論文 - 間 祐太朗
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Satoko Enjo, Yutaro Hazama, Soichiro Kimura, Yasunori Morimoto, Hideo Ueda
Journal of Advanced Pharmaceutical Technology & Research 14 ( 2 ) 94 - 98 2023年04月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Kazutoshi Watanabe, Soichiro Kimura, Yutaro Hazama, Yasunori Morimoto, Hideo Ueda
Journal of Drug Delivery and Therapeutics 13 ( 1 ) 44 - 56 2023年01月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Society of Pharmaceutical Technocrats
The purpose of our present study was to elucidate the involvement of the direct transfer to the brain after intranasal (i.n.) administration of diazepam (DZP), and to provide findings whether i.n. the administration could be used to obtain rapid onset of pharmacological action. We determined the blood and brain kinetics of DZP after administrations of i.n. and other routes, and the relationship between their concentrations and rapid seizure suppression effect; furthermore, and evaluated the distribution process of DZP to the brain. There was a negative connection between the plasma concentration and the amount of DZP delivery to the brain from the systemic circulation during the period that followed intravenous and intrarectal injection before the onset of seizure suppression. I.n. administration resulted in the seizure suppression time below the correlation curve: the seizure suppression effects were observed earlier than estimated despite the low plasma concentrations of DZP, suggesting the involvement of direct nose-to-brain delivery of DZP. The time to maximum concentration (Tmax) in the forebrain, hindbrain, olfactory bulb (OB), trigeminal nerve (TN), and cerebrospinal fluid (CSF) after i.n. the administration was 3 min, which was shorter than the Tmax of the plasma concentration. In fluorescence imaging using Rhodamin-B-base, the TN and the area of the vicinity of the thalamus had stronger fluorescence 1 and 3 min after i.n. administration, compared with the OB and CSF. In summary, direct brain delivery of DZP requires the TN and it was suggested that distribution to the vicinity of the thalamus via the TN may be connected to the quick seizure suppression effect.
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Data on the activity of place cells in the hippocampal CA1 subfield of a monkey performing a shuttling task 査読あり 国際誌
間 祐太朗, 田村 了以
Data in Brief 26 1044672 2019年10月
担当区分：筆頭著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Elsevier
This data article provides spike-timestamps of place cells recorded in a male Japanese monkey and the animal's sequential positions during the performance of a shuttling task on a rectangular track. All data were recorded in the right hippocampal CA1 subfield, while the monkey performed the task under a freely behaving condition. These were the source data on the monkey place cells in our related research article entitled "Effects of self-locomotion on the activity of place cells in the hippocampus of a freely behaving monkey" [1]. In addition, here we show a movement directional activity of a place cell in two-dimensional space as an example of data utilization. The source data are freely accessible [2] and can be used by other researchers to obtain new insights into place cells, such as functional differences between animal species.
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Effects of self-locomotion on the activity of place cells in the hippocampus of a freely behaving monkey. 査読あり 国際誌
Yutaro Hazama, Ryoi Tamura
Neuroscience Letters 701 32 - 37 2019年05月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Elsevier
The activity of hippocampal cell assemblies is considered to function as the neural substrate for a cognitive map in various animal species. The firing rate of hippocampal place cells increases when an individual animal reaches a specific location in an environment. Although cumulative views on place cells have been revealed by studies using rodents under free-behavior conditions, few studies have addressed the spatial representation provided by hippocampal neurons in primates. Moreover, although previous work in rats has demonstrated the importance of locomotion velocity and direction in the spatially selective discharge of hippocampal neurons, it remains unknown whether a corresponding phenomenon exists in the primate hippocampus. In the present study, we recorded the activity and investigated the spatial representation of the hippocampal neurons in a freely behaving monkey performing a shuttle-movement task. We observed increased activity in a subset of hippocampal neurons (place cells) when the monkey crossed a particular location. Many of the monkey place cells exhibited sensitivity to locomotion velocity rather than to locomotion direction. These results suggest the existence of primate hippocampal place cells comparable to those in rodents, with the exception that, in primates, velocity information has a stronger impact on place cell activity than directional information.
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Preclinical study of tacrolimus ointment for prevention of its systemic absorption in atopic dermatitis model mice according to their skin conditions 査読あり
Yutaro Hazama, Wataru Uchida, Toshihisa Maekawa,Ryotaro Miki, Shinji Oshima, Yuya Egawa, Osamu Hosoya, Toshinobu Seki
Japanese Journal of Pharmaceutical Health Care and Sciences 43 ( 9 ) 477 - 491 2017年09月
担当区分：筆頭著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Japanese Society of Pharmaceutical Health Care and Sciences
Tacrolimus (TL) is used topically for atopic dermatitis (AD) treatment. In AD, the skin shows various physiological alterations across individuals, body sites, and time-courses. Our previous study using rats showed that alterations in the skin barrier function and skin blood flow affected systemic absorption of topically applied TL. In this study, we performed an in vivo skin absorption study using NC/Nga mice repeatedly administered Dermatophagoides farinae extract as an AD animal model to assess skin barrier function and skin blood flow. We used three types of TL ointment: or iginal TL ointment (Protopic® 0.1％ ointment) and liquid paraffin-diluted TL ointment with or without adrenaline (0.5w /w％) to suppress systemic absorption of TL. Skin barrier function correlated positively with the systemic absorption of TL in AD skin as well as in tape-stripped rat skin, and dilution of TL ointment suppressed TL absorption and showed high skin TL retention. Although skin blood flow affected TL absorption as the skin barrier was disrupted, the distinct relationship among skin blood flow, TL absorption, and the effect of combined-use of adrenaline was unclear. The relationship between blood and skin disposition of TL was biphasic—the ratio of blood to skin disposition increased rapidly at an inflection point. These results demonstrate that the dilution of TL ointment is useful for increasing treatment safety while retaining efficacy. The observed relationship between TL behavior and skin barrier function in AD model mice may also occur in AD patients and needs to be confirmed in further studies.
その他リンク： http://libir.josai.ac.jp/il/meta_pub/G0000284repository_JOS-jjphcs.43.477
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タクロリムス軟膏の安全な使用を志向した皮膚状態が異なる部位に適用した場合の全身および皮膚局所移行動態の解析
間 祐太朗
2016年03月
記述言語：日本語 掲載種別：学位論文（博士）
その他リンク： http://libir.josai.ac.jp/il/meta_pub/G0000284repository_JOS-PhDK68
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Effect of physiological changes in the skin on systemic absorption of tacrolimus following topical application in rats 査読あり 国際誌
Yutaro Hazama, Toshihisa Maekawa, Ryotaro Miki, Shinji Oshima, Yuya Egawa, Kazuhiro Morimoto, Toshinobu Seki
Biological and Pharmaceutical Bulletin 39 ( 3 ) 343 - 352 2016年03月
担当区分：筆頭著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：The Pharmaceutical Society of Japan
Tacrolimus (TL) ointment is a topical treatment for atopic dermatitis, a disease that exhibits various skin conditions. The effect of skin pathologies on the systemic absorption of TL and related side effects remains unknown. This study aimed to investigate factors affecting the cutaneous absorption of TL. We prepared various skin models in hairless rats by tape stripping, injection of prophlogistic material solution (PMS), and continuous subcutaneous adrenaline (Adr) infusion. In vivo absorption studies were conducted, with measurements of transepidermal water loss (TEWL) and skin blood flow as physiological parameters. Very little TL absorption was observed through intact skin. Greater TL absorption was noted in skins with high TEWL values and fully stripped skin with PMS injections. In contrast, Adr infusion, which reduced skin blood flow, resulted in decreased TL absorption through fully stripped skin. Combined use of TL and Adr on skin with PMS injections resulted in suppression of TL absorption. Our results revealed that TL absorption following topical application is affected by alterations in the skin barrier, blood flow, and vascular permeability. We propose an administration plan for TL in a flowchart as a means of preventing systemic side effects.
その他リンク： http://libir.josai.ac.jp/il/meta_pub/G0000284repository_JOS-bpb.b15-00727
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フェンタニル貼付剤の粘着性に関する検討―貼付試験と粘着性による評価― 査読あり
大島 新司, 間 祐太朗, 細谷 治, 長島 健悟, 田中 享, 佐野 元彦, 太田 昌一郎, 安野 伸浩, 從二 和彦
日本緩和医療薬学雑誌 5 ( 1 ) 7 - 13 2012年03月
担当区分：第二著者 記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：日本緩和医療薬学会
フェンタニル貼付剤は、その製剤学的特性から、経口摂取困難な患者や在宅緩和ケア領域で、持続痛をコントロールするのに非常に適した製剤である。貼付期間中の剥れや痒みは、除痛効果の低下や貼付部位の不快感を誘発する。しかしながら、それら使用感に関する報告はあまり多く見受けられない。今回われわれは、3種類のフェンタニル貼付剤について、使用感(貼付時の感覚)調査および粘着性に関する研究を行った。フェントステープは、その高い粘着性のため剥離後の皮膚刺激感を与えることが示唆された。一方、デュロテップMTパッチおよびワンデュロパッチの部分的な剥離や脱落は、それらの粘着性の低さが要因と考えられた。がん対策基本法施行以来、緩和ケアにおけるがん疼痛治療期間が長期に及ぶ傾向にあり、本研究結果は、患者のアドヒアランスの向上や適切な薬物療法の維持のために重要な情報を与えるものと考える。
その他リンク： http://libir.josai.ac.jp/il/meta_pub/G0000284repository_JOS-18829783_5_7