ARAI Takeshi



Faculty of Pharmaceutical Sciences Department of Clinical Dietetics and Human Nutrition


Assistant Professor(Moving Out or Retirement)

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Degree 【 display / non-display

  • 博士(薬学) ( 2010.03   城西大学 )

  • 修士(医療栄養学) ( 2007.03   城西大学 )

  • 学士(医療栄養学) ( 2005.03   城西大学 )

Research Interests 【 display / non-display

  • 高齢者

  • 摂食嚥下

  • 低栄養

Research Areas 【 display / non-display

  • Others / Others  / 栄養学および健康科学関連

From School 【 display / non-display

  • Josai University   Faculty of Pharmaceutical Science   Graduated

    - 2005.03

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From Graduate School 【 display / non-display

  • Josai University   Graduate School, Division of Pharmaceutical Sciences   Doctor's Course   Completed

    - 2010.03

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  • Josai University   Graduate School, Division of Pharmaceutical Sciences   Master's Course   Completed

    - 2007.03

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Employment Record in Research 【 display / non-display

  • Josai University   Faculty of Pharmaceutical Sciences   Department of Clinical Dietetics and Human Nutrition   Assistant Professor


External Career 【 display / non-display

  • 医療法人晴生会 介護老人保健施設   葵の園・八千代   管理栄養士


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  • 医療法人晴生会 介護老人保健施設   葵の園・常総   管理栄養士

    2013.11 - 2018.03

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  • Jichi Medical University

    2010.04 - 2013.10

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Professional Memberships 【 display / non-display

  • 日本栄養改善学会


  • 日本摂食嚥下リハビリテーション学会


  • 日本栄養士会(茨城県栄養士会)


  • 日本動脈硬化学会

    2007.04 - 2014.03

  • 日本栄養食糧学会

    2006.04 - 2016.12

Qualification Acquired 【 display / non-display

  • Administrative Dietitian


Research Career 【 display / non-display

  • 六君子湯による食欲不振改善の作用機序の解明

    (not selected)  

    Project Year: 2010.04  -  2013.10 

  • 魚油とフェノフィブラートの糖・脂質代謝制御システムに及ぼす影響

    (not selected)  

    Project Year: 2005.04  -  2010.03 

Papers 【 display / non-display

  • GLP-1 release and vagal afferent activation mediate the beneficial metabolic and chronotherapeutic effects of D-allulose. Reviewed

    Iwasaki Yusaku, Sendo Mio, Dezaki Katsuya, Hira Tohru, Sato Takehiro, Nakata Masanori, Goswami Chayon, Aoki Ryohei, Arai Takeshi, Kumari Parmila, Hayakawa Masaki, Masuda Chiaki, Okada Takashi, Hara Hiroshi, Drucker Daniel J, Yamada Yuichiro, Tokuda Masaaki, Yada Toshihiko

    Nat Commun   9 ( 1 )   113 - 113   2018.01

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    Language:English   Publishing type:Research paper (scientific journal)  

    Overeating and arrhythmic feeding promote obesity and diabetes. Glucagon-like peptide-1 receptor (GLP-1R) agonists are effective anti-obesity drugs but their use is limited by side effects. Here we show that oral administration of the non-calorie sweetener, rare sugar D-allulose (D-psicose), induces GLP-1 release, activates vagal afferent signaling, reduces food intake and promotes glucose tolerance in healthy and obese-diabetic animal models. Subchronic D-allulose administered at the light period (LP) onset ameliorates LP-specific hyperphagia, visceral obesity, and glucose intolerance. These effects are blunted by vagotomy or pharmacological GLP-1R blockade, and by genetic inactivation of GLP-1R signaling in whole body or selectively in vagal afferents. Our results identify D-allulose as prominent GLP-1 releaser that acts via vagal afferents to restrict feeding and hyperglycemia. Furthermore, when administered in a time-specific manner, chronic D-allulose corrects arrhythmic overeating, obesity and diabetes, suggesting that chronotherapeutic modulation of vagal afferent GLP-1R signaling may aid in treating metabolic disorders.

    DOI: 10.1038/s41467-017-02488-y,


  • Fish oil and fenofibrate inhibit pancreatic islet hypertrophy, and improve glucose and lipid metabolic dysfuntions with different ways in diabetic KK mice. Reviewed

    Nakasatomi Maki, Kim Hyounju, Arai Takeshi, Hirako Satoshi, Shioda Seiji, Iizuka Yuzuru, Sakurai Koji, Matsumoto Akiyo

    Obes Res Clin Pract   12 ( 1S1 )   29 - 38   2018.01

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    Language:English   Publishing type:Research paper (scientific journal)  

    We examined the effects of fish oil and fenofibrate (FF) on the pancreatic islet hypertrophy, and on the modification of glucose and lipid metabolic dysfunctions in KK mice with insulin resistance. The mice were fed one of four diets [25en% lard/safflower oil (LSO), 25en% fish oil (FO), or each of these diets plus 0.1wt% FF (LSO/FF, FO/FF)] for 9 weeks. FO group and both FF groups had significantly lower final body and adipose tissue weights than LSO group. Pancreatic islet hypertrophy was observed only in LSO group but not in the other groups with fish oil or FF. And, it is likely that fish oil has a stronger therapeutic effect on islet hypertrophy. Plasma adiponectin level was significantly higher in FO group but not in both FF groups. Expression of hepatic lipogenic enzyme genes such as fatty acid synthase (FAS) and stearoyl-CoA desaturase-1 (SCD-1) was lower in FO groups with or without FF, whereas fatty acid oxidation-related mRNAs such as acyl-CoA oxidase (AOX) and uncoupling protein-2 (UCP-2) were more abundant in FF groups with or without fish oil. Our results suggest that both fish oil and FF improve pancreatic islet hypertrophy with the amelioration of insulin resistance. Fish oil enhances insulin sensitivity by increasing plasma adiponectin; however, the beneficial effect of FF on insulin resistance seems to be independent of the plasma adiponectin level. These results mean that improvement of glucose and lipid metabolic dysfuctions in diabetic KK mice are independently approached by fish oil and FF.

    DOI: 10.1016/j.orcp.2016.03.012


  • Arcuate Na+,K+-ATPase senses systemic energy states and regulates feeding behavior through glucose-inhibited neurons. Reviewed

    Kurita Hideharu, Xu Kai Y, Maejima Yuko, Nakata Masanori, Dezaki Katsuya, Santoso Putra, Yang Yifei, Arai Takeshi, Gantulga Darambazar, Muroya Shinji, Lefor Alan K, Kakei Masafumi, Watanabe Eiju, Yada Toshihiko

    Am J Physiol Endocrinol Metab   309 ( 4 )   E320 - E333   2015.08

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    Language:English   Publishing type:Research paper (scientific journal)  

    Feeding is regulated by perception in the hypothalamus, particularly the first-order arcuate nucleus (ARC) neurons, of the body's energy state. However, the cellular device for converting energy states to the activity of critical neurons in ARC is less defined. We here show that Na(+),K(+)-ATPase (NKA) in ARC senses energy states to regulate feeding. Fasting-induced systemic ghrelin rise and glucose lowering reduced ATP-hydrolyzing activity of NKA and its substrate ATP level, respectively, preferentially in ARC. Lowering glucose concentration (LG), which mimics fasting, decreased intracellular NAD(P)H and increased Na(+) concentration in single ARC neurons that subsequently exhibited [Ca(2+)]i responses to LG, showing that they were glucose-inhibited (GI) neurons. Third ventricular injection of the NKA inhibitor ouabain induced c-Fos expression in agouti-related protein (AgRP) neurons in ARC and evoked neuropeptide Y (NPY)-dependent feeding. When injected focally into ARC, ouabain stimulated feeding and mRNA expressions for NPY and AgRP. Ouabain increased [Ca(2+)]i in single NPY/AgRP neurons with greater amplitude than in proopiomelanocortin neurons in ARC. Conversely, the specific NKA activator SSA412 suppressed fasting-induced feeding and LG-induced [Ca(2+)]i increases in ARC GI neurons. NPY/AgRP neurons highly expressed NKAalpha3, whose knockdown impaired feeding behavior. These results demonstrate that fasting, via ghrelin rise and LG, suppresses NKA enzyme/pump activity in ARC and thereby promotes the activation of GI neurons and NPY/AgRP-dependent feeding. This study identifies ARC NKA as a hypothalamic sensor and converter of metabolic states to key neuronal activity and feeding behaviour, providing a new target to treat hyperphagic obesity and diabetes.

    DOI: 10.1152/ajpendo.00446.2014


  • Peripheral oxytocin activates vagal afferent neurons to suppress feeding in normal and leptin-resistant mice: a route for ameliorating hyperphagia and obesity. Reviewed

    Iwasaki Yusaku, Maejima Yuko, Suyama Shigetomo, Yoshida Masashi, Arai Takeshi, Katsurada Kenichi, Kumari Parmila, Nakabayashi Hajime, Kakei Masafumi, Yada Toshihiko

    Am J Physiol Regul Integr Comp Physiol   308 ( 5 )   R360 - R369   2015.03

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    Language:English   Publishing type:Research paper (scientific journal)  

    Oxytocin (Oxt), a neuropeptide produced in the hypothalamus, is implicated in regulation of feeding. Recent studies have shown that peripheral administration of Oxt suppresses feeding and, when infused subchronically, ameliorates hyperphagic obesity. However, the route through which peripheral Oxt informs the brain is obscure. This study aimed to explore whether vagal afferents mediate the sensing and anorexigenic effect of peripherally injected Oxt in mice. Intraperitoneal Oxt injection suppressed food intake and increased c-Fos expression in nucleus tractus solitarius to which vagal afferents project. The Oxt-induced feeding suppression and c-Fos expression in nucleus tractus solitarius were blunted in mice whose vagal afferent nerves were blocked by subdiaphragmatic vagotomy or capsaicin treatment. Oxt induced membrane depolarization and increases in cytosolic Ca(2+) concentration ([Ca(2+)]i) in single vagal afferent neurons. The Oxt-induced [Ca(2+)]i increases were markedly suppressed by Oxt receptor antagonist. These Oxt-responsive neurons also responded to cholecystokinin-8 and contained cocaine- and amphetamine-regulated transcript. In obese diabetic db/db mice, leptin failed to increase, but Oxt increased [Ca(2+)]i in vagal afferent neurons, and single or subchronic infusion of Oxt decreased food intake and body weight gain. These results demonstrate that peripheral Oxt injection suppresses food intake by activating vagal afferent neurons and thereby ameliorates obesity in leptin-resistant db/db mice. The peripheral Oxt-regulated vagal afferent neuron provides a novel target for treating hyperphagia and obesity.

    DOI: 10.1152/ajpregu.00344.2014


  • Paraventricular nucleus nesfatin-1 neurons are regulated by pituitary adenylate cyclase-activating polypeptide (PACAP). Reviewed

    Maejima Yuko, Shimomura Kenju, Sakuma Kazuya, Yang Yifei, Arai Takeshi, Mori Masatomo, Yada Toshihiko

    Neurosci Lett   551   39 - 42   2013.09

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    Language:English   Publishing type:Research paper (scientific journal)  

    Nesfatin-1 is a neuropeptide localized in hypothalamic paraventricular nucleus (PVN). Previously, we have reported the mechanism of feeding suppression by nesfatin-1, and also reported the ability of nesfatin-1 in regulating stress response and the circadian feeding pattern. Pituitary adenylate cyclase-activating polypeptide (PACAP) is a neuropeptide also related to the stress response, feeding, and regulation of cardiovascular and autonomic nervous systems. The neurons with receptors for PACAP are distributed in PVN. However, there are no reports showing the direct effect of PACAP on nesfatin-1 neurons. In order to explore the direct effect of PACAP on PVN nesfatin-1 neuron, we have measured the cytosolic free calcium ([Ca(2+)]i) using fura-2 microfluorometry in single neurons isolated from PVN of adult rats, followed by immunocytochemical identification of nesfatin-1 neurons. PACAP at 10(-15)M to 10(-9)M increased [Ca(2+)]i in dose dependent manner. PAC1 and VPAC2 receptor agonists also increased [Ca(2+)]i. Sixteen out of 40 neurons (40%) in PVN responded to 10(-9)M PACAP, and 12 out of 16 neurons (75%) which responded to 10(-9)M PACAP were found to be nesfatin-1 neurons. In this paper we show that PACAP directly activates nesfatin-1 neurons in PVN. The data suggest that nesfatin-1 controls feeding, stress response or autonomic response under PACAP regulation.

    DOI: 10.1016/j.neulet.2013.07.006


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Misc 【 display / non-display

  • 視床下部弓状核・室傍核と摂食・精神. Invited

    高野 英介, 荒井 健, 矢田 俊彦

    分子精神医学   13 ( 1 )   40 - 44   2013.01

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)