関 智宏 (セキ トモヒロ)

Seki Tomohiro

写真a

職名

助教

生年

1987年

研究室住所

〒350-0295 埼玉県坂戸市けやき台1-1、城西大学 21号館 423室

研究分野・キーワード

ドラッグデリバリーシステム、製剤学、超分子化学

メールアドレス

メールアドレス

研究室電話

049-271-8107

研究室FAX

049-271-8107

出身大学 【 表示 / 非表示

  • 2006年04月
    -
    2012年03月

    城西大学   薬学部   薬学科   卒業

出身大学院 【 表示 / 非表示

  • 2012年04月
    -
    2016年03月

    城西大学  薬学研究科  薬学専攻  博士課程  修了

取得学位 【 表示 / 非表示

  • 城西大学 -  博士(薬学)

学内職務経歴 【 表示 / 非表示

  • 2019年04月
    -
    継続中

    城西大学   薬学部   薬学科   助教  

学外略歴 【 表示 / 非表示

  • 2016年07月
    -
    2018年01月

      米国立衛生研究所   研究員

  • 2018年02月
    -
    2019年03月

      米国立衛生研究所   日本学術振興会 海外特別研究員(NIH)

所属学会・委員会 【 表示 / 非表示

  • 2012年03月
    -
    継続中
     

    日本薬剤学会

  • 2011年03月
    -
    継続中
     

    日本薬学会

  • 2011年03月
    -
    2016年07月
     

    日本DDS学会

  • 2011年03月
    -
    2016年07月
     

    日本シクロデキストリン学会

専門分野(科研費分類) 【 表示 / 非表示

  • 物理系薬学

取得資格 【 表示 / 非表示

  • 薬剤師

 

論文 【 表示 / 非表示

  • Dynamic Imaging of Glucose and Lactate Metabolism by 13C-MRS without Hyperpolarization

    Brender JR, Kishimoto S, Merkle H, Reed G, Hurd RE, Chen AP, Ardenkjaer-Larsen JH, Munasinghe J, Saito K, Seki T, Oshima N, Yamamoto K, Choyke PL, Mitchell J, Krishna MC

    Scientific Reorts   9   3410   2019年  [査読有り]

    共著

    Metabolic reprogramming is one of the defining features of cancer and abnormal metabolism is associated with many other pathologies. Molecular imaging techniques capable of detecting such changes have become essential for cancer diagnosis, treatment planning, and surveillance. In particular, 18F-FDG (fluorodeoxyglucose) PET has emerged as an essential imaging modality for cancer because of its unique ability to detect a disturbed molecular pathway through measurements of glucose uptake. However, FDG-PET has limitations that restrict its usefulness in certain situations and the information gained is limited to glucose uptake only.13C magnetic resonance spectroscopy theoretically has certain advantages over FDG-PET, but its inherent low sensitivity has restricted its use mostly to single voxel measurements unless dissolution dynamic nuclear polarization (dDNP) is used to increase the signal, which brings additional complications for clinical use. We show here a new method of imaging glucose metabolism in vivo by MRI chemical shift imaging (CSI) experiments that relies on a simple, but robust and efficient, post-processing procedure by the higher dimensional analog of singular value decomposition, tensor decomposition. Using this procedure, we achieve an order of magnitude increase in signal to noise in both dDNP and non-hyperpolarized non-localized experiments without sacrificing accuracy. In CSI experiments an approximately 30-fold increase was observed, enough that the glucose to lactate conversion indicative of the Warburg effect can be imaged without hyper-polarization with a time resolution of 12s and an overall spatial resolution that compares favorably to 18F-FDG PET.

    DOI

  • Sugar-responsive smart materials based on phenylboronic acid and cyclodextrin

    Yuya Egawa, Tomohiro Seki, Ryotaro Miki, Toshinobu Seki

    Journal of Inclusion Phenomena and Macrocyclic Chemistry   94 ( 1-2 ) 1 - 10   2019年  [招待有り]

    共著

    This review focuses on sugar-responsive materials based on phenylboronic acid (PBA) as a sugar-sensing motif and cyclodextrins (CyDs) as a basic skeleton of smart materials. PBA modified α-CyD (PBA-α-CyD) forms a supramolecular polymer through intermolecular interactions between PBA part and CyD cavity. Similarly, PBA-β-CyD forms a head-to-head dimer. Meanwhile, combining PBA-γ-CyD and polyethylene glycol (PEG) produces a molecular necklace. Additionally, combining PBA-modified PEG and native α-CyD or γ-CyD results in another type of molecular necklace. These supramolecular structures are obtained as powders, and their solubility increases in the presence of sugar. Besides the powder type, a unique gel is formed through crosslinking polyvinyl alcohol with PBA-triazole-γ-CyD (PBA-Tri-γ-CyD). This gel can contain model drug, and it shows sugar-responsive drug release. The sugar response of all of these smart materials can be explained by the concept of equilibrium. The smart materials are constructed with CyD-guest interactions. The CyD-guest equilibrium moves by a reaction between sugar and PBA moiety attached to the smart material. In these smart materials, sugar induces a dissociation in the CyD-guest interaction, and this dissociation results in sugar-induced disintegration of CyD-guest supramolecular structures.

  • Sugar-Responsive Layer-by-Layer Film Composed of Phenylboronic Acid-Appended Insulin and Poly(vinyl alcohol)

    Chemical and Pharmaceutical Bulletin   66 ( 4 ) 368 - 374   2018年  [査読有り]

    単著

    Previous studies have shown that reversible chemical bond formation between phenylboronic acid (PBA) and 1,3-diol can be utilized as the driving force for the preparation of layer-by-layer (LbL) films. The LbL films composed of a PBA-appended polymer and poly(vinyl alcohol) (PVA) disintegrated in the presence of sugar. This type of LbL films has been recognized as a promising approach for sugar-responsive drug release systems, but an issue preventing the practical application of LbL films is combining them with insulin. In this report, we have proposed a solution for this issue by using PBA-appended insulin as a component of the LbL film. We prepared two kinds of PBA-appended insulin derivatives and confirmed that they retained their hypoglycemic activity. The LbL films composed of PBA-appended insulin and PVA were successfully prepared through reversible chemical bond formation between the boronic acid moiety and the 1,3-diol of PVA. The LbL film disintegrated upon treatment with sugars. Based on the results presented herein, we discuss the suitability of the PBA moiety with respect to hypoglycemic activity, binding ability, and selectivity for D-glucose.

  • A polyrotaxane gel using boronic acid-appended γ-cyclodextrin as a hybrid cross-linker

    Wataru Uchida, Maiki Yoshikawa, Tomohiro Seki, Ryotaro Miki, Toshinobu Seki, Takashi Fujihara, Yoshihiro Ishimaru, Yuya Egawa

    Journal of Inclusion Phenomena and Macrocyclic Chemistry   89 ( 3-4 ) 281 - 288   2017年  [査読有り]

    共著

    A boronic acid-appended γ-cyclodextrin (BA-CyD) was synthesized as a hybrid cross-linker of polyvinyl alcohol (PVA) to form a new type of hydrogel. The CyD moiety of BA-CyD forms an inclusion complex with the PVA chain to produce a mechanically interlocking structure. At the same time, the BA moiety of BA-CyD forms covalent bonds with the 1,3-diol moieties of PVA. On the basis of these two modes of interaction, the hybrid cross-linker connects two PVA chains, thus resulting in the formation of a hydrogel. To investigate the possibility of this hydrogel becoming the basis for an intelligent material for drug delivery, sugar-responsive drug release from the hydrogel was demonstrated.

  • Polyol-responsive pseudopolyrotaxanes based on phenylboronic acid-modified polyethylene glycol and cyclodextrins

    Yu Kojima, Tomoyuki Okano, Tomohiro Seki, Misato Namiki, Yuya Egawa, Ryotaro Miki, Kazuhiko Juni, Toshinobu Seki

    Journal of Inclusion Phenomena and Macrocyclic Chemistry   87 ( 3-4 ) 295 - 303   2017年  [査読有り]

    共著

    Phenylboronic acid (PBA), which reacts with polyols to form cyclic esters, was attached to the amino terminal of polyethylene glycol (PEG) via amide bonds. PBA-PEG was used to prepare pseudopolyrotaxanes (pPRXs) by combining it with cyclodextrins (CyDs). In the case of α-CyD, a single stranded pPRX formed that disintegrated in the presence of catechol (CA), d-fructose (Fru), and d-glucose (Glc). The order of response was CA > Fru > Glc, which corresponds with the affinities between the PBA moiety and the polyols. In contrast, a pPRX using γ-CyD, which has a double-stranded structure, showed sugar-induced disintegration but did not show a response to CA. We explained these apparently curious responses of the pPRXs using a mechanism based on the penetrability of the polyol-bound PBA toward the cavities of the CyDs. The pPRXs, which are a class of molecular machine, show two selectivities; one is derived from polyol selectivity, and the other is based on the penetrability for CyDs.

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学術関係受賞 【 表示 / 非表示

  • JSPS Fellowship for Japanese Biomedical and Behavioral Researchers Fellowship at NIH Award

    2018年08月   Japan Society for the Promotion of Science (JSPS)  

    受賞者:  Tomohiro Seki

  • Excellent Poster Presentation Award GOLD Prize

    2014年11月   第20回創剤フォーラム若手研究会 (日本薬剤学会)  

    受賞者:  関 智宏

  • ポスター賞

    2014年09月   第31回シクロデキストリンシンポジウム (日本シクロデキストリン学会)  

    受賞者:  関 智宏

  • 若手研究奨励賞

    2014年07月   物理系薬学部会 (フィジカルファーマフォーラム2014)  

    受賞者:  関 智宏

  • Most Impressive Debater賞

    2014年05月   日本薬剤学会第29年会  

    受賞者:  関 智宏

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