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Faculty of Pharmaceutical Sciences Department of Pharmaceutical Technochemistry |
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Komatsu Syuuhei
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Research Interests 【 display / non-display 】
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ハイドロゲル
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Biomaterial, Polymer chemistry
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刺激応答性高分子
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有機-無機ハイブリッド材料
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生分解性高分子
Research Areas 【 display / non-display 】
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Nanotechnology/Materials / Polymer materials
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Life Science / Biomaterials / バイオマテリアル工学
From Graduate School 【 display / non-display 】
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Tokyo University of Science Graduate School, Division of Engineering Science Materials Engineering Doctor's Course Completed
- 2019
Country：Japan
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Tokyo University of Science Faculty of Engineering Science Materials Engineering Doctor's Course Completed
- 2014
Country：Japan
External Career 【 display / non-display 】
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Josai University Faculty of Pharmaceutical Sciences Department of Pharmaceutical Assistant Professor
2024.04
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Tokyo University of Science Faculty of Advanced Engineering Department of Materials Science and Technology Assistant Professor
2019.04 - 2024.03
Country：Japan
Professional Memberships 【 display / non-display 】
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日本香粧品学会
2024.04
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日本薬剤学会
2024.04
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日本薬学会
2024.04
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日本MRS学会
2020.04
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高分子学会
2014.09
Papers 【 display / non-display 】
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Preparation of thermoresponsive core-corona particles for controlled phagocytosis via surface properties and particle shape transformation Invited Reviewed International journal
Syuuhei Komatsu, Takuma Suzuki, Yota Kosukegawa, Masatoshi Kawase, Takuya Matsuyama, Taka-Aki Asoh, Akihiko Kikuchi
Journal of Controlled Release 381 113652 - 113652 2025.03
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal) Publisher：Elsevier BV
Cell-particle interactions, such as phagocytosis, exhibit variability based on particle shape, surface physical properties, and diameter. These interactions can be intentionally modified through in situ change in the physical characteristics of the particulate materials. By manipulating both the surface properties and shape of the particles, it may be feasible to regulate their interactions with cells. Objective of this research is to prepare thermoresponsive core-corona particles those undergo transformation and alteration in surface solubility near physiological temperature and to investigate particle shape- and surface physical property-dependent phagocytosis. The glass transition temperature of the prepared particles was controlled via the composition of the polymer core. Rod-type particles, prepared by uniaxially stretching particle-containing films at above the glass transition temperature of the core-forming materials, demonstrated reduced phagocytosis by macrophages compared to that of spherical particles. Furthermore, the physical properties of the particle surface exerted a significant influence on phagocytosis, with hydrophobic particles being more readily engulfed. Consequently, precise control of phagocytosis can be controlled by manipulating the particle's shape and surface properties. The prepared particles have potential applications as drug delivery system carriers, enabling the regulation of cell interactions via particle shape and surface physical properties induced by temperature changes.
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Thermoresponsive degradable hydrogels with renewable surfaces for protein removal Reviewed International journal
Syuuhei Komatsu, Naoki Kamei, Akihiko Kikuchi
Biomaterials Science 13 ( 1 ) 324 - 329 2025.01
Authorship：Lead author Language：English Publishing type：Research paper (scientific journal) Publisher：Royal Society of Chemistry (RSC)
In this paper, the synthesis of a thermoresponsive degradable gel that can effectively remove proteins by surface degradation was reported. The thermoresponsive shrinkage behavior caused decomposition near the surface, effectively removing proteins.
DOI： 10.1039/d4bm01383b
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In silico model to predict dermal absorption of chemicals in finite dose conditions. Reviewed
Ibuki Narita, Hiroaki Todo, Chihiro Fujiwara, Hiroyuki Teramae, Takeshi Oshizaka, Shoko Itakura, Syuuhei Komatsu, Kozo Takayama, Kenji Sugibayashi
The Journal of toxicological sciences 50 ( 4 ) 171 - 186 2025
Language：English Publishing type：Research paper (scientific journal)
The development of in silico approaches that can estimate the dermal absorption of chemicals exposed in practical conditions is highly anticipated. In the present study, an in silico model to estimate both the dermal absorption rate and dermal permeation profile was developed for the application of chemicals in finite dose conditions. Forty-three chemicals with molecular weights in the range 116-362 and logKo/w in the range 1.1-4.5 were used to develop an in silico model. A gradient boosting tree approach was applied to estimate permeation parameters for diffusion and partition coefficients of the chemicals in skin using physicochemical parameters of the chemicals such as molecular weight, lipophilicity, and the highest and lowest occupied molecular orbitals as the descriptor. In addition, 11 chemicals with different molecular weights and lipophilicities were applied on excised human skin in a finite dose condition, and dermal absorption profiles were obtained. Consideration of donor-solvent evaporation time, saturated concentrations of the chemicals, and donor-solvent coverage area on the skin surface, in addition to estimated skin permeation parameters of the chemicals, showed comparatively good dermal absorption profiles, although some cases of underestimation of dermal absorption were identified. It will be necessary to verify the accuracy of this model through experiments using more chemicals. However, the obtained results suggested that the established model may be valid to estimate the dermal absorption of chemicals in practical conditions.
DOI： 10.2131/jts.50.171
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Effect of the barrier function of stratum corneum and viable epidermis and dermis on the skin concentration of topically applied chemicals. Reviewed
Hiroaki Todo, Takeshi Oshizaka, Syuuhei Komatsu, Kenji Sugibayashi
The Journal of toxicological sciences 50 ( 4 ) 187 - 198 2025
Language：English Publishing type：Research paper (scientific journal)
Three-dimensional cultured skin (3D skin) models have been utilized for in vitro skin permeation tests to evaluate the skin permeation rate and local effects (efficacy and toxicity) of applied chemicals, particularly from the perspective of the 3Rs (reduction, replacement, refinement) approach. The steady-state concentration of applied chemicals at different depths in the viable epidermis and dermis (VED) is affected by their skin permeation parameters, such as permeability coefficient (Kp) and partition coefficient (K) from the donor solution to the skin of the chemicals. In the present study, the steady-state concentration of chemicals in the VED of EpiDerm 606X (EpiDerm) as representative of a 3D skin model were compared with hairless rat skin. The VED concentrations of chemicals in EpiDerm were higher than those in hairless rat skin when a model hydrophilic compound, antipyrine, and a model lipophilic compound, flurbiprofen, were applied, suggesting that the barrier functions of the VED against the whole skin were higher in EpiDerm than in hairless rat skin. When an ester compound, ethyl nicotinate, was applied, the VED concentration of nicotinic acid, a metabolite of ethyl nicotinate, was lower in EpiDerm than in hairless rat skin. These differences in the VED concentrations of applied chemicals might be related to false-positives and -negatives of topical effects evaluated with 3D skin models. It is important to pay particular attention to differences in VED concentration in 3D skin models and real skin when evaluating local efficacy and toxicity using 3D skin models.
DOI： 10.2131/jts.50.187
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Plasmid DNA Delivery into the Skin via Electroporation with a Depot-Type Electrode Reviewed International journal
Yuya Yoshida, Manami Aoki, Kalin Nagase, Koichi Marubashi, Hiroyuki Kojima, Shoko Itakura, Syuuhei Komatsu, Kenji Sugibayashi, Hiroaki Todo
Pharmaceutics 16 ( 9 ) 1219 - 1219 2024.09
Language：English Publishing type：Research paper (scientific journal) Publisher：MDPI AG
Objectives: Non-viral mediated plasmid DNA transfection by electroporation (EP) is an established method for gene transfection. In this study, the usefulness of direct EP at an intradermal (i.d.) site (DEP) with implanted electrodes to achieve a high protein expression level was investigated. In addition, DEP application with various intervals with a low application voltage was also evaluated to confirm its effect on protein expression. Methods: Green fluorescent protein (GFP)- and luciferase-encoding DNA were administrated, and GFP and luciferase were evaluated. Results: A higher protein expression level was observed after green fluorescent protein (GFP)- and luciferase-encoding DNA were delivered by i.d. injection followed by DEP application. When luciferase expression was observed with an in vivo imaging system, continuous expression was confirmed over 21 days after i.d. injection followed by DEP at 100 V. This approach provided increased gene expression levels compared with conventional EP methods via the stratum corneum layer. In addition, the effect of application voltage on luciferase expression was investigated; two-time applications (repeated DEP) at 20 V with 5 min intervals showed similar luciferase expression level to single DEP application with 100 V. Histological observations showed the skin became thicker after a single DEP at 100 V, whereas no apparent thickness changes were confirmed after repeated DEP at 20 V with 5 min intervals. Conclusions: This study revealed that direct i.d. voltage application achieved high protein expression levels even at low voltages. Skin is a promising administration site for DNA vaccines, so this approach may be effective for DNA vaccine delivery into skin tissue.
Books and Other Publications 【 display / non-display 】
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タンパク質、細胞の吸着制御技術
小松周平, 菊池明彦（ Role： Joint author , 第2章7節 タンパク質吸着抑制能を有する分解性ハイドロゲル表面の構築）
技術情報協会 2024.09 （ ISBN:9784867980408 ）
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刺激応答性高分子の開発動向
荏原充宏、宮田隆志、児島千恵、竹岡敬和、大﨑基史、原田 明、髙島義徳、金 昌明、朴 峻秀、菊池明彦、小松周平、大山陽介、今任景一、山門陵平、前田大光、大矢裕一、粕谷有造、池田英里子、吉原愛澄、増田 造、坂本和歌子、嶋田直彦、丸山 厚、中川泰宏、藤澤七海、下元浩晃、井原栄治、和田健彦、遊佐真一 、山田創太、金澤秀子、西浦正芳、侯 召民、麻生隆彬、三輪洋平、宇田川太郎、沓水祥一、高木賢太郎、杉野卓司、釜道紀浩、菊地邦友、宇都甲一郎、小土橋陽平、伊藤祥太郎、秋山陽久、磯田恭佑、塚原剛彦、﨑川伸基、垣内田 洋、原口和敏、野々山貴行（ Role： Joint author）
シーエムシー出版 2021.07 （ ISBN:9784781316116 ）
Misc 【 display / non-display 】
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Syuuhei Komatsu
Impact 2022 ( 1 ) 51 - 53 2022.02
Authorship：Lead author Language：English Publisher：Science Impact, Ltd.
Although there are treatments available for bone defects and the associated diseases and ailments, there are problems associated with these treatments. However, artificial bone treatments are becoming increasingly advanced thanks to scientific and technological developments. Assistant Professor Syuuhei Komatsu and Professor Akihiko Kikuchi are collaborating to design materials that can assist with improved drug treatment and bone generation. The researchers are based in Kikuchi's laboratory in the Faculty of Advanced Engineering, Tokyo University of Science, Japan, where they are exploring how biomaterials can be used to improve the outcomes of patients with bone defects. The researchers have particular expertise in organic materials but given the importance of combining inorganic and organic materials in their work, they are collaborating with doctors and other medical practitioners to develop solutions that can be quickly and effectively moved from bench to bedside. In recent work, the team is creating carbonate apatite particles with drug treatable bone reproduction ability. As part of this, the researchers are focused on inorganic hybrid particles with the expectation that these types of materials can be injected directly into the bone defect site, representing a non-invasive treatment.
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複数薬物治療可能な骨再生能を持つ炭酸アパタイト粒子の作製 Invited
Syuuhei Komatsu
40 ( 1 ) 26 - 27 2022.01
Authorship：Lead author Language：Japanese
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学会印象記 2020東海シンポジウム
小松周平
バイオマテリアル -生体材料- 39 ( 3 ) 202 2021.07
Authorship：Lead author Language：Japanese
Presentations 【 display / non-display 】
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がん治療のための近赤外光応答性と抗腫瘍効果を併せ持つ茶葉由来粒子の作製
鈴木 啓斗, 金井 里紗, 鈴木 龍一郎, 北村 雅史, 小松 周平, 藤堂 浩明
日本薬学会第145年会 2025.03
Event date： 2025.03
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骨再生を目指した高分子濃厚相をベースとした有機無機複合材料の作製 Invited
小松周平
日本セラミックス協会2025年年会 第9回バイオ関連デザイン研究会
Event date： 2025.03
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糖突出型DNA修飾金ナノ粒子による腫瘍マーカーの目視酵素活性評価
福森泰地, 高橋雄大, 小松周平, 宝田徹, 前田瑞夫, 菊池明彦, 秋山好嗣
核酸医薬研究センター/再生医療を加速する超細胞・DDS開発研究部門 合同第1回シンポジウム
Event date： 2024.12
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骨粗鬆症治療を目指した細胞移植用ゲルビーズの作製
塚田渉太, 小松周平, 塩本昌平, 西野達哉, 菊池明彦
核酸医薬研究センター/再生医療を加速する超細胞・DDS開発研究部門 合同第1回シンポジウム
Event date： 2024.12
Industrial Property Rights 【 display / non-display 】
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分解性ポリマー及び医療機器
小松 周平, 菊池 明彦, 神谷 樹
Applicant：学校法人東京理科大学
Application no：特願2020-179944 Date applied：2020.10
Announcement no：特開2021-075707 Date announced：2021.05
Awards 【 display / non-display 】
Scientific Research Funds Acquisition Results 【 display / non-display 】
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骨欠損治療を目指した骨再生能と抗菌性を持つコアシェル粒子の創製
Grant number：23K13845 2023.04 - 2025.03
日本学術振興会 科学研究費助成事業 若手研究 若手研究
小松 周平
Grant amount：\4680000 （ Direct Cost: \3600000 、 Indirect Cost：\1080000 ）
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Preparation of surface-functionalized particles and their interaction with immune cells
Grant number：21H03827 2021.04 - 2024.03
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B) Grant-in-Aid for Scientific Research (B)
Grant amount：\17810000 （ Direct Cost: \13700000 、 Indirect Cost：\4110000 ）
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骨欠損治療のための薬物治療可能な骨再生能を持つ炭酸アパタイト粒子の創製
Grant number：21K18068 2021.04 - 2023.03
日本学術振興会 科学研究費助成事業 若手研究 若手研究
小松 周平
Authorship：Principal investigator
Grant amount：\4550000 （ Direct Cost: \3500000 、 Indirect Cost：\1050000 ）
本研究では「骨欠損の治療を目指した薬物治療可能な骨再生能を持つ炭酸アパタイト粒子の作製」を目的としている。目的の達成のため、本年度では、予定通り、① 薬物の内包・放出能解析 (薬物の担持量・放出量などを蛍光分光光度計、UV-vis.などで評価)、② 骨芽細胞 (MC3T3-E1細胞)を用いて、CO3Ap粒子の骨伝導能・骨誘導能評価(アルカリフォスファターゼ活性の測定による骨誘導測定)、③マウスへのCO3Ap粒子インジェクションによる骨再生・新生骨形成評価(マウス骨内で骨形成を行い、CTスキャンによる骨再生評価)の評価を行った。
①薬物の内包・付着は、蛍光モデル低分子薬物とBMP-2を用いて、薬物内包・放出評価を行った。具体的には、rhodamine BとBMP-2の混合溶液に所定時間、炭酸アパタイト粒子をインキュベートさせ、薬物内包・放出試験を行った。中性pH下では放出はしなかったが、骨形成環境下の酸環境下では薬物放出量が増加した。
②骨伝導能・骨誘導能の評価は、BMP-2を付着させた粒子を、MC3T3-E1細胞と共培養し、骨芽細胞への分化評価をおこなった。具体的には、分化時に発現するオステオカルシンの発現量を定量し、分化評価を行った。さらに、アリザリンレッドS染色により骨結節の形成を確認した。
③マウスを用いた骨再生・新生骨形成評価を行った。具体的には、粒子をマウス背中にインジェクトし、異所性骨形成能をマイクロCTにより評価をした。BMP-2付着粒子のみ骨形成が確認された。
以上の結果より、薬物を放出可能かつ骨形成を可能とする特徴を見出し、骨欠損治療のための材料としての応用が期待できる。 -
Grant number：19K23611 2019.08 - 2021.03
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Research Activity Start-up Grant-in-Aid for Research Activity Start-up
Komatsu Syuuhei
Grant amount：\2860000 （ Direct Cost: \2200000 、 Indirect Cost：\660000 ）
In this study, we conducted an experiment for the purpose of "treatment of osteoporosis using novel carbonate apatite particles with a drug-encapsulating bone-inducing ability". The produced particles have a core-shell structure, the shell is composed of CO3Ap that dissolves and turns into bone, and the core is composed of biodegradable polymer. The shell could load a bone morphogenetic protein and the core load a hydrophobic low molecule drug. Furthermore, when osteoblasts were cultured in the presence of the prepared particles, the expression level of osteocalcin, which is a differentiation marker for bone cells, increased, and calcified nodules were confirmed. From the above, the produced particles can be expected to be applied to the treatment of osteoporosis.
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Preparation of thermoresponsive particles with shape and surface property alteration and their controlled phagocytosis
Grant number：16H03184 2016.04 - 2019.03
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
Kikuchi Akihiko, KAWASE Masatoshi, KOSUKEGAWA Yota, KOMATSU Syuuhei, ASOH Taka-Aki, ISHIHARA Ryo
Grant amount：\14040000 （ Direct Cost: \10800000 、 Indirect Cost：\3240000 ）
The objective of this research was to elucidate the effects of surface property and shape of thermoresponsive core-corona type nanoparticles on phagocytosis by macrophages. Hydrophobic particles showed larger phagocytosis than hydrophilic particles regardless of the particle shapes. However, particle shape showed greater influence on phagocytosis, spherical particles were phogocytized than rod-shaped particles regardless of surface properties of particles and hydrophobic and spherical particles showed largest phagocytosis. Such findings would be utilized to selective internalization of DDS carriers by changing shape and surface property by sole temperature changes.
Other External Funds 【 display / non-display 】
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薬物治療と骨再生の同時治療による骨欠損治療のための有機-無機ハイブリッド粒子の作製
2023.03
公益財団法人 熊谷科学技術振興財団 熊谷研究助成表彰
小松周平
Authorship：principal_investigator Grant type：Competitive
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薬物と骨再生による同時治療可能なカプセルの生体内での骨形成解析
2022.05 - 2023.03
Syuuhei Komatsu
Authorship：principal_investigator Grant type：Competitive
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Creation of precision-designed sugar-recognition particles for in-situ diagnosis of diabetes
2021.04 - 2022.03
Syuuhei Komatsu, Akihiko Kikuchi
Authorship：principal_investigator Grant type：Competitive
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自己免疫疾患治療を可能とするマクロファージ認識核酸医薬担体の創製
2020.06 - 2021.03
東京理科大学 若手共同研究助成金
小松周平
Authorship：principal_investigator Grant type：Competitive