|
Affiliation |
Faculty of Pharmaceutical Sciences Department of Pharmaceutical Technochemistry |
|
Homepage |
|
|
External Link |
|
Komatsu Shuhei
|
|
|
Research Interests 【 display / non-display 】
-
ハイドロゲル
-
Biomaterial, Polymer chemistry
-
刺激応答性高分子
-
有機-無機ハイブリッド材料
-
生分解性高分子
Research Areas 【 display / non-display 】
-
Nanotechnology/Materials / Polymer materials
-
Life Science / Biomaterials / バイオマテリアル工学
From Graduate School 【 display / non-display 】
-
Tokyo University of Science Graduate School, Division of Engineering Science Materials Engineering Doctor's Course Completed
- 2019
Country：Japan
-
Tokyo University of Science Faculty of Engineering Science Materials Engineering Doctor's Course Completed
- 2014
Country：Japan
External Career 【 display / non-display 】
-
Josai University Faculty of Pharmaceutical Sciences Department of Pharmaceutical Assistant Professor
2024.04
-
Tokyo University of Science Faculty of Advanced Engineering Department of Materials Science and Technology Assistant Professor
2019.04 - 2024.03
Country：Japan
Professional Memberships 【 display / non-display 】
-
日本薬剤学会
2024.04
-
日本香粧品学会
2024.04
-
日本MRS学会
2020.04
-
高分子学会
2014.09
-
日本DDS学会
2014.04
Papers 【 display / non-display 】
-
Thermoresponsive degradable hydrogels with renewable surfaces for protein removal Reviewed
Syuuhei Komatsu, Naoki Kamei, Akihiko Kikuchi
Biomaterials Science 13 324 - 329 2025.01
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal) Publisher：Royal Society of Chemistry (RSC)
In this paper, the synthesis of a thermoresponsive degradable gel that can effectively remove proteins by surface degradation was reported. The thermoresponsive shrinkage behavior caused decomposition near the surface, effectively removing proteins.
DOI： 10.1039/d4bm01383b
-
Plasmid DNA Delivery into the Skin via Electroporation with a Depot-Type Electrode Reviewed
Yuya Yoshida, Manami Aoki, Kalin Nagase, Koichi Marubashi, Hiroyuki Kojima, Shoko Itakura, Syuuhei Komatsu, Kenji Sugibayashi, Hiroaki Todo
Pharmaceutics 16 ( 9 ) 1219 - 1219 2024.09
Language：English Publishing type：Research paper (scientific journal) Publisher：MDPI AG
Objectives: Non-viral mediated plasmid DNA transfection by electroporation (EP) is an established method for gene transfection. In this study, the usefulness of direct EP at an intradermal (i.d.) site (DEP) with implanted electrodes to achieve a high protein expression level was investigated. In addition, DEP application with various intervals with a low application voltage was also evaluated to confirm its effect on protein expression. Methods: Green fluorescent protein (GFP)- and luciferase-encoding DNA were administrated, and GFP and luciferase were evaluated. Results: A higher protein expression level was observed after green fluorescent protein (GFP)- and luciferase-encoding DNA were delivered by i.d. injection followed by DEP application. When luciferase expression was observed with an in vivo imaging system, continuous expression was confirmed over 21 days after i.d. injection followed by DEP at 100 V. This approach provided increased gene expression levels compared with conventional EP methods via the stratum corneum layer. In addition, the effect of application voltage on luciferase expression was investigated; two-time applications (repeated DEP) at 20 V with 5 min intervals showed similar luciferase expression level to single DEP application with 100 V. Histological observations showed the skin became thicker after a single DEP at 100 V, whereas no apparent thickness changes were confirmed after repeated DEP at 20 V with 5 min intervals. Conclusions: This study revealed that direct i.d. voltage application achieved high protein expression levels even at low voltages. Skin is a promising administration site for DNA vaccines, so this approach may be effective for DNA vaccine delivery into skin tissue.
-
Formation mechanism of anisotropic gelatin hydrogel by self-assembly on oriented templates Invited Reviewed
Kohei Kawaguchi, Tamaki Maeda, Syuuhei Komatsu, Yoshihiro Nomura, Kazuki Murai
Molecular Systems Design & Engineering 9 ( 6 ) 561 - 570 2024.06
Language：English Publishing type：Research paper (scientific journal) Publisher：Royal Society of Chemistry (RSC)
The development of structurally controlled techniques inspired by the structural formation of living systems is of great importance for the fabrication of next-generation functional soft materials using environmentally friendly processes.
DOI： 10.1039/d4me00023d
-
Preparation of Degradable and Transformable Core–Corona-Type Particles that Control Cellular Uptake by Thermal Shape Change Reviewed International journal
Syuuhei Komatsu, Satoshi Yamada, Akihiko Kikuchi
ACS Biomaterials Science & Engineering 10 ( 2 ) 897 - 904 2024.01
Authorship：Lead author Publishing type：Research paper (scientific journal) Publisher：American Chemical Society (ACS)
-
The Design of Thermoresponsive Polymeric Particles Invited Reviewed
Syuuhei KOMATSU, Akihiko KIKUCHI
Journal of the Japan Society of Colour Material 96 ( 10 ) 338 - 343 2023.10
Authorship：Lead author Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Japan Society of Colour Material
Books and Other Publications 【 display / non-display 】
-
タンパク質、細胞の吸着制御技術
小松周平, 菊池明彦（ Role： Joint author , 第2章7節 タンパク質吸着抑制能を有する分解性ハイドロゲル表面の構築）
技術情報協会 2024.09 （ ISBN:9784867980408 ）
-
刺激応答性高分子の開発動向
荏原充宏、宮田隆志、児島千恵、竹岡敬和、大﨑基史、原田 明、髙島義徳、金 昌明、朴 峻秀、菊池明彦、小松周平、大山陽介、今任景一、山門陵平、前田大光、大矢裕一、粕谷有造、池田英里子、吉原愛澄、増田 造、坂本和歌子、嶋田直彦、丸山 厚、中川泰宏、藤澤七海、下元浩晃、井原栄治、和田健彦、遊佐真一 、山田創太、金澤秀子、西浦正芳、侯 召民、麻生隆彬、三輪洋平、宇田川太郎、沓水祥一、高木賢太郎、杉野卓司、釜道紀浩、菊地邦友、宇都甲一郎、小土橋陽平、伊藤祥太郎、秋山陽久、磯田恭佑、塚原剛彦、﨑川伸基、垣内田 洋、原口和敏、野々山貴行（ Role： Joint author）
シーエムシー出版 2021.07 （ ISBN:9784781316116 ）
Misc 【 display / non-display 】
-
Syuuhei Komatsu
Impact 2022 ( 1 ) 51 - 53 2022.02
Authorship：Lead author Language：English Publisher：Science Impact, Ltd.
Although there are treatments available for bone defects and the associated diseases and ailments, there are problems associated with these treatments. However, artificial bone treatments are becoming increasingly advanced thanks to scientific and technological developments. Assistant Professor Syuuhei Komatsu and Professor Akihiko Kikuchi are collaborating to design materials that can assist with improved drug treatment and bone generation. The researchers are based in Kikuchi's laboratory in the Faculty of Advanced Engineering, Tokyo University of Science, Japan, where they are exploring how biomaterials can be used to improve the outcomes of patients with bone defects. The researchers have particular expertise in organic materials but given the importance of combining inorganic and organic materials in their work, they are collaborating with doctors and other medical practitioners to develop solutions that can be quickly and effectively moved from bench to bedside. In recent work, the team is creating carbonate apatite particles with drug treatable bone reproduction ability. As part of this, the researchers are focused on inorganic hybrid particles with the expectation that these types of materials can be injected directly into the bone defect site, representing a non-invasive treatment.
-
複数薬物治療可能な骨再生能を持つ炭酸アパタイト粒子の作製 Invited
Syuuhei Komatsu
40 ( 1 ) 26 - 27 2022.01
Authorship：Lead author Language：Japanese
-
学会印象記 2020東海シンポジウム
小松周平
バイオマテリアル -生体材料- 39 ( 3 ) 202 2021.07
Authorship：Lead author Language：Japanese
Presentations 【 display / non-display 】
-
骨欠損治療のための薬物担持可能な 高分子ー無機複合材料の骨形成評価
2024.06
-
糖尿病目視診断への応用を目指す高分子微粒子 の特性評価
2024.06
-
目視診断のための DNA コロナ層を持つ顔料内包高 分子微粒子の作製
2024.06
-
腎細胞認識能を有する両親媒性ブロック共重合体 の合成と微粒子の調製
2024.06
-
マクロファージ標的部位を有する感温性高分子微 粒子と細胞との相互作用解析
2024.06
Industrial Property Rights 【 display / non-display 】
-
分解性ポリマー及び医療機器
小松 周平, 菊池 明彦, 神谷 樹
Applicant：学校法人東京理科大学
Application no：特願2020-179944 Date applied：2020.10
Announcement no：特開2021-075707 Date announced：2021.05
Awards 【 display / non-display 】
Scientific Research Funds Acquisition Results 【 display / non-display 】
-
骨欠損治療を目指した骨再生能と抗菌性を持つコアシェル粒子の創製
Grant number：23K13845 2023.04 - 2025.03
日本学術振興会 科学研究費助成事業 若手研究 若手研究
小松 周平
Grant amount：\4680000 （ Direct Cost: \3600000 、 Indirect Cost：\1080000 ）
-
Preparation of surface-functionalized particles and their interaction with immune cells
Grant number：21H03827 2021.04 - 2024.03
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B) Grant-in-Aid for Scientific Research (B)
Grant amount：\17810000 （ Direct Cost: \13700000 、 Indirect Cost：\4110000 ）
-
骨欠損治療のための薬物治療可能な骨再生能を持つ炭酸アパタイト粒子の創製
Grant number：21K18068 2021.04 - 2023.03
日本学術振興会 科学研究費助成事業 若手研究 若手研究
小松 周平
Authorship：Principal investigator
Grant amount：\4550000 （ Direct Cost: \3500000 、 Indirect Cost：\1050000 ）
本研究では「骨欠損の治療を目指した薬物治療可能な骨再生能を持つ炭酸アパタイト粒子の作製」を目的としている。目的の達成のため、本年度では、予定通り、① 薬物の内包・放出能解析 (薬物の担持量・放出量などを蛍光分光光度計、UV-vis.などで評価)、② 骨芽細胞 (MC3T3-E1細胞)を用いて、CO3Ap粒子の骨伝導能・骨誘導能評価(アルカリフォスファターゼ活性の測定による骨誘導測定)、③マウスへのCO3Ap粒子インジェクションによる骨再生・新生骨形成評価(マウス骨内で骨形成を行い、CTスキャンによる骨再生評価)の評価を行った。
①薬物の内包・付着は、蛍光モデル低分子薬物とBMP-2を用いて、薬物内包・放出評価を行った。具体的には、rhodamine BとBMP-2の混合溶液に所定時間、炭酸アパタイト粒子をインキュベートさせ、薬物内包・放出試験を行った。中性pH下では放出はしなかったが、骨形成環境下の酸環境下では薬物放出量が増加した。
②骨伝導能・骨誘導能の評価は、BMP-2を付着させた粒子を、MC3T3-E1細胞と共培養し、骨芽細胞への分化評価をおこなった。具体的には、分化時に発現するオステオカルシンの発現量を定量し、分化評価を行った。さらに、アリザリンレッドS染色により骨結節の形成を確認した。
③マウスを用いた骨再生・新生骨形成評価を行った。具体的には、粒子をマウス背中にインジェクトし、異所性骨形成能をマイクロCTにより評価をした。BMP-2付着粒子のみ骨形成が確認された。
以上の結果より、薬物を放出可能かつ骨形成を可能とする特徴を見出し、骨欠損治療のための材料としての応用が期待できる。 -
Grant number：19K23611 2019.08 - 2021.03
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Research Activity Start-up Grant-in-Aid for Research Activity Start-up
Komatsu Syuuhei
Grant amount：\2860000 （ Direct Cost: \2200000 、 Indirect Cost：\660000 ）
In this study, we conducted an experiment for the purpose of "treatment of osteoporosis using novel carbonate apatite particles with a drug-encapsulating bone-inducing ability". The produced particles have a core-shell structure, the shell is composed of CO3Ap that dissolves and turns into bone, and the core is composed of biodegradable polymer. The shell could load a bone morphogenetic protein and the core load a hydrophobic low molecule drug. Furthermore, when osteoblasts were cultured in the presence of the prepared particles, the expression level of osteocalcin, which is a differentiation marker for bone cells, increased, and calcified nodules were confirmed. From the above, the produced particles can be expected to be applied to the treatment of osteoporosis.
-
Preparation of thermoresponsive particles with shape and surface property alteration and their controlled phagocytosis
Grant number：16H03184 2016.04 - 2019.03
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
Kikuchi Akihiko, KAWASE Masatoshi, KOSUKEGAWA Yota, KOMATSU Syuuhei, ASOH Taka-Aki, ISHIHARA Ryo
Grant amount：\14040000 （ Direct Cost: \10800000 、 Indirect Cost：\3240000 ）
The objective of this research was to elucidate the effects of surface property and shape of thermoresponsive core-corona type nanoparticles on phagocytosis by macrophages. Hydrophobic particles showed larger phagocytosis than hydrophilic particles regardless of the particle shapes. However, particle shape showed greater influence on phagocytosis, spherical particles were phogocytized than rod-shaped particles regardless of surface properties of particles and hydrophobic and spherical particles showed largest phagocytosis. Such findings would be utilized to selective internalization of DDS carriers by changing shape and surface property by sole temperature changes.
Other External Funds 【 display / non-display 】
-
薬物治療と骨再生の同時治療による骨欠損治療のための有機-無機ハイブリッド粒子の作製
2023.03
公益財団法人 熊谷科学技術振興財団 熊谷研究助成表彰
小松周平
Authorship：principal_investigator Grant type：Competitive
-
薬物と骨再生による同時治療可能なカプセルの生体内での骨形成解析
2022.05 - 2023.03
Syuuhei Komatsu
Authorship：principal_investigator Grant type：Competitive
-
Creation of precision-designed sugar-recognition particles for in-situ diagnosis of diabetes
2021.04 - 2022.03
Syuuhei Komatsu, Akihiko Kikuchi
Authorship：principal_investigator Grant type：Competitive
-
自己免疫疾患治療を可能とするマクロファージ認識核酸医薬担体の創製
2020.06 - 2021.03
東京理科大学 若手共同研究助成金
小松周平
Authorship：principal_investigator Grant type：Competitive