\1080000 ）
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Preparation of surface-functionalized particles and their interaction with immune cells
Grant number：21H03827 2021.04 - 2024.03
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B) Grant-in-Aid for Scientific Research (B)
Grant amount：\17810000 （ Direct Cost: \13700000 、 Indirect Cost：\4110000 ）
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骨欠損治療のための薬物治療可能な骨再生能を持つ炭酸アパタイト粒子の創製
Grant number：21K18068 2021.04 - 2023.03
日本学術振興会 科学研究費助成事業 若手研究 若手研究
小松 周平
Authorship：Principal investigator
Grant amount：\4550000 （ Direct Cost: \3500000 、 Indirect Cost：\1050000 ）
本研究では「骨欠損の治療を目指した薬物治療可能な骨再生能を持つ炭酸アパタイト粒子の作製」を目的としている。目的の達成のため、本年度では、予定通り、① 薬物の内包・放出能解析 (薬物の担持量・放出量などを蛍光分光光度計、UV-vis.などで評価)、② 骨芽細胞 (MC3T3-E1細胞)を用いて、CO3Ap粒子の骨伝導能・骨誘導能評価(アルカリフォスファターゼ活性の測定による骨誘導測定)、③マウスへのCO3Ap粒子インジェクションによる骨再生・新生骨形成評価(マウス骨内で骨形成を行い、CTスキャンによる骨再生評価)の評価を行った。
①薬物の内包・付着は、蛍光モデル低分子薬物とBMP-2を用いて、薬物内包・放出評価を行った。具体的には、rhodamine BとBMP-2の混合溶液に所定時間、炭酸アパタイト粒子をインキュベートさせ、薬物内包・放出試験を行った。中性pH下では放出はしなかったが、骨形成環境下の酸環境下では薬物放出量が増加した。
②骨伝導能・骨誘導能の評価は、BMP-2を付着させた粒子を、MC3T3-E1細胞と共培養し、骨芽細胞への分化評価をおこなった。具体的には、分化時に発現するオステオカルシンの発現量を定量し、分化評価を行った。さらに、アリザリンレッドS染色により骨結節の形成を確認した。
③マウスを用いた骨再生・新生骨形成評価を行った。具体的には、粒子をマウス背中にインジェクトし、異所性骨形成能をマイクロCTにより評価をした。BMP-2付着粒子のみ骨形成が確認された。
以上の結果より、薬物を放出可能かつ骨形成を可能とする特徴を見出し、骨欠損治療のための材料としての応用が期待できる。 -
Grant number：19K23611 2019.08 - 2021.03
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Research Activity Start-up Grant-in-Aid for Research Activity Start-up
Komatsu Syuuhei
Grant amount：\2860000 （ Direct Cost: \2200000 、 Indirect Cost：\660000 ）
In this study, we conducted an experiment for the purpose of "treatment of osteoporosis using novel carbonate apatite particles with a drug-encapsulating bone-inducing ability". The produced particles have a core-shell structure, the shell is composed of CO3Ap that dissolves and turns into bone, and the core is composed of biodegradable polymer. The shell could load a bone morphogenetic protein and the core load a hydrophobic low molecule drug. Furthermore, when osteoblasts were cultured in the presence of the prepared particles, the expression level of osteocalcin, which is a differentiation marker for bone cells, increased, and calcified nodules were confirmed. From the above, the produced particles can be expected to be applied to the treatment of osteoporosis.
Other External Funds 【 display / non-display 】
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薬物治療と骨再生の同時治療による骨欠損治療のための有機-無機ハイブリッド粒子の作製
2023.03
公益財団法人 熊谷科学技術振興財団 熊谷研究助成表彰
小松周平
Authorship：principal_investigator Grant type：Competitive
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薬物と骨再生による同時治療可能なカプセルの生体内での骨形成解析
2022.05 - 2023.03
Syuuhei Komatsu
Authorship：principal_investigator Grant type：Competitive
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Creation of precision-designed sugar-recognition particles for in-situ diagnosis of diabetes
2021.04 - 2022.03
Syuuhei Komatsu, Akihiko Kikuchi
Authorship：principal_investigator Grant type：Competitive
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自己免疫疾患治療を可能とするマクロファージ認識核酸医薬担体の創製
2020.06 - 2021.03
東京理科大学 若手共同研究助成金
小松周平
Authorship：principal_investigator Grant type：Competitive