UEMURA Takeshi

写真a

Affiliation

Faculty of Pharmaceutical Sciences Department of Pharmaceutical Sciences

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Degree 【 display / non-display

  • 博士(医薬学) ( 2005.03   千葉大学 )

  • 学士(薬学) ( 2000.03   東邦大学 )

  • 修士(臨床薬学) ( 2000.03   千葉大学 )

Research Interests 【 display / non-display

  • senescence

  • polyamine

Research Areas 【 display / non-display

  • Life Science / Pharmaceutical hygiene and biochemistry

From School 【 display / non-display

  • Toho University   Faculty of Pharmaceutical Science   Graduated

    1996.04 - 2000.03

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    Country:Japan

From Graduate School 【 display / non-display

  • Chiba University   Graduate School, Division of Pharmaceutical Sciences   Doctor's Course   Completed

    2002.04 - 2005.03

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    Country:Japan

  • Chiba University   Graduate School, Division of Pharmaceutical Sciences   Master's Course   Completed

    2000.04 - 2002.03

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    Country:Japan

Studying abroad experiences 【 display / non-display

  • 2007.10 - 2012.03   University of Arizona, Arizona Cancer Center   Postdoctoral Research Fellow

External Career 【 display / non-display

  • Kyoto Prefectural University of Medicine   Part-time researcher for university or other academic organization

    2014.10

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    Country:Japan

  • Chiba University   Part-time researcher for university or other academic organization

    2014.10 - 2021.03

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    Country:Japan

  • 株式会社アミンファーマ研究所   主任研究員

    2014.10 - 2021.03

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    Country:Japan

  • Kyoto Prefectural University of Medicine   Assistant Professor

    2012.04 - 2014.09

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    Country:Japan

  • University of Arizona   Arizona Cancer Center   Researcher

    2007.10 - 2012.03

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    Country:United States

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Professional Memberships 【 display / non-display

  • 日本分子生物学会

    2019.04

  • American Society for Biochemistry and Molecular Biology

    2019.02

  • 日本ポリアミン学会

    2010.06

  • 日本生化学会

    2004.04

  • 日本薬学会

    2002.04

Qualification Acquired 【 display / non-display

  • TOEIC(730)

  • Pharmacist

 

Papers 【 display / non-display

  • Correlation of polyamines, acrolein-conjugated lysine and polyamine metabolic enzyme levels with age in human liver. Reviewed

    Uemura Takeshi, Akasaka Yoshihisa, Ikegaya Hiroshi

    Heliyon   6 ( 9 )   e05031 - e05031   2020.09

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    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    The polyamines spermidine, spermine and putrescine are essential for normal cellular functions. The contents of polyamines in tissue decreased in aged mice compared to young mice. In this study, the polyamine contents and their metabolic byproduct acrolein-conjugated lysine (N (ε)-(3-formyl-3,4-dehydropiperidino)-lysine, FDP-Lys) in human liver tissue were measured and analyzed the correlation with age of the subjects. The putrescine and FDP-Lys levels were significantly increased with age. On the other hand, spermine level was decreased with age. Spermidine did not significantly correlate with age. The relative amount of spermine oxidase (SMOX) significantly correlated with the age of subjects whereas ornithine decarboxylase (ODC) and adenosylmethionine decarboxylase (AMD1) significantly reduced by the age. Our results suggested that an increase in oxidation and reduction in polyamine synthesis may cause the change of polyamine profile in the elderly.

    DOI: 10.1016/j.heliyon.2020.e05031

    PubMed

  • A search for acrolein scavengers among food components Reviewed

    Uemura T, Uchida M, Nakamura M, Shimekake M, Sakamoto A, Terui Y, Higashi K, Ishii I, Kashiwagi K, Igarashi K.

    Amino Acids   2023.02

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  • Nα-(5-Fluoro-2,4-dinitrophenyl)-L-leucinamide-derivatized LC/MS/MS Analysis of Amino Acid Enantiomers in HepG2 Cells Reviewed

    Takano Y, Takahashi M, Kobayashi M, Uemura T, Furuchi T.

    Chromatography   42 ( 3 )   143 - 149   2021

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    Language:English   Publishing type:Research paper (scientific journal)  

  • Structural change and degradation of cytoskeleton due to the acrolein conjugation with vimentin and actin during brain infarction. Reviewed

    Uemura Takeshi, Suzuki Takehiro, Ko Kenta, Nakamura Mizuho, Dohmae Naoshi, Sakamoto Akihiko, Terui Yusuke, Toida Toshihiko, Kashiwagi Keiko, Igarashi Kazuei

    Cytoskeleton (Hoboken)   77 ( 10 )   414 - 421   2020.10

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    We have found recently that dendritic spine extension is inhibited through acrolein conjugation with α- and β-tubulin proteins during brain infarction. In this current study, we looked for other acrolein-conjugated proteins in the 100,000g precipitate fraction, to clarify how cytoskeleton structure is modified by acrolein. Acrolein-conjugated proteins were sought from acrolein-treated mouse FM3A and Neuro2a cells and from tissues isolated from mouse brain infarction. It was found that vimentin was conjugated with acrolein, and the conjugated amino acid residue was Cys328, which is the only Cys residue in vimentin. It was also found that Cys207, 257, 285, and Lys118 in actin, another cytoskeleton protein, were conjugated with acrolein. The structure and localization of vimentin and actin filaments were changed greatly in infarct brain in photochemically induced thrombosis model mice and in acrolein-treated Neuro2a cells. In addition, degradation of cytoskeleton proteins was accelerated in the order vimentin > tubulin > actin in mouse brain infarction. These findings indicate that a dysfunction of the cytoskeleton by acrolein is strongly involved in the tissue damage during brain infarction, together with the apoptosis caused by glyceraldehyde-3-phosphate dehydrogenase and protein degradation by matrix metalloproteinase-9.

    DOI: 10.1002/cm.21638

    PubMed

  • Urinary Amino Acid-Conjugated Acrolein and Taurine as New Biomarkers for Detection of Dementia Reviewed

    Yoshida M, Uemura T, Mizoi M, Waragai M, Sakamoto A, Terui Y, Kashiwagi K, Igarashi K.

    J Alzheimers Dis.   2023.01

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    Authorship:Second author   Language:English   Publishing type:Research paper (scientific journal)  

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Presentations 【 display / non-display

  • Aging associated change in polyamine metabolism Invited International conference

    Takeshi Uemura, Yuka Yokota, Miki Matsunaga, Yoshihisa Akasaka, Koichi Takao, Hiroshi Ikegaya, Takemitsu Furuchi

    Sixth International Conference on Polyamines: Biochemical, Physiological and Clinical Perspectives  2022.09  Enzo Agostinelli

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    Event date: 2022.09

    Language:English   Presentation type:Oral presentation (general)  

    Venue:Tivoli, Rome   Country:Italy  

Awards 【 display / non-display

  • 博慈会 ダ・ビンチ賞

    2018.11   博慈会  

    植村武史

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    Award type:Honored in official journal of a scientific society, scientific journal  Country:Japan

Scientific Research Funds Acquisition Results 【 display / non-display

  • タンパク質翻訳合成およびアミン類代謝経路の補正による抗老化機構の解明

    Grant number:22H03515  2022.04 - 2024.03

    基盤研究(B)

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    Authorship:Coinvestigator(s)  Grant type:Competitive

  • ポリアミンが誘導するリピート病の発症機構とその制御による病態抑制効果の解明

    Grant number:22K07357  2022.04 - 2024.03

    基盤研究(C)

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    Authorship:Coinvestigator(s)  Grant type:Competitive

  • 加齢によるスペルミン分解活性化機構解明と新規抗老化物質の創出

    Grant number:22K11860  2022.04 - 2024.03

    基盤研究(C)

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    Authorship:Principal investigator  Grant type:Competitive

 

Teaching Experience 【 display / non-display

  • 中毒学

  • 医学研究方法概論

    Institution:Kyoto Prefectural University of Medicine

  • 分析化学III

    Institution:Chiba University

  • 法医学

    Institution:Kyoto Prefectural University of Medicine

  • 法医学実習

    Institution:Kyoto Prefectural University of Medicine

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Committee Memberships 【 display / non-display

  • 日本ポリアミン学会   企画運営委員会委員、学会誌編集委員  

    2014.10   

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    Committee type:Academic society